International Journal of Plant Protection

P. B. Abhang ¹, A. A. Walunj ², M. V. Totawar ¹

Affiliations
1 Department of Plant Pathology, College of Agriculture, Loni, AHMEDNAGAR (M.S.), IN
2 Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, AKOLA (M.S.), IN

Abstract

Citrus canker disease is of regular occurrence on several citrus cultivars in varying degrees of incidence depending on the climatic conditions. The bacterium, Xanthomonas causes different symptoms ranging from pustules to necrotic lesions consisting of erumpent corky tissue surrounded by water soaked tissues and yellow halo on leaves, stems and fruits. Sensitivity of different antibiotics were tested against Xanthomonas axonopodis pv. citri isolates (Xac). using eight different Hi-media disc viz., HX006, HX007, HX010, HX032, HX038, HX060, HX067, HX069. Each Hi-media disc having six different antibiotics of different concentration have been tested against Xac isolates. The causal pathogen Xanthomonas axonopodis pv. citri showed sensitivity to most of the antibiotic tested. Hi-media disc HX060 having antibiotic Imepenem (10mg) and Ticarcillin (70mg) showed maximum zone of inhibition (42mm). However, no effect of antibiotics i.e. Co-Trimoxazole, Nofloxacin, Cefoxitin, Erythromycin observed against Xac.

Keywords

Xac, Himedia disc, Citrus canker, Antibiotics.

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A. A. Walunj ¹, P. B. Abhang ², Priya John ²

Affiliations
1 Department of Plant Pathology, College of Agriculture, Loni, AHMEDNAGAR (M.S.), IN
2 Department of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University, NAVSARI (GUJARAT), IN

Abstract

Aqueous suspension of conidia of Trichoderma harzianum wild strain Th-W were placed on potato dextrose agar and expose to UV irradiation for 10, 20, 30, and 40 min at 20 cm distance from which four stable mutants of T. harzianum i.e., Th-M-1, Th-M-2, Th-M-3 and Th-M-4 were obtained as it differed considerably from wild strain (Th-W) for their morphological characteristics. In vitro evaluation of mutant and wild strain of T. harzianum against three soil borne plant pathogens, Fusarium oxysporum f.sp. lycopersici, Sclerotium rolfsii and Macrophomina phaseolina by dual culture method, revealed that mutant strains overgrew all the pathogenic fungi more rapidaly than the wild strain.

Keywords

Mutant, Soil Borne Plant Pathogens, T. harzianum, UV-Irradiation.

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