International Journal of Plant Sciences

N. S. Dodiya ¹, C. L. Khatik ¹

Affiliations
1 Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan, IN

Abstract

The fresh seeds of isabgol variety RI-87 were subjected to 15, 30, 45, 60, 75, 90, 105,120 and 135 Kr doses at Co₆₀ gamma-rays and lab as well as field experiment was carried out during rabi 2002-03 and 2003-04. The effects of gamma-rays were studied on different parameters such as germination percent, seedling height, seedling dry weight and pollen fertility in M₁ generation and frequency and spectrum of chlorophyll and viable mutants, mutagenic efficiency and effectiveness in M₂ generation with employing nine doses of gamma-rays and one control. Reduction of germination, seeding height, seedling dry weight and pollon fertility in the M₁ generation was observed with increases doses of gamma-rays. In general the frequencies of chlorophyll mutations increased in linear fashion at low and medium doses and were erratic reduction at higher doses. The spectrum of chlorophyll mutants included albina, xantha, chlorina, viridis, tigrina and others. The frequency of these mutants varied with treatments. The 105 kR doses of gamma-rays produce highest frequency of viable mutants. Occurrence of major viable mutants from seedling to adult growth stages were varied viz., broad and narrow leaves, paired spikes, coxcomb spikes, ball mutants, gappy spikes, partial and complete sterile. A dose rate of 90 kR and 105 kR were most effective as well as efficient treatments.

Keywords

Plantago Ovata L., Mutations, Chlorophyll And Viable Mutants, Mutagenic Effectiveness, Mutagenic Efficiency

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Rajnish Kumar ¹, N. S. Dodiya ¹, C. L. Khatik ¹

Affiliations
1 Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan, IN

Abstract

The present investigation was carried out using 26 diverse genotypes including 3 checks. The genotypes were planted in Randomized Block Design with three replications during Rabi, 2010-2011 at Research Farm, Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, MPUAT, Udaipur. The observations were recorded on ten randomly selected plants and estimation of different variability parameters and magnitude of correlation co-efficient among different traits in Isabgol (Plantago ovata L. Forsk). Analysis of variance revealed significant differences among the genotypes for all the characters suggesting sufficient amount of variability in the experimental material under study. High GCV coupled with high heritability and expected genetic gain of a character provides good selection advantage. There is a substantial scope for improvement of number of florets per plant, total soluble sugar, number of effective tillers per plant and seed yield per plant. Selection for these characters would be effective in selection of suitable genotype for Isabgol improvement. Association study indicated that seed yield per plant was positively and significantly correlated at both genotypic and phenotypic level with number of tillers per plant and 1000 seed weight, whereas swelling factor showed positive significant correlation with seed yield per plant only at genotypic level. These associations indicated that improvement in seed yield can be achieved by improving the characters for further breeding programme.

Keywords

Isabgol, Variability, Character Association, Seed Yield

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