International Journal of Plant Protection

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In Vitro Efficacy of Beauveria bassiana (Balsamo) Vuill., Metarhizium anisopliae (Metsch.) Sorokin and Lecanicillium lecanii (Zimmerman) against Maconellicoccus hirsutus (Green) and Ferrisia virgata (Cockerell) (Hemiptera:Pseudococcidae)


Affiliations
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
     

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Maconellicoccus hirsutus (Green) and Ferrisia virgata (Cockerell) were mass cultured in insectary using hibiscus plants, potato sprouts and the different stages were maintained to conduct bioassay. Entomopathogenic fungi Beauveria bassiana, Metarhizium anispoliae, Lecanicillium lecanii was sub-cultured on SDAY. Spore suspensions of concentration (1x 108conidia/ml) of seven isolates (BbBp1, BbGW1, BbBM1, MaBp1, MaBm1, LlBm1, LlMo1) were prepared from the 15 day old culture of the fungi. A preliminary study on B. bassiana against Maconellicoccus hirsutus (Green) and Ferrisia virgata (Cockerell) female adults was done. untreated (sterile water) were used as controls.Mortality of pink mealy bug (Maconellicoccus hirsutus (Green)) adults under laboratory condition was recorded at different intervals where entomopathogens (% mortality after 9th day) like LlMo1 (93.33%), BbGW1 (80.00%) and MaBm1 (63.33%). The mortality of guava mealy bug [Ferrisia virgata (Cockerell)] adults under laboratory condition was recorded at different intervals where entomopathogens (% mortality after 9th day) like LlMo1 (96.55%), BbBp1 (86.21%) and MaBp1 (65.52%).

Keywords

Beauveria bassiana, Pink Mealy Bug, Guava Mealy Bug, Spore, Mortality.
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  • Abbas G., Arif, M.J., Saeed, S. and Karar, H. (2009). A new invasive species of genus Phenacoccus Cockerell attacking cotton in Pakistan. Internat. J.Agric.& Biol.,11 (1): 54–58.

  • Anand, R., Prasad, B. and Tiwary, B.N. (2008). Relative susceptibility of Spodoptera litura pupae to selected entomopathogenic fungi. Biocontrol, 54 (1) : 85-92.

  • Anand, R. and Tiwary, B.N. (2009). Pathogenicity of entomopathogenic fungi to eggs and larvae of Spodoptera litura, the common cutworm. Biocontrol Sci. Technol., 19 (9): 919-929.

  • Ben-Zeev, Zelig, Y., Bitton, S. and Kenneth, R.G. (1994). The entomopathology of Israel and their orthropod hosts. Phytoparasitica, 16 : 247-257.

  • Charnley, A.K. and Collins, S.A. (2007). Entomopathogenic fungi and their role in pest control. In: Kubicek, C.P. and I.S. Druzhinina (eds.), Environment and microbial relationships. 2nd Ed., The Mycota iv. Springer-Verlag Berlin Heidelberg. pp.159-185.

  • Kumar, D., Roy, C.S., Khan, Z.R., Xazdani, S.S., Hameed, S.F. and Mohamood, M. (1983). An entomogenous fungus Isaria in parasiting mango hoppers. Idioscopes clypealis Sci. & Culture, 49 : 253-254.

  • Lacey, L.A., Frutos, R., Kaya, H.K. and Vails, P. (2001). Insect pathogens as biological control agents: Do they have future? Biol. Contr., 21(3): 230- 248.

  • Lingappa, S., Saxena, H. and Devi Vimala, P.S. (2005). Role of biocontrol agents in management of Helicoverpa armigera (Hubner). In: Recent advances in Helicoverpa armigera Management (Hem Saxena, A. B. Rai, R. Ahmad and Sanjeev Gupta Eds.). Indian Society of Pulses Research and development, IIPR, Kanpur. 159-184 pp.

  • McCoy, C.W., Samson, R.A. and Boucias, D.G. (1988). Entomogenous fungi, p.151236. In : C.M. Ignoffo & N.B. Mandava (eds.), Handbook of natural pesticides, V: Microbial insecticides, Part A: Entomogenous protozoa and fungi. Boca Raton, Fl, CRC Press, 260p.

  • Miller, D.R., Miller, G.L., Hodges, G.S. and Davidson, J.A. (2005). Introduced scale insects (Hemiptera: Coccoidea) of the United States and their impact on U.S. agriculture. Proc. Entomol. Society Washington, 107 (1) : 123–158.

  • Mote, U.N., Mahajan, P.R. and Kadam, J.R. (2003). Bioefficacy of Verticillium lecanii (Zimm.) Viegas against sucking pests of gerbera in polyhouse. In : Green Pesticides for Insect Pest Management, Chennai, pp. 179-183.

  • Nguyen, N.C. Borgemeister, Poehling, H. and Zimmermann, G. (2007). Laboratory investigations on the potential of entomopathogenic fungi for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae and pupae. Biocontrol Sci. Technol., 17(12): 853-864.

  • Nirwan, R.S. and Upadhyaya, J. (1976). Some entomogenous fungi from North India. Indian Phytopathol., 29 : 308-310.

  • Srivastava, R.P. and Tandon, P.L. (1986). Natural occurrence of two entomogenous fungi pathogenic to mango leafhopper. Indian J. Pl. Pathol., 4 : 121-123.

  • Ujian, A.A. and Shahzad, S. (2007). Pathogenicity of Metarhizium anisopliae var. Acridum strains on Pink Hibiscus Mealy bug (Maconellicoccus hirsutus) affecting cotton crop. Pakistan J. Bot., 39 (3): 967-973.

  • Viraktamath, S.A., Vastrad, A.S. and Lingappa, S. (1994). Incidence of the fungus Verticillium lecanii (Zimm.) on mango leafhoppers. Karnataka J. Agric. Sci., 7 : 242-243.

  • Williams, D.J. (1985). Australian mealy bugs. British Museum (Natural History), London, England,431 pp.

  • Williams, D.J. and Granara de Willink, M.C. (1992). Mealy bugs of Central and South America. CAB International, 635 pp., LONDON, ENGLAND.

  • Khuhro, S.N., Kalroo, A.M. and Mahmood, R. (2012).Present status of mealy bug Phenacoccus solenopsis (Tinsley) on cotton and other plants in Sindh (Pakistan). pp. 268–271. In: Book of Papers. World Cotton Research Conference – 5, Mumbai, 07–11 November 2011, 580 pp. https://www.icac. org/meetings/wcrc/ wcrc5/Proceedings.pdf.


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  • In Vitro Efficacy of Beauveria bassiana (Balsamo) Vuill., Metarhizium anisopliae (Metsch.) Sorokin and Lecanicillium lecanii (Zimmerman) against Maconellicoccus hirsutus (Green) and Ferrisia virgata (Cockerell) (Hemiptera:Pseudococcidae)

Abstract Views: 367  |  PDF Views: 1

Authors

Rajesh Illathur
Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
R. Philip Sridhar
Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India

Abstract


Maconellicoccus hirsutus (Green) and Ferrisia virgata (Cockerell) were mass cultured in insectary using hibiscus plants, potato sprouts and the different stages were maintained to conduct bioassay. Entomopathogenic fungi Beauveria bassiana, Metarhizium anispoliae, Lecanicillium lecanii was sub-cultured on SDAY. Spore suspensions of concentration (1x 108conidia/ml) of seven isolates (BbBp1, BbGW1, BbBM1, MaBp1, MaBm1, LlBm1, LlMo1) were prepared from the 15 day old culture of the fungi. A preliminary study on B. bassiana against Maconellicoccus hirsutus (Green) and Ferrisia virgata (Cockerell) female adults was done. untreated (sterile water) were used as controls.Mortality of pink mealy bug (Maconellicoccus hirsutus (Green)) adults under laboratory condition was recorded at different intervals where entomopathogens (% mortality after 9th day) like LlMo1 (93.33%), BbGW1 (80.00%) and MaBm1 (63.33%). The mortality of guava mealy bug [Ferrisia virgata (Cockerell)] adults under laboratory condition was recorded at different intervals where entomopathogens (% mortality after 9th day) like LlMo1 (96.55%), BbBp1 (86.21%) and MaBp1 (65.52%).

Keywords


Beauveria bassiana, Pink Mealy Bug, Guava Mealy Bug, Spore, Mortality.

References