Journal of Endocrinology and Reproduction

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The Daily Expression Profile of Neuropeptides (gnih, gnrh3, kiss1 and kiss2):A Study of Possible Interaction in the Brain of Zebrafish (Danio rerio)


Affiliations
1 Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
2 Distributed Information Sub-Centre, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
3 Department of Biotechnology, Gauhati University, Guwahati - 781014, Assam, India
4 Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
     

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Involvement of neuropeptides in the reproduction of fish (seasonal/regular) is known. The daily rhythmicity and their possible interaction of four major neuropeptides namely gnih, gonadotropin-inhibitory hormone; gnrh, gonadotropinreleasing hormone; kiss1/2, kisspeptin 1/2; is not known to any fish. Our present study on the whole brain of zebrafish (Danio rerio) aimed at the daily rhythmicity of the mRNA expression of these four neuropeptides in a 12 h light/12 h dark photoperiod (LD). Only kiss2 in its expression gives a rhythmicity but other three peptides are not rhythmic. Moreover, the expression of gnih is 10-fold lower than gnrh3. Our STRING network analysis suggests kiss2 act as the mediator to communicate with gnih, gnrh3, and kiss1. Our present finding is indicating the important role of kiss2 in mediating the reproductive signal and may play a central role in the synchronization of the environmental signal and reproductive periodicity.

Keywords

gnih, gnrh3, kiss1, kiss2, Protein-Protein Interaction, Zebrafish.
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  • Reed MJB. (2010). Guidance on the housing and care of zebrafish Danio rerio. In: of RSftP, Animals Ct, editors. SG Research Animals Department, RSPCA, West Sussex, UK.

  • Carnevali O, Gioacchini G, Maradonna F, Olivotto I, Migliarini B. (2011). Melatonin induces follicle maturation in Danio rerio. PloS one. 6:e19978. PMid:21647435 PMCid:PMC3102064.Retrieved from: https://doi.org/10.1371/journal.pone.0019978

  • Dardente H, Birnie M, Lincoln GA, Hazlerigg DG. (2008).RFamide-related peptide and its cognate receptor in the sheep: cDNA cloning, mRNA distribution in the hypothalamus and the effect of photoperiod.PMid:18752651. Journal of neuroendocrinology. 20: 1252–1259. Retrieved from: https://doi.org/10.1111/j.1365-2826.2008.01784.x

  • Di Rosa V, López-Olmeda JF, Burguillo A, Frigato E, Bertolucci C, Piferrer F, Sánchez-Vázquez FJ. (2016). Daily rhythms of the expression of key genes involved in steroidogenesis and gonadal function in zebrafish. PloS one. 11. Retrieved from: https://doi.org/10.1371/journal.pone.0157716

  • Gopurappilly R, Ogawa S, Parhar IS. (2013). Functional significance of GnRH and kisspeptin, and their cognate receptors in teleost reproduction. Frontiers in endocrinology. 4.Retrieved from: https://doi.org/10.3389/fendo.2013.00024

  • Johnson MA, Tsutsui K, Fraley GS. (2007). Rat RFamide-related peptide-3 stimulates GH secretion, inhibits LH secretion, and has variable effects on sex behavior in the adult male rat. Hormones and behaviour. 51: 171–180.PMid:17113584 PMCid:PMC1831848. Retrieved from: https://doi.org/10.1016/j.yhbeh.2006.09.009

  • Khan ZA, Devi HS, Rajiv C, Mondal G, Devi SD, Yumnamcha T, Bharali R, Chattoraj A. (2016a). Clock system in fish: a phenomenon of “orchestrate” or “master-slave”? In: Haldar C, Gupta S, Goswami S, editors.Updates on integrative physiology and comparative endocrinology.Varanasi, India: BHU Press; p. 329–341.

  • Khan ZA, Yumnamcha T, Rajiv C, Sanjita Devi H, Mondal G, Devi SD, Bharali R, Chattoraj A. (2016b). Melatonin biosynthesizing enzyme genes and clock genes in ovary and whole brain of zebrafish (Danio rerio): Differential expression and a possible interplay. Gen Comp Endocrinol.233: 16-31. PMid:27179881. Retrieved from: https://doi.org/10.1016/j.ygcen.2016.05.014

  • Kriegsfeld LJ, Mei DF, Bentley GE, Ubuka T, Mason AO, Inoue K, Ukena K, Tsutsui K, Silver R. (2006).Identification and characterization of a gonadotropin-inhibitory system in the brains of mammals. Proceedings of the National Academy of Sciences of the United States of America. 103: 2410– 2415. PMid:16467147 PMCid:PMC1413747.Retrieved from: https://doi.org/10.1073/pnas.0511003103

  • Lee YR, Tsunekawa K, Moon MJ, Um HN, Hwang JI, Osugi T, Otaki N, Sunakawa Y, Kim K, Vaudry H, Kwon HB, Seong JY, Tsutsui K. (2009). Molecular evolution of multiple forms of kisspeptins and GPR54 receptors in vertebrates.Endocrinology. 150: 2837–2846.PMid:19164475. Retrieved from: https://doi.org/10.1210/en.2008-1679

  • Maitra SK, Chattoraj A, Mukherjee S, Moniruzzaman M.(2013). Melatonin: a potent candidate in the regulation of fish oocyte growth and maturation. General and Comparative Endocrinology. 181: 215–222.PMid:23046602. Retrieved from: https://doi.org/10.1016/j.ygcen.2012.09.015

  • Millar RP, Lu ZL, Pawson AJ, Flanagan CA, Morgan K, Maudsley SR. (2004). Gonadotropin-releasing hormone receptors.Endocrine Reviews. 25: 235-275.PMid:15082521.Retrieved from: https://doi.org/10.1210/er.2003-0002

  • Ogawa S, Ng KW, Ramadasan PN, Nathan FM, Parhar IS.(2012). Habenular Kiss1 neurons modulate the serotonergic system in the brain of zebrafish.Endocrinology. 153: 2398–2407.PMid:22454151. Retrieved from: https://doi.org/10.1210/en.2012-1062

  • Pasquier J, Lafont AG, Rousseau K, Querat B, Chemineau P, Dufour S. (2014). Looking for the bird Kiss: evolutionary scenario in sauropsids. BMC Evolutionary Biology. 14: 30. PMid:24552453 PMCid:PMC4015844.Retrieved from: https://doi.org/10.1186/1471-2148-14-30

  • Paullada-Salmeron JA, Cowan M, Aliaga-Guerrero M, Morano F, Zanuy S, Munoz-Cueto JA. (2016).Gonadotropin inhibitory hormone down-regulates the brain-pituitary reproductive axis of male european sea bass (dicentrarchus labrax). Biology of Reproduction. 94: 121.PMid:26984999. Retrieved from: https://doi.org/10.1095/ biolreprod.116.139022

  • Plant TM. (2015). 60 Years of Neuroendocrinology: The hypothalamo-pituitary-gonadal axis. The Journal of endocrinology.226: T41–54. PMid:25901041 PMCid:PMC4498991.Retrieved from: https://doi.org/10.1530/JOE-15-0113

  • Popa SM, Clifton DK, Steiner RA. (2008).The role of kisspeptins and GPR54 in the neuroendocrine regulation of reproduction. Annual Review of Physiology. 70: 213–238.PMid:17988212. Retrieved from: https://doi.org/10.1146/ annurev.physiol.70.113006.100540

  • Portaluppi F, Smolensky MH, Touitou Y. (2010). Ethics and methods for biological rhythm research on animals and human beings. Chronobiology International. 27: 1911–1929.PMid:20969531. Retrieved from: https://doi.org/10.3109/0 7420528.2010.516381

  • Rajiv C, Sanjita Devi H, Mondal G, Devi SD, Khan ZA, Yumnamcha T, Bharali R, Chattoraj A. (2016a). Cloning, phylogenetic analysis and tissue distribution of melatonin bio-synthesizing enzyme genes (Tph1, Aanat1, Aanat2 and Hiomt) in a tropical carp, Catla catla. Biological Rhythm Research: 1–16. Retrieved from: https://doi.org/10.1080/0929 1016.2016.1263019

  • Rajiv C, Sanjita Devi H, Mondal G, Devi SD, Khan ZA, Yumnamcha T, Bharali R, Chattoraj A. (2016b). Daily and seasonal expression profile of serum melatonin and its biosynthesizing enzyme genes (tph1, aanat1, aanat2, and hiomt) in pineal organ and retina: A study under natural environmental conditions in a tropical Carp, Catla catla.Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 325: 688–700. PMid:28198154. Retrieved from: https://doi.org/10.1002/jez.2061

  • Refinetti R, Lissen GC, Halberg F. (2007). Procedures for numerical analysis of circadian rhythms. Biological Rhythm Research. 38: 275–325. PMid:23710111 PMCid:PMC3663600. Retrieved from: https://doi.org/10.1080/09291010600903692

  • Roa J, Aguilar E, Dieguez C, Pinilla L, Tena-Sempere M(2008). New frontiers in kisspeptin/GPR54 physiology as fundamental gatekeepers of reproductive function.Frontiers in Neuroendocrinology. 29: 48–69.PMid:17870152.Retrieved from: https://doi.org/10.1016/j.yfrne.2007.07.002

  • Devi HS, Rajiv C, Mondal G, Khan ZA, Devi SD, Yumnamcha T, Bharali R, Chattoraj A. (2016a). Melatonin bio-synthesizing enzyme genes (Tph1, Aanat1, Aanat2 and Hiomt) and their temporal pattern of expression in brain and gut of a Tropical Carp in natural environmental conditions. Cogent Biology: 1230337.

  • Devi HS, Rajiv C, Khan ZA, Mondal G, Devi SD, Yumnamcha T, Bharali R, Chattoraj A. (2016b). Melatonin bio-synthesizing machinery in fish: a current knowledge with a special emphasis on tropical carp. Single Cell Biology.5: 1–3. Retrieved from: https://doi.org/10.4172/21689431.1000153

  • Servili A, Le Page Y, Leprince J, Caraty A, Escobar S, Parhar IS, Seong JY, Vaudry H, Kah O. (2011).Organization of two independent kisspeptin systems derived from evolutionaryancient kiss genes in the brain of zebrafish.Endocrinology.152: 1527–1540.PMid:21325050.Retrieved from: https://doi.org/10.1210/en.2010-0948

  • Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T(2003).Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Research.13: 2498–2504. PMid:14597658 PMCid:PMC403769. Retrieved from: https://doi.org/10.1101/gr.1239303

  • Smith JT, Coolen LM, Kriegsfeld LJ, Sari IP, Jaafarzadehshirazi MR, Maltby M, Bateman K, Goodman RL, Tilbrook AJ, Ubuka T, Bentley GE, Clarke IJ, Lehman MN(2008). Variation in kisspeptin and RFamide-Related Peptide (RFRP) expression and terminal connections to gonadotropin-releasing hormone neurons in the brain: a novel medium for seasonal breeding in the sheep.Endocrinology. 149: 5770– 5782. PMid:18617612 PMCid:PMC2584593. Retrieved from: https://doi.org/10.1210/en.2008-0581

  • So WK, Kwok HF, Ge W. (2005). Zebrafish gonadotropins and their receptors: II. Cloning and characterization of zebrafish follicle-stimulating hormone and luteinizing hormone subunits--their spatial-temporal expression patterns and receptor specificity. Biology of Reproduction. 72: 1382–1396. PMid:15728794. Retrieved from: https://doi.org/10.1095/biolreprod.104.038216

  • Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J, Simonovic M, Roth A, Santos A, Tsafou KP, Kuhn M, Bork P, Jensen LJ, von Mering C(2015).STRING v10: protein-protein interaction networks, integrated over the tree of life. PMid:25352553 PMCid:PMC4383874.Nucleic Acids Research. 43: D447–452.Retrieved from: https://doi.org/10.1093/nar/gku1003

  • Tang R, Dodd A, Lai D, McNabb WC, Love DR. (2007).Validation of zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization. Acta Biochimica et Biophysica Sinica. 39: 384–390.PMid:17492136. Retrieved from: https://doi.org/10.1111/j.1745-7270.2007.00283.x

  • Tsutsui K, Saigoh E, Ukena K, Teranishi H, Fujisawa Y, Kikuchi M, Ishii S, Sharp PJ. (2000). A novel avian hypothalamic peptide inhibiting gonadotropin release.Biochem Biophys Res Commun. 275: 661–667.PMid:10964719. Retrieved from: https://doi.org/10.1006/bbrc.2000.3350

  • Tsutsui K, Bentley GE, Bedecarrats G, Osugi T, Ubuka T, Kriegsfeld LJ. (2010). Gonadotropin-inhibitory hormone (GnIH) and its control of central and peripheral reproductive function. Frontiers in Neuroendocrinology. 31: 284–295.PMid:20211640. Retrieved from: https://doi.org/10.1016/j.yfrne.2010.03.001

  • Ubuka T, Inoue K, Fukuda Y, Mizuno T, Ukena K, Kriegsfeld LJ, Tsutsui K. (2012). Identification, expression, and physiological functions of Siberian hamster gonadotropininhibitory hormone. Endocrinology. 153: 373–385.PMid:22045661 PMCid:PMC3249677. Retrieved from: https://doi.org/10.1210/en.2011-1110

  • Westerfield M. (2000). The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio). Eugene: University of Oregon Press.

  • Yumnamcha T, Khan ZA, Rajiv C, Devi SD, Mondal G, Sanjita Devi H, Bharali R, Chattoraj A. (2017). Interaction of melatonin and gonadotropin-inhibitory hormone on the zebrafish brain-pituitary-reproductive axis. Molecular Reproduction and Development. PMid:28295807.Retrieved from: https://doi.org/10.1002/mrd.22795

  • Zhang Y, Li S, Liu Y, Lu D, Chen H, Huang X, Liu X, Meng Z, Lin H, Cheng CH. (2010). Structural diversity of the GnIH/GnIH receptor system in teleost: Its involvement in early development and the negative control of LH release.Peptides. 31: 1034–1043.PMid:20226824.Retrieved from: https://doi.org/10.1016/j.peptides.2010.03.003

  • Zmora N, Stubblefield J, Golan M, Servili A, Levavi-Sivan B, Zohar Y. (2014). The medio-basal hypothalamus as a dynamic and plastic reproduction-related kisspeptin-gnrh-pituitary center in fish. Endocrinology. 155: 1874–1886.PMid:24484170. Retrieved from: https://doi.org/10.1210/ en.2013-1894

  • Zohar Y, Mu-oz-Cueto JA, Elizur A, Kah O. (2010).Neuroendocrinology of reproduction in teleost fish.General and Comparative Endocrinology. 165: 438–455.PMid:19393655. Retrieved from: https://doi.org/10.1016/j.ygcen.2009.04.017


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  • The Daily Expression Profile of Neuropeptides (gnih, gnrh3, kiss1 and kiss2):A Study of Possible Interaction in the Brain of Zebrafish (Danio rerio)

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Authors

Zeeshan Ahmad Khan
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Rajendra Kumar Labala
Distributed Information Sub-Centre, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Gopinath Mondal
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Haobijam Sanjita Devi
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Chongtham Rajiv
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Thangal Yumnamcha
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Sijagurumayum Dharmajyoti Devi
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Rupjyoti Bharali
Department of Biotechnology, Gauhati University, Guwahati - 781014, Assam, India
Sunil S. Thorat
Distributed Information Sub-Centre, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India
Asamanja Chattoraj
Biological Rhythm Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal - 795001, Manipur, India

Abstract


Involvement of neuropeptides in the reproduction of fish (seasonal/regular) is known. The daily rhythmicity and their possible interaction of four major neuropeptides namely gnih, gonadotropin-inhibitory hormone; gnrh, gonadotropinreleasing hormone; kiss1/2, kisspeptin 1/2; is not known to any fish. Our present study on the whole brain of zebrafish (Danio rerio) aimed at the daily rhythmicity of the mRNA expression of these four neuropeptides in a 12 h light/12 h dark photoperiod (LD). Only kiss2 in its expression gives a rhythmicity but other three peptides are not rhythmic. Moreover, the expression of gnih is 10-fold lower than gnrh3. Our STRING network analysis suggests kiss2 act as the mediator to communicate with gnih, gnrh3, and kiss1. Our present finding is indicating the important role of kiss2 in mediating the reproductive signal and may play a central role in the synchronization of the environmental signal and reproductive periodicity.

Keywords


gnih, gnrh3, kiss1, kiss2, Protein-Protein Interaction, Zebrafish.

References





DOI: https://doi.org/10.18519/jer%2F2016%2Fv20%2F149827