International Journal of Plant Protection

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Status of Microflora on Bt and Non - Bt Cotton


Affiliations
1 Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahemdnagar (M.S.), India
     

   Subscribe/Renew Journal


Transgenic Bt cotton expresses Cry1Ac protein from Bacillus thuringiensis. The diversity of ectophytic and endophytic fungi and bacteria in ischolar_mains, stems and leaves from transgenic (Bt) and non transgenic (non Bt)cotton was evaluated during 30, 60 and 90 DAS to investigate possible non-target effects of genetically modified cotton on microbial communities. Total ten fungal and five bacterial organisms were isolated. This studies shows that the ischolar_mains, stems and leaves of Bt and non Bt cotton plants harboured endophytes and ectophytes. Although the no.of endophytic and ectophytic species isolated from the two types of plant did not vary much. While Bt modifications had no effect on endophytes and ectophytes and it is seen from the observations that the Bt gene had not transferred from Bt plants to associated microflora. These results represent the first evaluation of the composition of endophytic and ectophytic fungi as well as bacteria associated with transgenic cotton plants. Also detection of Bt gene in associated microflora by using Bt Express strips.

Keywords

Bt Cotton, Bacteria, Fungi, Microbial Diversity.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Anonymous (2014). Manual for cotton cultivation CICR Nagpur. WWF-India, NEW DELHI, INDIA.

  • Anonymous (2015). Monistory of textile Gov. of India. ON512 ON9392015. Indiastat.com.

  • Barnett, H.L. (1960). Illustrated genera of imperfect fungi. Begess Publishing Company, IInd Ed.1-219pp.

  • Barthalomew, J.W. and Mittewer, T. (1950). A simplified bacterial strain. Stain Technology, 25: 153.

  • Blackwood,C.B. and Buyer, J.S. (2004). Soil microbial communities associated with Bt and non-Bt corn in three soils. J. Environ. Qual., 33 : 832–836.

  • Brusetti, L., Francia, P., Bertolini, C., Pagliuca, A., Borin, S., Sorlini, C., Abruzzese, A., Sacchi, G., Viti, C., Giovannetti, L., Giuntini, E., Bazzicalupo, M. and Daffonchio, D. (2005). Bacterial communities associated with the rhizosphere of transgenic Bt176 maize (Zea mays) and its non-transgenic counterpart. Plant Soil.,266 : 11–21.

  • Castaldini, M., Turrini, A., Sbrana, C., Benedetti, A., Marchionni, M., Mocali, S., Fabiani, A., Landi, S., Santomassimo, F., Pietrangeli, B., Nuti, M.P., Miclaus, N. and Giovannetti, M. (2005). Impact of Bt corn on rhizospheric and soil eubacterial communities and on beneficial mycorrhizal symbiosis in experimental microcosms. Appl. Environ. Microbiol.,71 : 6719–6729.

  • De Souza Vieira, P.D., de Souza Motta, C.M., Lima, D., Torres, J.B., Quecine, M.C., Azevedoc, J.L. and de Oliveira, N.T. (2011). Endophytic fungi associated with transgenic and non-transgenic cotton. Mycology, 2(2) : 91–97.

  • Donegan, K.K., Schaller, D.L., Stone, J.K., Ganio, L.M., Reed, G., Hamm, P.B. and Seidler, R.J. (1996). Microbial populations, fungal species diversity and plant pathogen levels in field plots of potato plants expressing the Bacillus thuringiensis var. tenebrionis endotoxin. Transgenic Research, 5 : 25-35.

  • Fang, M., Kremer, R.J., Motavalli, P.P. and Davis, G. (2005). Bacterial diversity in rhizospheres of non-transgenic and transgenic corn. Appl. Environ. Microbiol., 71 : 4132–4136.

  • Filion, M. (2008). Do transgenic plants affect rhizobacteria populations. Microb. Biotechnol., 1 : 463–475.

  • Gebhard, F. and Smalla, K. (1999).Monitoring field releases of geneti- cally modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbiol. Ecol., 28 : 261–271.

  • Griffiths, B.S., Caul, S., Thompson, J., Birch, A.N.E., Scrimgeour, C., Andersen, M.N., Cortet, J., Messéan, A., Sausse, C., Lacroix, B. and Krogh, P.H. (2005). A comparison of soil microbial community structure, protozoa and nematodes in field plots of conventional and genetically modified maize expressing the Bacillus thuringiensis CryIAb toxin. Plant Soil., 275 : 135–146.

  • Griffiths, B.S., Caul, S., Thompson, J., Birch, A.N.E., Scrimgeour, C., Cortet, J., Foggo, A., Hackett, C.A. and Krogh, P.H. (2006). Soil microbial and faunal community responses to Bt maize and insecticide in two soils. J. Environ. Qual., 35 : 734–741.

  • Honemann, L., Zurbrugg, C. and Nentwig, W. (2008). Effects of Bt-corn decomposition on the composition of the soil meso-and macrofauna. Appl. Soil Ecol., 40 : 203–209.

  • Icoz, I. and Stotzky, G. (2008).Cry3Bb1 protein fromBacillus thuringiensis in ischolar_main exudates and biomass of transgenic corn does not persist in soil. Transgenic Res., 17 : 609–620.

  • Koskella, J. and Stotzky, G. (2002). Larvicidal toxins from Bacillus thuringiensis sub spp. kurstaki, morrisoni (strain tenebrionis) and israelensis have no microbicidal or microbiostatic activity against selected bacteria, fungi and algae in vitro. Can. J. Microbiol., 48 (3) : 262–267.

  • Kumar, R. and Sinha, R. P. (2011). Colloidal gold based dipstick strip for detection of genetically modified crops and produce. Internat. J. Pharma & Bio Sci., 2 (3) : 110-131.

  • Lilley, A.K., Bailey, M.J., Cartwright, C., Turner, S.L. and Hirsch P.R. (2006). Life in earth: The impact of GM plants on soil ecology? Trends Biotechnol., 24 : 9–14.

  • Mulder, C., Wouterse, M., Raubuch, M., Roelofs, W. and Rutgers, M. (2006). Can Transgenic maize affect soil microbial communities? PLoS Comput Biol., 2 : 128.

  • Paget, E., Lebram, M., Freyssinet, G. and Simonet, P. (1998). The fate of recombinant plant DNA in soil. Eur. J. Soil. Biol., 34 (2) : 81-88.

  • Prischl, M., Hackl, E., Pastar, M,. Pfeiffer, S. and Sessitsch, A. (2012). Genetically modified Bt maize lines containing cry3Bb1, cry1A105 or cry1Ab2 do not affect the structure and functioning of ischolar_main-associated endophyte communities. Appl. Soil Ecol.,54 : 39– 48.

  • Sarkar, B., Patra, A. K., Purakayastha, T. J. and Megharaj, M. (2009).Assessment of biological and biochemical indicators in soil under transgenic Bt and non-Bt cotton crop in a sub-tropical environment. Environ. Monitor. & Assess.,156 : 595–604.

  • Saxena, D. and Stotzky, G. (2001). Bacillus thuringiensis (Bt) toxin released from ischolar_main exudates and biomass of Bt corn has no apparent effect on earthworms, nematodes, protozoa, bacteria and fungi in soil. Soil Biology & Biochemist., 33 : 1225-1230.

  • Suryanarayanan, T. S., Venkatachalam, A. and Govinda, M. B. (2011). A comparison of endophyte assemblages in transgenic and non-transgenic cotton plant tissues. Curr. Sci., 101 : 1442-1444.

  • Tarafdar, J.C. and RathoreI, S.V. (2012). Effect of transgenic cotton on soil biological health. Appl. Biol. Res., 14(1):15–23.

  • Widmer, F., Seidler, R.J., Donegan, K.K. and Reed, G.L. (1997). Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol., 6 : 1-7.


Abstract Views: 167

PDF Views: 0




  • Status of Microflora on Bt and Non - Bt Cotton

Abstract Views: 167  |  PDF Views: 0

Authors

S. N. Ambhore
Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahemdnagar (M.S.), India
B. G. Barhate
Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahemdnagar (M.S.), India

Abstract


Transgenic Bt cotton expresses Cry1Ac protein from Bacillus thuringiensis. The diversity of ectophytic and endophytic fungi and bacteria in ischolar_mains, stems and leaves from transgenic (Bt) and non transgenic (non Bt)cotton was evaluated during 30, 60 and 90 DAS to investigate possible non-target effects of genetically modified cotton on microbial communities. Total ten fungal and five bacterial organisms were isolated. This studies shows that the ischolar_mains, stems and leaves of Bt and non Bt cotton plants harboured endophytes and ectophytes. Although the no.of endophytic and ectophytic species isolated from the two types of plant did not vary much. While Bt modifications had no effect on endophytes and ectophytes and it is seen from the observations that the Bt gene had not transferred from Bt plants to associated microflora. These results represent the first evaluation of the composition of endophytic and ectophytic fungi as well as bacteria associated with transgenic cotton plants. Also detection of Bt gene in associated microflora by using Bt Express strips.

Keywords


Bt Cotton, Bacteria, Fungi, Microbial Diversity.

References