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Pal, Sriparna
- Ultraviolet A (UV-A) Radiation-Induced Damage in the Skin and Vital Organs of Albino Rat: An Indirect Correlation with Melatonin
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Authors
Affiliations
1 Department of Zoology, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, IN
1 Department of Zoology, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, IN
Source
Journal of Endocrinology and Reproduction, Vol 21, No 2 (2017), Pagination: 59-66Abstract
Ultraviolet radiation is causative of generation of reactive oxygen species (ROS) in the body that significantly affects normal physiology and disturbs homeostasis. In the present study we investigated the effect of UV-A radiation exposure on the first line of defence system such as skin and vital organs such as liver, kidney and spleen of Rattus norvegicus. Adult female rats were exposed to UV-A radiation for seven days at a dose of 6.36 J/cm2 and the changes in the skin histoarchitecture, oxidative load of spleen, liver and kidney along with cellular ROS levels of splenocyte determined using DCFDA staining were recorded. UV-A exposure severely damaged the histoarchitecture of skin and reduced proliferating cell nuclear antigen (PCNA) expression. The lipid peroxidation (MDA) level in spleen, liver and kidney were increased to significant levels while the activities of the enzymatic antioxidants, SOD and catalase were significantly decreased. Significant decrease of glucose content and increase of LDH of both spleen and liver were found. Cellular damage of splenocyte was observed as evidenced by increase in percentage of intense DCFDA-stained cells in UV-A treated rats. Thus, our results clearly demonstrate that UV-A radiation exposure may have detrimental effects on the antioxidant defence system of the body, including melatonin, leading to disruption of physiology by affecting vital organs.Keywords
Kidney, Liver, Oxidative Stress; Skin, Spleen, Splenocyte, DCFDA, PCNA, UV-A Radiation.
References
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- Oxidative Damage and Apoptosis Induction by L-thyroxine in the Spleen of a Tropical Bird Perdicula asiatica: Rescue by Melatonin
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Authors
Affiliations
1 Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi - 221005, IN
1 Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi - 221005, IN
Source
Journal of Endocrinology and Reproduction, Vol 23, No 2 (2019), Pagination: 109-122Abstract
Avian thyroid gland is known to influence the immunity and reproduction in an opposite manner. In this study, we evaluated the immunostimulatory, anti-oxidative and anti-apoptotic roles of melatonin in a tropical bird, Indian jungle bush quail, Perdicula asiatica, having L-thyroxine (thyrotoxicity)-induced oxidative stress. Administration of L-thyroxine (100 μg/kg body weight) enhanced the thyroidal lipid peroxidase (LPO), with a parallel decrease in the levels of antioxidant enzyme (SOD, GPx, CAT, MDA & NO) activities. Cellular immune response (%SR) and humoral immune response (anti-KLH-IgG level) of splenocytes along with general hematological parameters (TLC, LC & HF/L ratio) decreased significantly upon L-thyroxine treatment. Further, decrease in circulatory anti-inflammatory cytokines IL-2 and TNFα suggested drastic effects of induced thyrotoxicity (elevated levels of T3 & T4) on immunity. Melatonin pre-treatment (25 μg/100g BW) during evening hours (prior to L-thyroxine treatment in the afternoon) for 30 days circumvented the deleterious effects of L-thyroxine-induced oxidative stress (level of Corticosterone) and apoptosis index of the avian spleen. Our results clearly indicate the potential of melatonin in rescuing/reducing the thyrotoxicity-induced oxidative damage to avian immunity.Keywords
Anti-KLH IgG, Apoptosis, L-Thyroxin, Melatonin, Oxidative StressReferences
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