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Utility of Activated Glassy Carbon and Pencil Graphite Electrodes for Voltammetric Determination of Nalbuphine Hydrochloride in Pharmaceutical and Biological Fluids


Affiliations
1 National Organization for Drug Control and Research (NODCAR), P.O. Box 29, Cairo, Egypt
2 Chemistry Department, Faculty of Science, Ain Shams University, Abbasiya 11556, Egypt
3 Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt
 

This work compares voltammetric response of nalbuphine hydrochloride (NP·HCl) at both activated glassy carbon and pencil graphite electrodes. The electrochemical oxidation of the drug was studied using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) techniques. For analytical purpose a well-resolved irreversible diffusion controlled voltammetric peak was established in Britton-Robinson (B-R) buffer solution of pH 6.00 using pencil graphite electrode (PGE). Using activated glassy carbon electrode (GCE) a well-resolved irreversible diffusion controlled voltammetric peak was obtained at pH 7.00 using the same buffer solution. According to the linear relationship between the peak current and NP·HCl concentration, DPV and SWV methods were developed for their quantitative determination in pharmaceutical and human biological fluids.Thelinear response was obtained in the range from 1.6×10-5 to 1.5×10-4 mol L-1 using PGE and from 12.5×10-6 to 13.75 × 10-5mol L-1 using a GC electrode, respectively. Precision and accuracy of the developed method were checked by recovery studies.
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  • Utility of Activated Glassy Carbon and Pencil Graphite Electrodes for Voltammetric Determination of Nalbuphine Hydrochloride in Pharmaceutical and Biological Fluids

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Authors

Hoda M. Elqudaby
National Organization for Drug Control and Research (NODCAR), P.O. Box 29, Cairo, Egypt
Hassan A. M. Hendawy
National Organization for Drug Control and Research (NODCAR), P.O. Box 29, Cairo, Egypt
Eglal R. Souaya
Chemistry Department, Faculty of Science, Ain Shams University, Abbasiya 11556, Egypt
Gehad G. Mohamed
Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt
Ghada M. G. Eldin
National Organization for Drug Control and Research (NODCAR), P.O. Box 29, Cairo, Egypt

Abstract


This work compares voltammetric response of nalbuphine hydrochloride (NP·HCl) at both activated glassy carbon and pencil graphite electrodes. The electrochemical oxidation of the drug was studied using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) techniques. For analytical purpose a well-resolved irreversible diffusion controlled voltammetric peak was established in Britton-Robinson (B-R) buffer solution of pH 6.00 using pencil graphite electrode (PGE). Using activated glassy carbon electrode (GCE) a well-resolved irreversible diffusion controlled voltammetric peak was obtained at pH 7.00 using the same buffer solution. According to the linear relationship between the peak current and NP·HCl concentration, DPV and SWV methods were developed for their quantitative determination in pharmaceutical and human biological fluids.Thelinear response was obtained in the range from 1.6×10-5 to 1.5×10-4 mol L-1 using PGE and from 12.5×10-6 to 13.75 × 10-5mol L-1 using a GC electrode, respectively. Precision and accuracy of the developed method were checked by recovery studies.