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Chakrabarti, Santanu
- Functional Annotation of Pathogenesis Proteins in Shigella flexneri using Comparative Genomics
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Authors
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1 Department of Zoology, Government General Degree College, Singur, West Bengal, IN
2 Department of Biotechnology, St. Xavier’s College, Kolkata- 700016, IN
1 Department of Zoology, Government General Degree College, Singur, West Bengal, IN
2 Department of Biotechnology, St. Xavier’s College, Kolkata- 700016, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 1 (2020), Pagination: 65-78Abstract
The Gram-negative bacteria, Shigella species, is a predominant diarrheal pathogen and itself accounts for 15% of the diarrheal episodes occurring globally. Shigella enters the human body through ingestion of contaminated food and water and on reaching the intestine, dismantles the epithelial barrier, generating symptoms varying from mild to severe bloody diarrhea. Widespread diversity of Shigella species and the emergence of multi-drug resistant strains in recent years has made it extremely enigmatic to design a successful drug to combat shigellosis. This work focusses on comparative genomics methods to identify and annotate hypothetical proteins from the Shigella flexneri genome in quest of identifying novel druggable targets.Keywords
Shigellosis, Hypothetical Proteins, Comparative Genomics, Drug Targets.
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- Insights Into The Structure And Dynamics Of Shigella Invasion Proteins For Use As Potential Drug Targets
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PDF Views:0
Authors
Affiliations
1 Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata-700016, IN
2 Department of Zoology, Government General Degree College, Singur, West Bengal, IN
1 Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata-700016, IN
2 Department of Zoology, Government General Degree College, Singur, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 19, No 1 (2022), Pagination: 37-42Abstract
The Gram-negative bacteria, Shigella spp. has always been a pathogen of concern in the context of global burden of diarrheal diseases, with recent surveys taking the annual incidence of shigellosis to roughly 125 million cases worldwide. The scenario has been further worsened by the frequent reports on isolation of multi drug resistant (MDR) Shigella strains, showing resistance against potent antibiotics including the 3rd generation cephalosporins as well. With the recent years witnessing a growing interest in the discovery of novel drugs, this work has scrutinized the structural nitty-gritty of the predominant virulence factors in Shigella, viz. the invasion plasmid antigens IpaA, IpaB, IpaC and IpaD, that are evidenced to have multifaceted role in the pathogenesis cascade, to explore their potential as promising therapeutic targets. In this work we report the 3D models of the different invasion proteins using comparative modeling and a comprehensive structural evaluation protocol for evaluating their stability as drug targets. Our data suggest significant potential of these invasion proteins to serve as future drug targets, thus opening up the avenue for further investigations.Keywords
Shigella, Invasion proteins, Structural analysis, Drug targets, In-silico.References
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