Journal of Endocrinology and Reproduction

S. Yuvaraj ¹, C. Parthasarathy ¹, N. Srinivasan ¹, P. Govindarajulu ¹, K. Balasubramanian ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, IN

Abstract

Clinical and experimental studies have shown the adverse effects of diabetes on testicular steroidogenesis and spermatogenesis. The present study was designed to assess the effects of streptozotocin (STZ)-diabetes on Leydig cell LH receptors and steroidogenic enzymes. Diabetes was induced by single intraperitoneal injection of STZ at a dose of 65 mg/kg body weight. Induction of diabetes was confirmed by plasma insulin and glucose estimations. One set of diabetic rats was treated with insulin (3U/100g body weight/day in two equally divided doses), 3 days after STZ treatment. Control and experimental animals were killed after twenty days.

Full Text

     

S. Singh ¹, S. Yuvaraj ¹, R. Sivakumar ¹, C. Parthasarathy ¹, T. Malini ¹, K. Balasubramanian ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, IN

Abstract

Male reproductive dysfunction is a common feature in diabetic boys and men. Defective spermatogenesis, sperm count, sperm motility, seminal fluid volume and serum testosterone, supports endocrine basis for impotence and fertility disorders. The present investigation was carried out to understand the impact of diabetes on the structure and development of epididymis during the critical period of sexual maturation in rats. Prepubertal (40 days old) and adult (120 days old) male Wistar rats were made diabetic by single injection of streptozotocin (120 mg/kg body weight for prepubertal and 65 mg/kg body weight for adult) intraperitoneally.

Full Text

     

S. Soudamani ¹, S. Yuvaraj ¹, S. Rengarajan ¹, R. Sivakumar ¹, T. Malini ¹, K. Balasubramanian ¹

Affiliations
1 Department of Endocrinology, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, IN

Abstract

The present study was aimed at evaluating the effects of streptozotocin (STZ)-induced diabetes and insulin replacement on the androgen (AR) and estrogen receptor (ER) concentrations in the caput, corpus and cauda epididymides of prepubertal and adult Wistar rats. Prepubertal (40 days old) and adult (120 days old) male Wistar rats were made diabetic by single intraperitoneal (i.p.) injection of STZ (120 mg/kg and 60 mg/kg body weight, respectively). To one set of diabetic rats, insulin was administered daily at a dose of 3 U/100 g body weight, subcutaneously, in two equally divided doses at 8.00 AM and 6.00 PM. All the rats were killed on the 21st day of commencement of experimentation. The epididymis was dissected out and caput, corpus and cauda segments were separated, freed from spermatozoa and processed for AR and ER quantification. STZ-induced diabetes caused a marked decrease in both nuclear and cytosolic AR and ER concentrations in the caput, corpus and cauda epididymides. Insulin replacement circumvented the adverse effects of diabetes in a segment-specific manner. These findmgs emphasize the detrimental effects of diabetes and the importance of insulin in the maintenance of epididymal AR and ER concentrations in immature as well as adult rats.

Keywords

Androgen Receptor, Diabetes, Epididymis, Estrogen Receptor, Insulin.

Full Text