Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development of Antifungal Formulations and their Evaluation against Root Rot Disease of Mulberry


Affiliations
1 Central Sericultural Research and Training Institute, Mysore (Karnataka), India
     

   Subscribe/Renew Journal


Mulberry (Morus sp.) cultivated throughout India for rearing of silkworm (Bombyx mori L.). Root rot caused by a group of fungi is a severe threat for mulberry (Morus spp.) leaf production, especially in southern states of India due to large scale mortality and enormous crop loss. Fungi such as Fusarium solani, Fusarium oxysporum, Rhizoctonia bataticola and Botryodiplodia theobromae are frequently isolated from the infected ischolar_mains. Few control measures recommended could not sustain due to inconsistent results. In this perspective, studies were conducted to develop a broad spectrum formulation to contain the disease. Several plant products, synthetic fungicides and chemicals were screened for antifungal activities in vitro in solid and broth media using poisoned food technique. Five formulations were made using selected antifungal components and tested against ischolar_main rot disease under artificial simulation. All the formulations significantly (P<0.01) reduced wilting and rotting compared with untreated control as well as plants treated existing control measure. Highest control of wilting (88.20%) and rotting (88.05%) was showed by formulation F-1, followed by F-2 and F-4 compared with untreated control. The highly effective formulation (F-1) was further tested in the hotspot areas of Karnataka showed revival of plants with range of 67-86 per cent. This eco-friendly formulation could be used for control of ischolar_main rot disease of mulberry.

Keywords

Antifungal Formulations, Field Evaluation, Mulberry, Root Rot Disease.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Aneja, K.R. (2017). Experiments in Microbiology, New Age International Publications, New Delhi, India.
  • Beevi, D.N. and Qadri, S.M.H. (2010). Biological control of mulberry ischolar_main rot disease (Fusarium spp.) with antagonistic microorganisms. J. Biopesticides, 3(1): 90 - 92.
  • Choudhari, S.S., Solanke, N.S. and Kareppa, B.M. (2012). Integrated management of ischolar_main rot disease of mulberry caused by Fusarium solani. Multilogic Sci., 2 (2): 135-139.
  • Chowdhary, N.B., Sharma, D.D., Mala, V. R. and Qadri, S.M.H. (2011). Damaging threshold level of Macrophomina phaseolina causing charcoal ischolar_main rot disease in mulberry. Sericologia, 51 (1); 135-139
  • Mallikarjuna B., Magadum, S.B. and Gunashekar V. (2010) A survey on incidence of ischolar_main diseases of mulberry. Karnataka J. Agric. Sci.,23 (4) : 655.
  • Manmohan, M.S. and Govindaiah (2012). Efficacy of botanical extracts against Fusarium oxysporum Schlecht causing mulberry ischolar_main rot–an in vitro evaluation. Internat. J. Sci. & Nature, 3(2): 267-271
  • Muthuswami, M., Subramanian, R., Gangadhara, M., Bharathi and Gnanam, R. (2011). Induction of tolerance to ischolar_main rot disease in Indian mulberry varieties through in vitro approaches. Sericologia, 51(1): 107-117.
  • Narayanan, P., Vanitha, S., Rajalakshmi, J., Parthasarathy, S., Arunkumar, K., Nagendran, K. and Karthikeyan, G. (2015). Efficacy of bio-control agents and fungicides in management of mulberry wilt caused by Fusarium solani. J. Biological Control, 29(2): 107-114.
  • Philip, T. and Sharma, D.D. (1997). In vitro evaluation of leaf and oilcake extracts of Azadirachta indica and Pongamia glabra on mulberry ischolar_main rot pathogens. Indian J. Seric., 36(2): 150-152
  • Philip, T., Latha, J., Govindaiah, Mallikarjuna, B., Mondal, K.C. and Bajpai, A.K. (1995). Some observations on the incidence, associated microflora and control of ischolar_main rot disease of mulberry in South India. Indian J. Seric., 34(2):137-139.
  • Pratheesh Kumar, P.M., Magana, Y. and Sivaprasad, V. (2017). Biocontrol and growth promotion activity of indigenous Trichoderma isolates of mulberry gardens In: Recent Trends in Agriculture Veterinary & Life sciences, (Eds.) P.B. Reddy & Ratnakar D Bala, IMRF, India pp 11-20.
  • Quadri, S.M.H., Bevi, D.N. and Chowdary, N.B. (2005). Observations on the spatial prevalence and crop losses due to ischolar_main rot in mulberry (Morus alba L.). In: Advances in Tropical Sericulture. Dandin, S. B., Misra, R.K., Gupta, V.P., Reddy, Y. S. (Eds.). National Academy of Sericultural Sciences, Bangalore, India, pp. 147-149.
  • Rajeswari, P. and Angappan, K. (2018). Prevalence of mulberry ischolar_main rot disease in Tamil Nadu. Internat. J. Chemical Studies, 6: (3): 1573-1575.
  • Sharma, D.D., Naik, V.N., Chowdary, N.B. and Mala, V.R. (2003). Soil borne diseases of mulberry and their management - A review. Internat. J. Indust. Entomol., 7(4): 93-106.
  • Sridhar, R., Subramanian, A. and Chandramohan, N. (2000). Management of mulberry ischolar_main rot with antagonistic microorganisms. Sericologia, 40: 383-386.

Abstract Views: 209

PDF Views: 0




  • Development of Antifungal Formulations and their Evaluation against Root Rot Disease of Mulberry

Abstract Views: 209  |  PDF Views: 0

Authors

P. M. Pratheesh Kumar
Central Sericultural Research and Training Institute, Mysore (Karnataka), India

Abstract


Mulberry (Morus sp.) cultivated throughout India for rearing of silkworm (Bombyx mori L.). Root rot caused by a group of fungi is a severe threat for mulberry (Morus spp.) leaf production, especially in southern states of India due to large scale mortality and enormous crop loss. Fungi such as Fusarium solani, Fusarium oxysporum, Rhizoctonia bataticola and Botryodiplodia theobromae are frequently isolated from the infected ischolar_mains. Few control measures recommended could not sustain due to inconsistent results. In this perspective, studies were conducted to develop a broad spectrum formulation to contain the disease. Several plant products, synthetic fungicides and chemicals were screened for antifungal activities in vitro in solid and broth media using poisoned food technique. Five formulations were made using selected antifungal components and tested against ischolar_main rot disease under artificial simulation. All the formulations significantly (P<0.01) reduced wilting and rotting compared with untreated control as well as plants treated existing control measure. Highest control of wilting (88.20%) and rotting (88.05%) was showed by formulation F-1, followed by F-2 and F-4 compared with untreated control. The highly effective formulation (F-1) was further tested in the hotspot areas of Karnataka showed revival of plants with range of 67-86 per cent. This eco-friendly formulation could be used for control of ischolar_main rot disease of mulberry.

Keywords


Antifungal Formulations, Field Evaluation, Mulberry, Root Rot Disease.

References