International Journal of Plant Protection

M. R. Taware ¹, V. M. Gholve ¹, S. S. Wagh ¹, D. P. Kuldhar ¹, D. V. Pawar ¹, A. A. Chavan ¹

Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN

Abstract

Eight culture media, eight temperature levels, nine pH levels, seven carbon and six nitrogen sources tested exhibited better growth of Alternaria carthami. Results revealed that Potato dextrose agar gave significantly highest growth (90.00 mm), followed by Potato malt agar (84.16 mm) with excellent sporulation. Temperature levels indicated that highest mean mycelial growth (85.66 mm) was recorded at 30°C followed by 25°C (83.83 mm) and 20°C (66.33 mm). However, maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent sporulation, followed by at pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively. The carbon sources exhibited varied radial mycelial growth and sporulation of the test pathogen. However, highest radial mycelial growth (86.00 mm) and excellent sporulation was recorded on glucose, followed by on maltose (82.83 mm) and starch (80.33 mm) with excellent sporulation. Nitrogen sources resulted highest radial mycelial growth (82.55 mm) and excellent sporulation on potassium nitrate, followed by on peptone (75.83 mm) with good sporulation. Least radial mycelial growth (19.00 mm) was recorded on urea with poor sporulation.

Keywords

Alternaria Carthami, Mycelial Growth, Carbon And Nitrogen Sources

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V. M. Gholve ¹, S. V. Pawar ¹, S. S. Wagh ¹

Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN

Abstract

Survey was conducted in four districts of Marathwada region of Maharashtra during Rabi 2013 to know the incidence and intensity of leaf blight disease caused by Alternaria carthami. The disease was predominant in all the districts surveyed. Highest per cent disease incidence and intensity was reported in Hingoli (42.08 and 38.46 %, respectively). This was followed by Nanded (40.07 and 36.03 %, respectively).

Keywords

Alternaria Blight, Incidence, Carthamus tinctorius, Severity Survey.

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V. M. Gholve ¹, V. R. Tatikundalwar ¹, S. S. Wagh ¹

Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN

Abstract

Brinjal or egg plant (Solanum melongena L.) is widely grown vegetable crop in India. Of the various diseases affecting brinjal, damping off caused by Pythium ultimum Trow. is one of the most destructive diseases causing several yield losses. The pathogen (P.ultimum) from naturally diseased brinjal plant showing typical symptoms of damping off was successfully isolated on the basal culture medium potato dextrose agar. The fungus P.ultimum produce nonseptate, well branched, colourless to whitish mycelium, sporangia on indeterminate sporangiophores when observed under the microscope. Pathogenicity of Pythium ultimum Trow. was proved by sick soil method in pot culture, sowing brinjal cv. HADGAON LOCAL under screen house condition. The pathogen was reisolated on PDA from artificially diseased brinjal seedling, and compared its cultural and morphological characteristics with the original fungus isolated from the naturally damping off diseased brinjal plant. Based on the typical symptoms of damping off, morphological and cultural characteristics, microscopic observations and pathogenicity test; the test pathogen was identified and confirmed as P. ultimum. Results revealed that of 13 brinjal cultivar lines, five lines viz., EPM-564, Ajay, Kranti seed, Brinjal MG and Puneri Kateri were moderately susceptible with disease incidence in the range of 11.10 to 16.66 per cent and five lines viz., Vishal, Arnav, Local Pingali, Local Kinwat, Local Hadgaon were susceptible with the disease incidence of about 22.21 to 44.44 per cent.

Keywords

Brinjal, Isolation, Pathogenicity, In vitro, Screening, Pythium ultimum.

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