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Verma, Rakesh
- Photic Stress Modulates Cholesterol Content and Oxidative Load of Steroidogenic Organs (Adrenal, Ovary and Testis) of Indian Spotted Finch Lonchura punctulata
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Authors
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1 Pineal Research Laboratory, Department of Zoology, Centre of Advanced Study Banaras Hindu University, Varanasi – 221005, IN
1 Pineal Research Laboratory, Department of Zoology, Centre of Advanced Study Banaras Hindu University, Varanasi – 221005, IN
Source
Journal of Endocrinology and Reproduction, Vol 18, No 2 (2014), Pagination: 87-94Abstract
Steroidogenic organs such as adrenal gland and gonad are of vital importance in birds, as the adrenal gland regulates stress by modulating the levels of corticosterone, while gonads synthesize gonadal steroids that regulate breeding cycle. Cholesterol content in both adrenal and gonad reflect the physiological status of these organs for stress management and reproduction, respectively. Birds, being photosensitive, experience phototoxicity in the nature due to the rapid urbanization. If photic stress induced by constant dark (DD) and constant light (LL) influences steroidogenesis in terms of cholesterol content and total antioxidant status (TAS%) as a marker of stress was experimentally assessed in Indian spotted finch Lonchura punctulata along with measurement of peripheral corticosterone levels. DD was stressful and, hence, caused decrease of ovary / testis weight and increase of adrenal gland weight, while cholesterol content decreased significantly due to inhibition of steroidogenesis. LL stimulated gonadal function but caused decrease of cholesterol content suggesting active steroidogenesis. TAS% increased under DD and decreased under LL. The phototoxicity, whether in term of DD or LL, was stressful to Indian spotted finch as evident from increased adrenal gland weight and decreased cholesterol content that can be correlated with increased synthesis of corticosterone. It may be concluded that cholesterol content is an important marker of streroidogenic status, and extremes of photoperiod influence cholesterol content, corticosterone level and TAS%.Keywords
Cholesterol, Corticosterone, Photic-Stress, Spotted-Finch, Steroidogenic-Organs, TAS%.References
- Dominoni DM, Quetting M, Partecke J. (2013) Long-term effects of chronic light pollution on seasonal functions of European blackbirds (Turdus merula). PLOS One 8 : e85069.
- Franchimont D.,(2004) Overview of the actions of glucocorticoids on the immuneresponse: a good model to characterize new pathways of immunosuppression for new treatment strategies. Ann NY Acad Sci. 1024.1: 124–37.
- Heard RDH, Bligh EG, Cann MC, Jellnick PH, O’Donnell VJ, Rao BG, Webb JL. (1956) Biogenesis of the sterols and steroidogenesis. Recent Prog Horm Res. 12: 45.
- Navara KJ, Nelson RJ. (2007) The dark side of light at night: physiological, epidemiological, and ecological consequences. J Pineal Res. 43:215–24.
- Niekamp SR, Sutherland MA, Dahl GE, Salak-Johnson JL. (2007) Immune response of piglets to weaning stress: impact of photoperiod. J Anim Sci. 85:93-100.
- Norris DO. (2013) Vertebrate Endocrinology. Fifth Edition, USA: Elsevier Inc. USA.
- Pravosudov VV, Kitaysky AS, Saldanha CJ, Wingfield JC, Clayton NS. (2002) The effect of photoperiod on adrenocortical stress response in mountain chickadees (Poecile gambeli). Gen Comp Endocrinol 126:242-48.
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. (1999) Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radic Biol Med. 26:1231-37.
- Reiter RJ, Tan DX, Manchester LC, Qi W. (2001) Biochemical reactivity of melatonin with reactive oxygen and nitrogen species. Cell Biochem Biophys. 34: 237-56.
- Reiter, R.J. (2003). Melatonin: clinical relevance. Best Pract Res Clin Endocrinol Metabol. 17:237-85.
- Rice-Evans C, Miller NJ. (1994) Total antioxidant status in plasma and body fluids. Methods Enzymol. 234:279-93.
- Sackett GE. (1925) Modification of Bloor’s method for the determination of cholesterol in whole blood or blood serum. J Biol Chem. 64:203-05.
- Sharaw M, Dirksen T, Chaffin J. (1979) Increase in free cholesterol content of adrenal cortex after stress: radioautographic and biochemical study. Am J Anat. 156:567-75.
- Singh AK, Singh TP. (1979) Seasonal fluctuation in lipid and cholesterol content of ovary, liver, and blood serum in relation to annual sexual cycle in Heteropneustes fossilis (Bloch). Endokrinologie 73:47-54.
- Stier KS, Almasi B, Gasparini J, Piault R, Piault R, Roulin A, Jenni L. (2009) Effects of corticosterone on innate and humoral immune functions and oxidative stress in barnowl nestlings. J Exp Biol. 212:2085–91.
- Sudhakumari CC. (1995) Comparative study of pineal-adrenal inter-relationship in a nocturnal Athene brama and diurnal Perdicula asiatica bird of tropical Indian origin. PhD Thesis, Banaras Hindu University, Varanasi, India.
- Sudhakumari CC,. Haldar C. (2001) Effects of photoperiod alterations on adrenocortical, pineal and gonadal activity in nocturnal bird, Athene brama and diurnal bird, Perdicula asiatica. Zool Sci. 18:71-79.
- Wingfield JC, Maney DL, Breuner CW, Jacobs JD, Lynn S, Ramenofsky M, Richardson RD. (1998) Ecological bases of hormone–behavior interactions: “the emergency life history stage”. Am Zool. 38:191–206.
- Yadav SK, Haldar C. (2014) Experimentally induced stress, oxidative load and changes in immunity in a tropical wild bird, Perdicula asiatica: involvement of melatonin and glucocorticoid receptors. Zoology 117:261-68.
- Effect of Gonadotropins and Gonadal Steroids (17β-Estradiol and Testosterone) on Harderian Gland Porphyrin Content and Circulatory Hormones in Indian Palm Squirrel Funambulus pennanti
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Authors
Affiliations
1 Department of Zoology, Udai Pratap Autonomous Degree College, Bhojubir, Varanasi-221002, IN
2 Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi - 221005, IN
1 Department of Zoology, Udai Pratap Autonomous Degree College, Bhojubir, Varanasi-221002, IN
2 Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi - 221005, IN
Source
Journal of Endocrinology and Reproduction, Vol 18, No 1 (2014), Pagination: 1-6Abstract
Mostly studied in the hamster, the Harderian gland (HG, an extra-orbital gland) has been shown to be capable of melatonin (MEL) synthesis and to possess steroid receptor binding sites. Sexual dimorphism and ability to respond to steroid hormones have been reported for HG of some temperate zone mammals. However, to date there is no report on functional relation of HG with pineal, pituitary and gonad in any rodent. Hence, we tested the effect of gonadotropins [FSH (10μg) + LH (10μg)] and steroids [testosterone (100μg) and 17-β estradiol (50μg)] on HG function in relation to the activities of the pineal gland and gonad in both sexes of Indian palm squirrel Funambulus pennanti during the sexually quiescent phase. In general, squirrels treated with gonadotropins and gonadal steroids showed a significant increase in gonadal weight and decrease in pineal weight and plasma melatonin level in LH- and testosterone-treated groups only. There was no change in the weight of HG on FSH treatment. Porphyrin content of HG decreased after testosterone and estradiol treatment. Pineal gland weight invariably decreased after treatment with gonadotropins and testosterone. Plasma testosterone and estradiol levels increased but melatonin level decreased in all treated groups. Our data suggest that in this tropical rodent gonadotropins have an indirect effect on HG and that gonadal steroids have significant effect on HG through the influence on gonadal and pineal functions.Keywords
Gonadotropins, Harderian Gland, Melatonin, Pineal, Rodent.- Electron Microscopic Study of Harderian Gland of Tropical Bird Indian Jungle Bush Quail Perdicula asiatica
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Authors
Affiliations
1 Department of Zoology, Udai Pratap Autonomous Degree College, Bhojubir, Varanasi-221002, IN
2 Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221005, IN
1 Department of Zoology, Udai Pratap Autonomous Degree College, Bhojubir, Varanasi-221002, IN
2 Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221005, IN
Source
Journal of Endocrinology and Reproduction, Vol 18, No 1 (2014), Pagination: 41-46Abstract
The structure of Harderian gland (HG) of tropical avian species has never been studied in detail. In the present study we investigated the structure of HG of a t ropical avian species adopting light- and transmission electron microscopy. HG from both male and female adult birds, P. asiatica (N=5 each sex) were studied from light microscopic (hematoxylin-eosin staining) and transmission electron microscopic perspectives. The tubulo-alveolar HG of P. asiatica did not reveal any sexual dimorphism as reported in other birds. The HG of this bird is encapsulated by thick connective tissue consisting of collagen fibers, nerve fibers and blood vessels. HG lobules are lined with columnar epithelial cells. Plasma cells are found in interlobular spaces. The columnar epithelial cells are secretory in nature and have both rough and smooth endoplasmic reticula surrounding the nucleus when observed in transmission electron microscope. There is active Golgi apparatus with dilated cisternae. Mitochondria are well developed, with elongated cristae, and are scattered throughout the cytoplasm along with abundant free ribosomes. Lipid droplets are present in the cytoplasm along with two types of materials, electron-luscent and electron-dense. We could trace the various stages of synthesis of the secretory material for merocrine release. Thus, the light microscopic and ultrastructural organization of HG of P. asiatica have been elucidated.Keywords
Bird, Indian Jungle Bush Quail, Harderian Gland, Secretory Acini.- Ultraviolet A (UV-A) Radiation-Induced Damage in the Skin and Vital Organs of Albino Rat: An Indirect Correlation with Melatonin
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Authors
Affiliations
1 Department of Zoology, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, IN
1 Department of Zoology, Banaras Hindu University, Varanasi – 221005, Uttar Pradesh, IN
Source
Journal of Endocrinology and Reproduction, Vol 21, No 2 (2017), Pagination: 59-66Abstract
Ultraviolet radiation is causative of generation of reactive oxygen species (ROS) in the body that significantly affects normal physiology and disturbs homeostasis. In the present study we investigated the effect of UV-A radiation exposure on the first line of defence system such as skin and vital organs such as liver, kidney and spleen of Rattus norvegicus. Adult female rats were exposed to UV-A radiation for seven days at a dose of 6.36 J/cm2 and the changes in the skin histoarchitecture, oxidative load of spleen, liver and kidney along with cellular ROS levels of splenocyte determined using DCFDA staining were recorded. UV-A exposure severely damaged the histoarchitecture of skin and reduced proliferating cell nuclear antigen (PCNA) expression. The lipid peroxidation (MDA) level in spleen, liver and kidney were increased to significant levels while the activities of the enzymatic antioxidants, SOD and catalase were significantly decreased. Significant decrease of glucose content and increase of LDH of both spleen and liver were found. Cellular damage of splenocyte was observed as evidenced by increase in percentage of intense DCFDA-stained cells in UV-A treated rats. Thus, our results clearly demonstrate that UV-A radiation exposure may have detrimental effects on the antioxidant defence system of the body, including melatonin, leading to disruption of physiology by affecting vital organs.Keywords
Kidney, Liver, Oxidative Stress; Skin, Spleen, Splenocyte, DCFDA, PCNA, UV-A Radiation.References
- Valencia A, Rajadurai A, Carle AB, Kochevar IE. 7-Dehydrocholesterol enhances ultraviolet A-induced oxidative stress in keratinocytes: roles of NADPH oxidase, mitochondria and lipid rafts. Free Radic Biol Med. 2006; 41: 1704–1718. https:// doi.org/10.1016/j.freeradbiomed.2006.09.006 PMid:17145559 PMCid:PMC1880892
- Douki T, Reynaud-Angelin, A, Cade, J, Sage E. Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation. Biochemistry. 2003; 42: 9221–9226. https://doi.org/10.1021/bi034593c PMid:12885257
- Halliday, G. M. Inflammation, gene mutation and photoimmunosuppression in response to UVR-induced oxidative damage contributes to photocarcinogenesis. Mutat. Res. 2005; 571: 107–120. https://doi.org/10.1016/j.mrfmmm.2004.09.013 PMid:15748642
- Goswami, S, Haldar, C. Melatonin pre-treatment alleviates UVA radiation induced oxidative stress and apoptosis in the skin of a diurnal tropical rodent Funambulus pennantii. J Nucl Med Radiat Ther. 2016; 8: 318.
- Verma R, Haldar C. Photoperiodic modulation of thyroid hormone receptor (TR-α), deiodinase-2 (Dio-2) and glucose transporters (GLUT 1 and GLUT 4) expression in testis of adult golden hamster, Mesocricetus auratus. J Photochem Photobiol B. 2016; 165: 351–358. https://doi.org/10.1016/j.jphotobiol.2016.10.036 PMid:27838488
- Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem. 1976; 72: 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
- Vishwas DK, Mukherjee A, Haldar C, Dash D, and Nayak MK. Improvement of oxidative stress and immunity by melatonin: an age dependent study in golden hamster. Exp Gerontol. 2013; 48: 168–182. https://doi.org/10.1016/j.exger.2012.11.012 https://doi.org/10.1016/j.exger.2013.05.052 PMid:23220117
- Lijun L, Xuemei L, Yaping G, EnboM. Activity of the enzymes of the antioxidative system in cadmium-treated Oxya chinensi (Orthoptera Acridoidae). Environ Toxicol Phar. 2005; 20: 412–416. https://doi.org/10.1016/j.etap.2005.04.001 PMid:21783620
- McMillan TJ, Leatherman E, Ridley A, Shorrocks J, Tobi SE, Whiteside JR. Cellular effects of long wavelength UV light (UVA) in mammalian cells. J Pharm Pharmacol. 2008; 60: 969– 976. https://doi.org/10.1211/jpp.60.8.0004 PMid:18644190
- Hasei K, Ichihashi M. Solar urticaria: determinations of action and inhibition spectra Arch. Arch of Dermatol. 1982; 118: 346–350. https://doi.org/10.1001/archderm.118.5.346 https://doi.org/10.1001/archderm.1982.01650170060026
- Ortel B, Tanew A, Wolff K, Honisgman H. Polymorphous light eruption: action spectrum and photoprotection. J Am Acad Dermatol. 1986; 14: 748–753. https://doi.org/10.1016/S0190-9622(86)70088-1
- Garland CF, Garland FC, Gorham EC. Epidemiologic evidence for different roles of ultraviolet A and B radiation in melanoma mortality rates. Ann Epidemiol (AEP). 2003; 13: 395–404. https://doi.org/10.1016/S1047-2797(02)00461-1
- Agar NS, Halliday GM, Barnetson RStC, et al. The basal layer in human squamous tumors harbors more UVA than UVB fingerprint mutations: a role for UVA in human skin carcinogenesis. Proc Natl Acad Sci USA. 2004; 101: 4954–4959. https://doi.org/10.1073/pnas.0401141101 PMid:15041750 PMCid:PMC387355