Journal of Endocrinology and Reproduction

T. Malini ¹, S. Rengarajan ¹, N. S. Venkatesh ¹, K. Balasubramanian ¹, M. M. Aruldhas ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, Taramani, Chennai 600113, IN

Abstract

Quercetin, a bioflavonoid is an inhibitor of phosphodiesterase and cAMP-independent protein kinases. The present study is attempted to delineate the effects of quercetin on basal and hCG-stimulated testosterone production by normal rat Leydig cell in vitro.

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P. Mary Julie ¹, T. Malini ¹, J. Arunakaran ¹, K. Balasubramanian ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, IN

Abstract

Effects of luteinizing hormone (LH), insulin and their combination on the regulation of prolactin (PRL) receptors on Leydig cells were studied. Leydig cells were isolated from adult male Wistar rats and measurement of PRL binding and internalization was done by incubating the cells with saturating concentration of ¹²⁵I-PRL in the presence or absence of different doses of unlabeled LH/ insulin. LH exposure caused biphasic pattern of changes in Leydig cells surface binding of ¹²⁵I-PRL was increased only by 100 and 200 ng doses of LH. Exposure of insulin at all doses registered no significant change in both cell surface bound and internalized PRL receptors. In normal cells, surface binding of ¹²⁵I-PRL was greater than the internalization at 34°C but at 4°C, surface binding remained lower than that at 34°C with negligible internalization.

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S. Singh ¹, S. Yuvaraj ¹, R. Sivakumar ¹, C. Parthasarathy ¹, T. Malini ¹, K. Balasubramanian ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, IN

Abstract

Male reproductive dysfunction is a common feature in diabetic boys and men. Defective spermatogenesis, sperm count, sperm motility, seminal fluid volume and serum testosterone, supports endocrine basis for impotence and fertility disorders. The present investigation was carried out to understand the impact of diabetes on the structure and development of epididymis during the critical period of sexual maturation in rats. Prepubertal (40 days old) and adult (120 days old) male Wistar rats were made diabetic by single injection of streptozotocin (120 mg/kg body weight for prepubertal and 65 mg/kg body weight for adult) intraperitoneally.

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S. Soudamani ¹, S. Yuvaraj ¹, S. Rengarajan ¹, R. Sivakumar ¹, T. Malini ¹, K. Balasubramanian ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, IN

Abstract

The present study was aimed at evaluating the effects of streptozotocin (STZ)-induced diabetes and insulin replacement on the androgen (AR) and estrogen receptor (ER) concentrations in the caput, corpus and cauda epididymides of prepubertal and adult Wistar rats. Prepubertal (40 days old) and adult (120 days old) male Wistar rats were made diabetic by single intraperitoneal (i.p.) injection of STZ (120 mg/kg and 60 mg/kg body weight, respectively). To one set of diabetic rats, insulin was administered daily at a dose of 3 U/100 g body weight, subcutaneously, in two equally divided doses at 8.00 AM and 6.00 PM. All the rats were killed on the 21st day of commencement of experimentation. The epididymis was dissected out and caput, corpus and cauda segments were separated, freed from spermatozoa and processed for AR and ER quantification. STZ-induced diabetes caused a marked decrease in both nuclear and cytosolic AR and ER concentrations in the caput, corpus and cauda epididymides. Insulin replacement circumvented the adverse effects of diabetes in a segment-specific manner. These findmgs emphasize the detrimental effects of diabetes and the importance of insulin in the maintenance of epididymal AR and ER concentrations in immature as well as adult rats.

Keywords

Androgen Receptor, Diabetes, Epididymis, Estrogen Receptor, Insulin.

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