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Conservation of Medicinally Important Plant Through Somatic Embryogenesis :Case Study with Eupatorium Ayapana Vent. (Asteraceae)


Affiliations
1 Department of Botany, Government General Degree College, Keshiary, Paschim Medinipur, PIN–721135, India
     

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An efficient plant regeneration protocol through somatic embryogenesis of leaf explants of a valuable medicinal plant, Eupatorium ayapana Vent., has been developed. Callus development from leaf discs were achieved within 4 weeks of callus initiation on MS medium fortified with different concentrations and combinations of BAP and sterile coconut water. Extensive growth of green nodular calli was recorded in MS medium supplemented with BAP (2 mg/l) and coconut water (20 ml/l). Transfer of differentiating calli into the medium containing lower concentrations of cytokinin and coconut water resulted into the development of cotyledonary stage somatic embryoids. Maturation of somatic embryoids and subsequent plant regeneration was accomplished in growth regulator free MS medium. Further, genetic fidelity of the micropropagated plants with that of the mother plant was assessed by isoenzyme profiles and their true-to-type nature was confirmed on the basis of monomorphic banding pattern.

Keywords

Medicinal Plant, Eupatorium Ayapana, Somatic Embryogenesis.
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  • [Editors' comment: Eupatorium ayapana Vent. is a synonym of Ayapana triplinervis (Vahl) R. M. King and H. Rob.].

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  • Conservation of Medicinally Important Plant Through Somatic Embryogenesis :Case Study with Eupatorium Ayapana Vent. (Asteraceae)

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Authors

Susanta Kumar Maity
Department of Botany, Government General Degree College, Keshiary, Paschim Medinipur, PIN–721135, India

Abstract


An efficient plant regeneration protocol through somatic embryogenesis of leaf explants of a valuable medicinal plant, Eupatorium ayapana Vent., has been developed. Callus development from leaf discs were achieved within 4 weeks of callus initiation on MS medium fortified with different concentrations and combinations of BAP and sterile coconut water. Extensive growth of green nodular calli was recorded in MS medium supplemented with BAP (2 mg/l) and coconut water (20 ml/l). Transfer of differentiating calli into the medium containing lower concentrations of cytokinin and coconut water resulted into the development of cotyledonary stage somatic embryoids. Maturation of somatic embryoids and subsequent plant regeneration was accomplished in growth regulator free MS medium. Further, genetic fidelity of the micropropagated plants with that of the mother plant was assessed by isoenzyme profiles and their true-to-type nature was confirmed on the basis of monomorphic banding pattern.

Keywords


Medicinal Plant, Eupatorium Ayapana, Somatic Embryogenesis.

References