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- N. S. Venkatesh
- M. Kathiresh Kumar
- S. Sambavi
- N. Kalpana
- A. M. Sashi John
- J. Anbalagan
- M. M. Aruldhas
- S. Sitta-Djody
- H. M. Balaganes
- N. A. Jayavelan
- M. S. Holi
- N. Srinivasan
- M. Balaganesh
- E. Thirupavai
- S. Veni
- S. Sitta Djody
- V. Sambavi
- S. Suganya
- P. Rajesh
- E. Suthagar
- A. K. Navin
- N. Shobana
- B. Ravi Sankar
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Ilangovan, R.
- Transient Neonatal-Onset Hypothyroidism Boosts Estradiol Synthesis in the Testis of Adult Wistar Rat
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Authors
N. S. Venkatesh
1,
M. Kathiresh Kumar
1,
S. Sambavi
1,
N. Kalpana
1,
A. M. Sashi John
1,
J. Anbalagan
1,
R. Ilangovan
1,
M. M. Aruldhas
1
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai – 600 113, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai – 600 113, IN
Source
Journal of Endocrinology and Reproduction, Vol 18, No 2 (2014), Pagination: 95-110Abstract
Transient neonatal hypothyroidism is known to boost Sertoli cell (SC), Leydig cell (LC) and germ cell (GC) number at adult age. Existing reports attribute decreased steroidogenic potential of LCs to unaltered/decreased serum testosterone in such rats, despite a boost in the cell number. However, these studies have ignored the status of estradiol in such conditions. In this present study, we tested the hypothesis "transient neonatal-onset hypothyroidism may lead to a temporal shift in adult rat testicular steroidogenesis towards estradiol production". Hypothyroidism was induced in neonates by providing methimazole (MMI) in drinking water (0.05%) to lactating mothers and pups for a transient period from postnatal day 1 (PND 1) to PND 14 or from PND 1 to PND 29. After the experimental period, the pups were provided drinking water free of MMI and sacrificed on PND 91. Coeval rats without MMI exposure served as control. Radioimmunoassay revealed decreased serum titres of luteinizing hormone (LH), follicle stimulating hormone (FSH), growth hormone (GH) and prolactin (PRL); while serum testosterone remained unaltered, its level in testicular interstitial fluid (TIF) decreased. Between the two major metabolites of testosterone, 5α-dihydrotestosterone (DHT) concentration decreased in serum and TIF, whereas estradiol recorded a significant increase in both. Transient neonatal-onset hypothyroidism decreased 3β-hydroxy steroid dehydrogenase (3β-HSD) in LCs but augmented 17β-HSD activity. Radio-receptor assay revealed decreased concentration of LHR and ER in LCs of hypothyroid groups, whereas AR and PRLR increased. While aromatase activity decreased in LCs, it increased along with FSHR in SCs, when compared to control rats. The changes in LHR and FSHR levels and aromatase were consistent with the expression level of the respective genes. The present study supports the proposed hypothesis and suggests that transient neonatal-onset hypothyroidism-induced boost in estradiol in adult rat testis may be due to augmented expression and activity of aromatase, and FSHR content in SCs of these rats.Keywords
Leydig Cells, Sertoli Cells, LHR, FSHR, Hypothyroidism.
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- Studies on Certain Serum Biochemical Profiles and Bone Mineral Density in Normal and Osteoporotic Men
Abstract Views :151 |
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Authors
R. Ilangovan
1,
S. Sitta-Djody
1,
H. M. Balaganes
1,
N. A. Jayavelan
2,
M. S. Holi
2,
N. Srinivasan
1
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medicai Sciences, Chennai 600113, IN
2 Institute Hospital, Biomedical Engineering Division, IIT Madras, Chennai 600 036, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medicai Sciences, Chennai 600113, IN
2 Institute Hospital, Biomedical Engineering Division, IIT Madras, Chennai 600 036, IN
Source
Journal of Endocrinology and Reproduction, Vol 7, No 1&2 (2003), Pagination: 40-41Abstract
Osteoporosis is characterized by impairment of bone mass and deterioration of bone microscopic structure, resulting in increased bone fragility and susceptibility to fracture. Osteoporosis is considered as a postmenopausal woman's disease; however, recent studies indicate that the effects of this disease in men are at least as severe as in women. Insulin-like growth factors (IGFs) and its binding proteins (IGFBPs) are known to influence the bone physiology.- Regulation of Rat Bone Marrow Stem Cells Differentiation into Osteoblasts and Mineralization by Estrogen
Abstract Views :167 |
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Authors
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, IN
Source
Journal of Endocrinology and Reproduction, Vol 7, No 1&2 (2003), Pagination: 43-43Abstract
The age-related defects in osteoblast number and function may be due to quantitative and qualitative stem cell defects. Early commitment and conversion of bone marrow stem cells (BMSCs) towards osteoblastic lineage is a critical step in the process of bone formation and bone remodeling, which is regulated by various systemic hormones and local factors. There are a few studies that analyze whether changes in the functional characteristics of osteoblastic progenitor cells, the BMSCs.- Role of RhTGF-β1 on Differentiation and Mineralization of Rat Bone Marrow Stem Cells
Abstract Views :177 |
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Authors
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, IN
Source
Journal of Endocrinology and Reproduction, Vol 7, No 1&2 (2003), Pagination: 72-72Abstract
Bone marrow is a complex tissue, which contains stem cells for haematopoietic and mesenchymal lineage. Among various growth factors that regulate bone remodeling, TGF-bl is the major growth fator, which is synthesized and stored in bone in large amount. But, the exact role of TGF-bl on the differentiation and mineralization of bone marrow stem cells (BMSCs) is still not clear. Hence, the present study is designed to delineate the role of rhTGF-bl on the differentiation and mineralization of rat BMSC in vitro. Bone marrow stem cells were isolated from the femur bones of healthy rats and cultured with osteogenic medium (OM).- Role of RhIGF-I on Differentiation and Mineralization of Rat Bone Marrow Stem Cells
Abstract Views :173 |
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Authors
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, IN
Source
Journal of Endocrinology and Reproduction, Vol 7, No 1&2 (2003), Pagination: 76-76Abstract
Bone marrow stem cells (BMSC) develop into pre-osteoblast, mature into osteoblast and attain sensitivity as osteocytes. Among the potential regulators of bone formation, insulin like growth factor-I (IGF-I) occupies an important position because of its ability to stimulate osteoblast proliferation and differentiation. Although, it is well known that osteoblasts are important target cell types, little is known about the effect of rhIGF-I on osteoprogenitors.- Effect of 17β-Estradiol on IGFBP-3 Production and Differentiation of SAOS-2 Cells in Vitro
Abstract Views :193 |
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Authors
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, IN
Source
Journal of Endocrinology and Reproduction, Vol 7, No 1&2 (2003), Pagination: 81-81Abstract
Bone formation is a dynamic process, which is regulated by various hormones and local growth factors. Numerous clinical and experimental studies have shown that estrogen deficiency delays the maturation of the skeleton and interferes with the normal growth of the bones.- Gestational Exposure to Di(2-Ethylhexyl)Phthalate Modifies the Expression Pattern of Genes Controlling Thyroid Hormone Biosynthesis in Puberal Rat Progeny
Abstract Views :268 |
PDF Views:2
Authors
V. Sambavi
1,
M. M. Aruldhas
1,
S. Suganya
1,
P. Rajesh
1,
E. Suthagar
1,
A. K. Navin
1,
N. Shobana
1,
B. Ravi Sankar
1,
R. Ilangovan
1
Affiliations
1 Department of Endocrinology, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai - 600113, Tamil Nadu, IN
1 Department of Endocrinology, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai - 600113, Tamil Nadu, IN
Source
Journal of Endocrinology and Reproduction, Vol 20, No 2 (2016), Pagination: 92-101Abstract
Di(2-ethylhexyl) phthalate (DEHP), a plasticiser, is known to disrupt thyroid functions but the underlying molecular mechanism remains obscure. The present study was conducted testing the hypothesis that gestational exposure to DEHP may modify the expression of specific genes controlling biosynthesis and action of thyroid hormones in the male progeny at puberal age. Pregnant rats were administered with DEHP [1, 10 and 100 mg (in olive oil)/Kgb.wt./day] from embryonic day 9 to 21 through oral route. The pups were sacrificed on post-natal day 60. Enzyme Immuno Assay (EIA) revealed a dose-dependent decrease in serum 3,5,3’ triiodothyronine (T3) and L-thyroxine (T4) titres in DEHP-treated rats. Real-time RT-PCR and western blot analyses of thyroidal genes revealed decreased expression level of sodium/iodide symporter (Nis) and thyroid hormone receptor α (Trα), whereas the expression of thyroid stimulating hormone receptor (Tshr), thyroid hormone receptor β (Trβ) and pendrin (Pds) increased. While western blot detection showed decreased expression level of thyroperoxidase (Tpo), RTPCR data pointed out augmented expression. Western blot detection of transcriptional factors showed decreased expression levels of fork-headbox e1 (Foxe1) and haematopoietically expressed homeobox (Hhex), whereas thyroid transcription factor-1 (Ttf-1) and paired-box domain 8 (Pax8) increased. Our study demonstrates, for the first time, that gestational exposure to DEHPaffects the expression of genes controlling thyroid hormone synthesis in puberal rat progeny, and the hypothyroid state in these rats may be linked to decreased expression of Nis, Tpo, Foxe1 and Hhex.Keywords
Pendrin, Sodium/Iodide Symporter, Thyroperoxidase, Thyrotrophin Receptor, Haematopoietically Expressed Homeobox.References
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