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Evaluation of Antibiotics, Fungitoxicants and Botanicals against Xanthomonas oryzae pv. oryzae, A Cause of Bacterial Leaf Blight of Rice


Affiliations
1 Department of Agriculture, Jharkhand Rai University, Ranchi (Jharkhand), India
2 Department of Plant Pathology, Banda University of Agriculture and Technology, Banda (U.P.), India
3 Department of Plant Pathology, Dr. Kalam Agricultural College, Kishanganj (Bihar), India
     

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In vitro efficacy of different antibacterial compounds, were evaluated against Xanthomonas oryzae pv.oryzae(Xoo) causing bacterial leaf blight of rice. Six antibiotics viz.,Streptocycline, Streptomycin, Streptomycin sulphate, Plantomycin, Tetracycline hydrochloride and Oxytetracycline hydrochloride were evaluated for their efficacy against the growth of Xoo cultures at two levels (50 and 100ppm) of concentration except Plantomycin (500 and 1000 ppm) using inhibition zone assay method. The largest inhibition zone (25 mm) was documented in Tetracycline hydrochloride @ 100ppm, followed by 23 mm obtained in Streptomycin @ 100ppm. Five fungitoxicants comprising Copper Oxychloride 50 % WP (0.15 and 0.25%), Copper hydroxide 77 % WP (0.15 and 0.25%), Carbendazim 50 % WP (0.05 and 0.15%), Validamycin 3L (0.15 and 0.25%) and Propiconazole 25% EC (0.05 and 0.1%) were evaluated for their efficacy against growth of Xoo cultures at two levels of concentration. The maximum inhibition zone (19.34mm) was recorded in Validamycin @ 0.25% followed by 18.16mm observed in copper oxychloride @ 0.25%. Effect of combination of antibiotics and fungitoxicants were studied using a set comprising of six commercially available antibiotics viz., Streptocycline (100 ppm), Streptomycin (100 ppm), Streptomycin sulphate (100 ppm), Plantomycin (1000 ppm), Tetracycline hydrochloride (100 ppm), Oxytetracycline hydrochloride (100 ppm), and three fungitoxicants i.e.Copper oxychloride 50 % WP (0.25%), Copper hydroxide 77% WP (0.25%), Carbendazim 50 % WP (0.15%) were evaluated for their efficacy against growth of Xoo. Single concentration of each antibiotic will be evaluated with combination of three fungitoxicants separately. Maximum inhibition zone (25.8mm) was obtained in case of Streptocycline + Carbendazim which is at par with Tetracycline hydrochloride + Copper oxychloride and Streptomycin sulphate + Copper hydroxide. The cultures of Xoo were also screened with plant extracts viz., Neem leaf, Garlic bulb, Onion bulb, Ginger rhizome, Tulsi leaf at three levels (10, 20 and 30%) of concentration for their antibacterial properties against Xoo. The maximum inhibition zone (9.2 mm) was recorded in Garlic @ 30% followed by 9.14mm resulted in Tulsi @ 30 per cent.

Keywords

Antibiotics, Bacterial Leaf Blight, Fungitoxicants, Rice, Streptocycline, Xanthomonas oryzae.
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  • Adhikari, T.B., Vera Cruz, C.M., Zhang, Q., Nelson, R.J., Skinner, D.Z., Mew, T.W. and Leach, J.E. (1995).Genetic diversity of Xanthomonas oryzae pv.oryzaein Asia. Appl. & Environ. Microbiol., 61(3) : 966-971.
  • Ashrafuzzaman, H. (1987).Chemical Control of Bacterial Leaf Blight of Paddy Caused by Xanthomonas campestris pv. oryzae. In: Civerolo E.L., Collmer A., Davis R.E., Gillaspie A.G.(eds) Plant Pathogenic Bacteria. Current Plant Science and Biotechnology in Agriculture, vol 4. Springer, Dordrecht.
  • Balaraman, K. and Rajagopalan, C.K.S. (1978).Control of bacterial leaf blight of rice – assay of chemicals. Madras Agric.J., 65: 463-469.
  • Chauhan, H.L. and Vaishnav, M.U. (1980). Control of bacterial blight of rice caused by Xanthomonas oryzae. Indian J. Mycology & Plant Pathol., 10(1):77-79.
  • Delseny, M., Salses, J., Cooke, R., Sallaud, C. and Regad, F. (2001).Agricultural sustainability and intensive production practices. Nature, 418(6898):671-7.
  • Fernando, P.C. (2006).Agriculture, pesticides, food security and food safety. Environ. Sci. & Policy, 9(7-8):685-692.
  • Gnanamanickam, S.S., Priyadarsini, V.B., Narayanan, N.N., Vasudevan, P. and Kavitha, S. (1999).An overview of bacterial blight disease of rice and strategies for its management. Curr.Sci., 77(11):1435-1443.
  • Hobbs, P.R. (2001).Tillage and crop establishment in South Asian rice -wheat systems: Present practices and future options. J. Crop Production, 4(1):1-22.
  • Kagle, S., Marimuthu, T., Thayumanavan, B., Nandakumar, R. and Samiyappan, R. (2004).Antibacterial activity and induction of systemic resistance in rice by leaf extract of Datura metelagainst Rhizoctonia solaniand Xanthomona oryzae pv. oryzae. Physiological Molecular Plant Pathol., 65 : 91-100.
  • Khan, J.A., Siddiq, R., Arshad, H.M.I., Anwer, H.S., Saleem, K. and Jamil, F.F. (2012).Chemical control of bacterial leaf blight of rice caused by Xanthomonas oryzae pv.oryzae . Phytopathology, 24 : 97-100.
  • Khan, T.Z., Yasin, S.I., Muhammad, A., Shah, J.A. and Mustaq, A. (2005).Effect of different chemicals and antibiotics on bacterial leaf blight (X. o. pv. oryzae) of rice. Mycopathol., 3 : 57-59.
  • Kumar, M., Pirate, R.L. and Ninawe, B.N. (2009).Effect of botanicals, bioagents and some chemicals against Xanthomonas oryzae pv. oryzae. J. Plant Disease Sci., 4(1):60-63.
  • Mahto, B.N., Singh, R.N. and Singh, G.P. (1988).Response of rice bacterial blight (BB) pathogen in vitroto antibiotics and fungi toxicants. Internat. Rice Research Newsletter , 13(1):23.
  • Mary, C.A., Nair, S.K. and Saraswathy, P. (2001 ).Efficacy of prophylactic and curative sprayings on the control of bacterial blight of rice. Tropical Agric., 39(1):73-75.
  • Meena, C. and Gopalakrishnan, J. (2004). Efficacy of plant extracts against bacterial blight of rice. Ann. Plant Protec. Sci., 12(2):334-346.
  • Mew, T.W., Alwarez, A.M., Leach, J.E. and Swings, J. (1993). Focus on bacterial blight of rice. Plant Disease, 77(1):5-12.
  • Naqvi, S.A.H., Perveen, R., Umer, U.U.D., Malik, O., Rehman, A.U., Wazeer, S. and Majid, T. (2014).Determination of antibacterial activity of various broad spectrum antibiotics against Xanthomonas oryzae pv. oryzae, a cause of bacterial leaf blight of rice. Internat. J. Microbiol. & Mycol., 2: 12-19.
  • Narasimhan, V., Selvam, R. and Mariappan, V. (1995).In: Mariappan V(editor). Neem for the management of crop diseases. Associated Publishing Co. New Delhi, 15 –121.
  • Ou, S.H. (1985).Rice Diseases. 2nd Edn. Commonwealth Mycological Institute, UK, pp.61-96.
  • Parthasarathy, R., Indira, J., Appu, M. A. and Karthikeyan, A. (2014).To study the antagonistic organism and new chemicals against the bacterial blight pathogen (Xanthomonas oryzae pv. oryzae) of rice. Internat. J. Res. Biotechnol. & Biochem., 4 : 6-11.
  • Patel, S.J., Chauhan, H.L., Mehta, A.N. and Gohil, N.M. (2009). Management of bacterial blight of rice with chemicals, botanicals and antagonists. J. Plant Disease Sci., 4(2): 208-211.
  • Patel, S.J. (2008).Management of bacterial blight of rice. Ph.D. (Ag.) Thesis. Department of Plant Pathology, N.M. College of Agriculture, Navsari Agricultural University, Navsari – 396 450, Gujarat, 302 pp.
  • Prasad, D. Singh, R. and Deep, S. (2018). In-vitro and In-vivo Efficacy of Antibacterial Compounds against Xanthomonas oryzae pv. oryzae, A Cause of Bacterial Leaf Blight of Rice. Internat. J. Curr. Microbiol. Appl. Sci., 7(05): 2960-2969.
  • Raju, R.A. (2000).Glimpses of Rice Technology. Agro Bios., New Delhi. Rao, P.S. and Kauffman, H.E. (1977).Potential yield losses in dwarf rice varieties due to bacterial blight in India. Phytopathologische Zeitschrift, 90(3):281-284.
  • Sheehy, J.E. and Mitchell, P.L. (2011).Rice and global food security: The race between scientific discovery and catastrophe. The search for better nutrition. (p.81–90) Cambridgeshire, United Kingdom: Smith-Gordon.
  • Singh, R., Yadav, R.S. and Javeria, S. (2015).Management of bacterial leaf blight of Basmati rice caused by Xanthomonas oryzaepv. oryzae with some available antibiotics and plant products. Internat. J. Innovative & Appl. Res., 3 (11): 1- 6
  • Singh, R.S. (2009).Plant Diseases. Oxford & IBH. Pub. Co. Pvt. Ltd. pp.64-65.
  • Skamnioti, P. and Gurr, S.J. (2009). Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol., 27(3):141-50.
  • Srivastava, D.N. (1967).Epidemiology and control of BLB of rice in India. In: proceedings of asymposium in rice diseases and their control by growing resistant varieties and other measures. Japan Agric., Forestry & Fisheries Research Council, 11:18.
  • Sunder, S., Singh, R. and Dodan, D.S. (2005).Management of bacterial blight of rice with botanical extracts and non-conventional chemicals. Plant Disease Res., 20(1):12-17.
  • Swati, Kumar, A., Roy, S. P. and Kumai, P. (2015).Studies on efficacy of different chemical treatments against bacterial leaf blight of rice in Bihar. The Biobrio: An Internat. Quarterly J. Life Sci., 2: 56 – 61
  • Tandan, A.L. and Chaliganjewar, S.D. (2016).Strategies for management of bacterial blight disease of rice caused by Xanthomonas oryzae pv. oryzae: an overview. Internat. J. Advanced Res., 4: 150-162
  • Thimmegowda, P.R., Sataraddi, A., Ambika, D.S., Prasad, P.S. and Chandrashekar, M. (2012).Efficacy of antibiotics and biorational pesticides against bacterial blight of paddy. Madras Agric. J., 99(7-9):592-596.

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  • Evaluation of Antibiotics, Fungitoxicants and Botanicals against Xanthomonas oryzae pv. oryzae, A Cause of Bacterial Leaf Blight of Rice

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Authors

Sonika Deep
Department of Agriculture, Jharkhand Rai University, Ranchi (Jharkhand), India
Durga Prasad
Department of Plant Pathology, Banda University of Agriculture and Technology, Banda (U.P.), India
Subhashish Sarkhel
Department of Plant Pathology, Dr. Kalam Agricultural College, Kishanganj (Bihar), India

Abstract


In vitro efficacy of different antibacterial compounds, were evaluated against Xanthomonas oryzae pv.oryzae(Xoo) causing bacterial leaf blight of rice. Six antibiotics viz.,Streptocycline, Streptomycin, Streptomycin sulphate, Plantomycin, Tetracycline hydrochloride and Oxytetracycline hydrochloride were evaluated for their efficacy against the growth of Xoo cultures at two levels (50 and 100ppm) of concentration except Plantomycin (500 and 1000 ppm) using inhibition zone assay method. The largest inhibition zone (25 mm) was documented in Tetracycline hydrochloride @ 100ppm, followed by 23 mm obtained in Streptomycin @ 100ppm. Five fungitoxicants comprising Copper Oxychloride 50 % WP (0.15 and 0.25%), Copper hydroxide 77 % WP (0.15 and 0.25%), Carbendazim 50 % WP (0.05 and 0.15%), Validamycin 3L (0.15 and 0.25%) and Propiconazole 25% EC (0.05 and 0.1%) were evaluated for their efficacy against growth of Xoo cultures at two levels of concentration. The maximum inhibition zone (19.34mm) was recorded in Validamycin @ 0.25% followed by 18.16mm observed in copper oxychloride @ 0.25%. Effect of combination of antibiotics and fungitoxicants were studied using a set comprising of six commercially available antibiotics viz., Streptocycline (100 ppm), Streptomycin (100 ppm), Streptomycin sulphate (100 ppm), Plantomycin (1000 ppm), Tetracycline hydrochloride (100 ppm), Oxytetracycline hydrochloride (100 ppm), and three fungitoxicants i.e.Copper oxychloride 50 % WP (0.25%), Copper hydroxide 77% WP (0.25%), Carbendazim 50 % WP (0.15%) were evaluated for their efficacy against growth of Xoo. Single concentration of each antibiotic will be evaluated with combination of three fungitoxicants separately. Maximum inhibition zone (25.8mm) was obtained in case of Streptocycline + Carbendazim which is at par with Tetracycline hydrochloride + Copper oxychloride and Streptomycin sulphate + Copper hydroxide. The cultures of Xoo were also screened with plant extracts viz., Neem leaf, Garlic bulb, Onion bulb, Ginger rhizome, Tulsi leaf at three levels (10, 20 and 30%) of concentration for their antibacterial properties against Xoo. The maximum inhibition zone (9.2 mm) was recorded in Garlic @ 30% followed by 9.14mm resulted in Tulsi @ 30 per cent.

Keywords


Antibiotics, Bacterial Leaf Blight, Fungitoxicants, Rice, Streptocycline, Xanthomonas oryzae.

References