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Morphometric and Quantitative Analysis of the Intestine of Rattus rattus Infected by Strongyloides spp.


Affiliations
1 Animal Science Master’s Program, Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana
2 Animal Science Master’s Program, Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
3 Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
 

Strongyloidiasis is caused by Strogyloides spp., which occurs when the parasite reaches the intestines. It is estimated that more than 370 million people throughout the world are infected with this disease. The aim of this study was to analyze the morphometric alterations and variation in the count of goblet cells of the jejunum of sinantropic rodents infected by Strongyloides spp., and to compare with non-infected rodents. 157 rodents of the species of Rattus rattus were captured. After identifying the intestinal parasite, 20 rodents were selected. The criteria used were: animals between 20 and 25 cm (n=10), 5 females and 5 males infected by Strongyloides spp. and 5 females and 5 males (n=10) without the parasite. Sedimentation technique was used to identify the parasite. Morphometric and quantitative analysis of the intestinal wall was done through histological processing. In male and female infected rats, the followings were observed: smaller villus height, crypt depth increased, smaller villus area and a larger number of goblet cells in the crypts of the jejunum. The morphologic alterations of the jejunum wall may impair nutrient absorption process.

Keywords

Goblet Cells, Crypts, Jejunum, Sinantropic Rodents, Villi.
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  • Morphometric and Quantitative Analysis of the Intestine of Rattus rattus Infected by Strongyloides spp.

Abstract Views: 158  |  PDF Views: 34

Authors

Adriana Valeria Batista
Animal Science Master’s Program, Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana
Ranulfo Piau
Animal Science Master’s Program, Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Daniela Dib Goncalves
Animal Science Master’s Program, Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Gabriel Coelho Gimenes
Animal Science Master’s Program, Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Fabiane Antiquera Ferreira
Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Edson Geronimo
Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Wellington Henrique Bessi
Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Brenda Romite
Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Eduardo Herrera Dias
Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil
Valdeci Messa
Universidade Paranaense (UNIPAR), PracaMascarenhas de Moraes, 4282, Umuarama, Parana, Brazil

Abstract


Strongyloidiasis is caused by Strogyloides spp., which occurs when the parasite reaches the intestines. It is estimated that more than 370 million people throughout the world are infected with this disease. The aim of this study was to analyze the morphometric alterations and variation in the count of goblet cells of the jejunum of sinantropic rodents infected by Strongyloides spp., and to compare with non-infected rodents. 157 rodents of the species of Rattus rattus were captured. After identifying the intestinal parasite, 20 rodents were selected. The criteria used were: animals between 20 and 25 cm (n=10), 5 females and 5 males infected by Strongyloides spp. and 5 females and 5 males (n=10) without the parasite. Sedimentation technique was used to identify the parasite. Morphometric and quantitative analysis of the intestinal wall was done through histological processing. In male and female infected rats, the followings were observed: smaller villus height, crypt depth increased, smaller villus area and a larger number of goblet cells in the crypts of the jejunum. The morphologic alterations of the jejunum wall may impair nutrient absorption process.

Keywords


Goblet Cells, Crypts, Jejunum, Sinantropic Rodents, Villi.