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Simultaneous Bacillus anthracis Spores Detection via Aminated-Poly(Vinyl Chloride) Coated Piezoelectric Crystal Immunosensor


Affiliations
1 Department of Medical Genetics, Gulhane Military Medical Academy, 06018 Ankara, Turkey
2 Department of Chemistry, Ankara University, 06100 Ankara, Turkey
3 Department of Medical Microbiology, Gulhane Military Medical Academy, 06018 Ankara, Turkey
 

Bacillus anthracis spores are a potential threat to countries in the context of biodefense. We have already seen the destructiveness of the anthrax attacks in the recent past. This study presents an aminated-poly(vinyl chloride) (PVC-NH2) coated quartz crystal microbalance (QCM) immunosensor for simultaneous rapid detection of B. anthracis spores. PVC-NH2, synthesized in the laboratory, was used as an adhesive layer for monoclonal antibody immobilization on gold quartz crystal. The prepared QCM sensor was tested using a pathogen field strain of B. anthracis (GenBank number: GQ375871.1) under static addition and flow through procedures with different spore concentrations. Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM) were performed to characterize the surface of the sensor during the modification. Furthermore, a series of SEM micrographs were taken in order to investigate surface morphology and show the presence of the B. anthracis spores on the surface. It is concluded that B. anthracis spores can be accomplished by using amine functionalized polymer coated QCM sensors without requiring complicated immobilization procedures or expensive preliminary preparations.
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  • Simultaneous Bacillus anthracis Spores Detection via Aminated-Poly(Vinyl Chloride) Coated Piezoelectric Crystal Immunosensor

Abstract Views: 59  |  PDF Views: 4

Authors

Ali Oztuna
Department of Medical Genetics, Gulhane Military Medical Academy, 06018 Ankara, Turkey
Hasan Nazir
Department of Chemistry, Ankara University, 06100 Ankara, Turkey
Mehmet Baysallar
Department of Medical Microbiology, Gulhane Military Medical Academy, 06018 Ankara, Turkey

Abstract


Bacillus anthracis spores are a potential threat to countries in the context of biodefense. We have already seen the destructiveness of the anthrax attacks in the recent past. This study presents an aminated-poly(vinyl chloride) (PVC-NH2) coated quartz crystal microbalance (QCM) immunosensor for simultaneous rapid detection of B. anthracis spores. PVC-NH2, synthesized in the laboratory, was used as an adhesive layer for monoclonal antibody immobilization on gold quartz crystal. The prepared QCM sensor was tested using a pathogen field strain of B. anthracis (GenBank number: GQ375871.1) under static addition and flow through procedures with different spore concentrations. Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM) were performed to characterize the surface of the sensor during the modification. Furthermore, a series of SEM micrographs were taken in order to investigate surface morphology and show the presence of the B. anthracis spores on the surface. It is concluded that B. anthracis spores can be accomplished by using amine functionalized polymer coated QCM sensors without requiring complicated immobilization procedures or expensive preliminary preparations.