International Journal of Kinesiology and Sports Science

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The Effect of Progressive Aerobic Exercise on G6PD Activity among Active and Sedentary Men


Affiliations
1 Department of Sport Physiology, University of Mohaghegh Ardebili Ardebil, Iran, Islamic Republic of
2 Departments of Mathematics and Statistics, University of Mohaghegh Ardebili Ardebil, Iran, Islamic Republic of
3 Department of Sport Physiology, General Department of Fars Province Education Fars, Iran, Islamic Republic of
 

Background: Erythrocyte glucose-6-phosphate dehydrogenase (G6PD) activity is highly associated with free radical production. G6PD deficiency can increase the sensitivity of erythrocytes to oxidative stress resulting in hemolytic anemia. Aim: to study the main effect of progressive aerobic exercise on G6PD activity in active and sedentary men. Material and Methods: the study comprised 10 active men and 10 sedentary men. The protocol, started with running at approximately %75 of their maximal oxygen uptake for 30 min × times a week for y weeks. Venous blood samples (5ml) were collected prior to, immediately after, 2 hours and 24 hours after exercise. G6PD activity was evaluated with auto-Analyzer Method. Result: G6PD was not significantly higher in the active men in comparison with the sedentary men at baseline (10.5 ± 1.2 (IU/gHb) VS 9.5 ± 1.0 (IU/gHb), P ≤ 0.05). G6PD activity was increased significantly in both groups immediately after exercise but was not considerably different between the groups (11.6 ± 2.7 (IU/gHb) VS 9.9 ± 1.1 (IU/gHb), for active and sedentary men, respectively; P ≤ 0.05). G6PD returned to the baseline levels 2 hours after exercise in active men but remained high in sedentary men (10.5 ± 1.4 (IU/gHb) VS 10.1 ± 1.1 (IU/gHb, P ≤ 0.05). Also, G6PD levels showed a significant increase 24 hours after exercise in the active men in comparison with the sedentary men (11.8 ± 2.5 (IU/gHb) VS 9.5 ± 1.5 (IU/gHb), P ≤ 0.05). Conclusion: In this regard, it can be concluded that, progressive aerobic exercise may be an effective factor affecting the levels of G6PD significantly, and as a home message it is useful for controlling the hemolytic anemia among sedentary population.

Keywords

G6PD Activity, Progressive Aerobic Exercise, Hemolytic Anemia.
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  • Ainoon, O, Yu, YH, Amir Muhriz, AL, Boo, NY, Cheong, SK, & Hamidah, NH. (2003). Glucose‐6‐phosphate dehydrogenase (G6PD) variants in Malaysian Malays. Human mutation, 21(1), 101-101.

  • Allahverdiyev, Adil M, Bagirova, Malahat, Elcicek, Serhat, Koc, Rabia Cakir, Ates, Sezen Canim, Baydar, Serap Yesilkir, . . . Oztel, Olga Nehir. (2012). Glucose-6-phosphate dehydrogenase deficiency and malaria: a method to detect primaquine-induced hemolysis in vitro. Dehydrogenases, Prof. Rosa Angela Canuto (Ed.).

  • Banerjee, Alok K, Mandal, Amritlal, Chanda, Dipanjan, & Chakraborti, Sajal. (2003). Oxidant, antioxidant and physical exercise. Molecular and cellular biochemistry, 253(1-2), 307-312.

  • Berzosa, C, Cebrian, I, Fuentes-Broto, L, Gomez-Trullen, E, Piedrafita, E, Martinez-Ballarin, E, . . . Garcia, JJ. (2011). Acute exercise increases plasma total antioxidant status and antioxidant enzyme activities in untrained men. BioMed Research International, 2011.

  • Bloomer, Richard J, Goldfarb, Allan H, Wideman, Laurie, Mckenzie, Michael J, & Consitt, Leslie A. (2005). Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. The Journal of Strength & Conditioning Research, 19(2), 276-285.

  • Bommareddy, Rajesh Reddy, Chen, Zhen, Rappert, Sugima, & Zeng, An-Ping. (2014). A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase. Metabolic Engineering, 25(0), 30-37. doi: http://dx.doi.org/10.1016/j.ymben.2014.06.005

  • Brady, Paul S, Brady, Linda J, & Ullrey, Duane E. (1979). Selenium, vitamin E and the response to swimming stress in the rat. The Journal of nutrition, 109(6), 1103-1109.

  • Fisher-Wellman, Kelsey, & Bloomer, Richard J. (2009). Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine, 8(1), 1.

  • Fisher, Gordon. (2010). Oxidative Stress and Antioxidant Defenses in Lymphocytes Following High Intensity Interval Training. Auburn University.

  • Georgakouli, Kalliopi, Deli, Chariklia K, Zalavras, Athanasios, Fatouros, Ioannis G, Kouretas, Dimitrios, Koutedakis, Yiannis, & Jamurtas, Athanasios Z. (2013). α-Lipoic acid supplementation up-regulates antioxidant capacity in adults with G6PD deficiency. Food and Chemical Toxicology, 61, 69-73.

  • Gomes, Elisa Couto, Silva, Albena Nunes, & Oliveira, Marta Rubino de. (2012). Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species. Oxidative medicine and cellular longevity, 2012.

  • Gonchar, O, & Mankovska, I. (2010). Antioxidant system in adaptation to intermittent hypoxia. Journal of Biological Sciences, 10(6), 545-554.

  • Groussard, C, Rannou-Bekono, F, Machefer, G, Chevanne, M, Vincent, S, Sergent, O, . . . Gratas-Delamarche, A. (2003). Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise. European journal of applied physiology, 89(1), 14-20.

  • Huang, Yang-Yang, Huang, Ching-Shui, Yang, Sien-Sing, Lin, Min-Shung, Huang, May-Jen, & Huang, Ching-Shan. (2005). Effects of variant UDP-glucuronosyltransferase 1A1 gene, glucose-6-phosphate dehydrogenase deficiency and thalassemia on cholelithiasis. World Journal of Gastroenterology, 11(36), 5710.

  • James, S Jill, Cutler, Paul, Melnyk, Stepan, Jernigan, Stefanie, Janak, Laurette, Gaylor, David W, & Neubrander, James A. (2004). Metabolic biomarkers of increased oxidative stress and impaired methylation capacity in children with autism. The American journal of clinical nutrition, 80(6), 1611-1617.

  • Jamurtas, Athanasios Z, Fatouros, Ioannis G, Koukosias, Nikos, Manthou, Eirini, Tofas, Trifon, Yfanti, Christina, . . . Koutedakis, Yiannis. (2006). Effect of exercise on oxidative stress in individuals with glucose-6-phosphate dehydrogenase deficiency. in vivo, 20(6B), 875-880.

  • Ji, Li L, Gomez-Cabrera, Maria-Carmen, & Vina, Jose. (2009). Role of free radicals and antioxidant signaling in skeletal muscle health and pathology. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders), 9(4), 428-444.

  • Machefer, Guillaume, Groussard, Carole, Rannou-Bekono, Francoise, Zouhal, Hassane, Faure, Henry, Vincent, Sophie, . . . Gratas-Delamarche, Arlette. (2004). Extreme running competition decreases blood antioxidant defense capacity. Journal of the American College of Nutrition, 23(4), 358-364.

  • Margaritis, I, Rousseau, AS, Marini, JF, & Chopard, A. (2009). Does antioxidant system adaptive response alleviate related oxidative damage with long term bed rest? Clinical biochemistry, 42(4), 371-379.

  • Masella, Roberta, Di Benedetto, Roberta, Vari, Rosaria, Filesi, Carmela, & Giovannini, Claudio. (2005). Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathionerelated enzymes. The Journal of nutritional biochemistry, 16(10), 577-586.

  • Nazıroglu, Mustafa. (2009). Role of selenium on calcium signaling and oxidative stress-induced molecular pathways in epilepsy. Neurochemical research, 34(12), 2181-2191.

  • Niess, Andreas Michael, & Simon, Perikles. (2007). Response and adaptation of skeletal muscle to exercise-the role of reactive oxygen species. Front Biosci, 12, 4826-4838.

  • Nikolaidis, Michalis G, Jamurtas, Athanasios Z, Paschalis, Vassilis, Kostaropoulos, Iason A, Kladi-Skandali, Athina, Balamitsi, Vera, . . . Kouretas, Dimitris. (2006). Exercise-induced oxidative stress in G6PD-deficient individuals. Medicine and science in sports and exercise, 38(8), 1443-1450.

  • Ohno, H, Yahata, T, Sato, Y, Yamamura, K, & Taniguchi, N. (1988). Physical training and fasting erythrocyte activities of free radical scavenging enzyme systems in sedentary men. European journal of applied physiology and occupational physiology, 57(2), 173-176.

  • Oztasan, Nuray, Taysi, Seyithan, Gumustekin, Kenan, Altinkaynak, Konca, Aktas, Omer, Timur, Handan, . . . Akcay, Fatih. (2004). Endurance training attenuates exercise-induced oxidative stress in erythrocytes in rat. European journal of applied physiology, 91(5-6), 622-627.

  • Petrat, Frank, Pindiur, Stanislaw, Kirsch, Michael, & de Gischolar_main, Herbert. (2003). NAD (P) H, a primary target of 1O2 in mitochondria of intact cells. Journal of Biological Chemistry, 278(5), 3298-3307.

  • Powers, Scott K, & Jackson, Malcolm J. (2008). Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiological reviews, 88(4), 1243-1276.

  • Radak, Zsolt, Chung, Hae Young, & Goto, Sataro. (2008). Systemic adaptation to oxidative challenge induced by regular exercise. Free Radical Biology and Medicine, 44(2), 153-159.

  • Sahlin, Kent, Shabalina, Irina G, Mattsson, C Mikael, Bakkman, Linda, Fernstrom, Maria, Rozhdestvenskaya, Zinaida, . . . Tonkonogi, Michail. (2010). Ultraendurance exercise increases the production of reactive oxygen species in isolated mitochondria from human skeletal muscle. Journal of applied physiology, 108(4), 780-787.

  • Schafer, Freya Q, & Buettner, Garry R. (2001). Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radical Biology and Medicine, 30(11), 1191-1212.

  • Schulpis, Kleopatra H, Reclos, George J, Parthimos, Theodore, Parthimos, Nickolaos, Gavriilidis, Andreas, & Tsakiris, Stylianos. (2006). L-cysteine supplementation protects the erythrocyte glucose-6-phosphate dehydrogenase activity from reduction induced by forced training. Clinical biochemistry, 39(10), 1002-1006.

  • Sen, Chandan K. (1995). Oxidants and antioxidants in exercise. Journal of Applied Physiology, 79(3), 675-686.

  • Shariat, Ardalan, Kargarfard, Mehdi, Tamrin, Shamsul Bahri Mohd, Danaee, Mahmoud, & Karimi, Hossein. (2014). Strength-training and biological rhythm of male sex hormone among judoists. Biological Rhythm Research(ahead-ofprint), 1-7.

  • Spodaryk, Krzysztof. (2001). The influence of low-power laser energy on red blood cell metabolism and deformability. Clinical hemorheology and microcirculation, 25(3), 145-151.

  • Spodaryk, Krzysztof, Szyguła, Zbigniew, Dabrowski, Zbigniew, & Miszta, Helena. (1985). The activity of erythrocyte enzymes in rats subjected to running exercises. European journal of applied physiology and occupational physiology, 54(5), 533-537.

  • Theodorou, Anastasios A, Nikolaidis, Michalis G, Paschalis, Vassilis, Sakellariou, Georgios K, Fatouros, Ioannis G, Koutedakis, Yiannis, & Jamurtas, Athanasios Z. (2010). Comparison between glucose-6-phosphate dehydrogenasedeficient and normal individuals after eccentric exercise. Med Sci Sports Exerc, 42(6), 1113-1121.

  • Tsakiris, Stylianos, Parthimos, Theodore, Parthimos, Nickolaos, Tsakiris, Theodore, & Schulpis, Kleopatra H. (2006). The beneficial effect of L-cysteine supplementation on DNA oxidation induced by forced training. Pharmacological research, 53(4), 386-390.

  • Tsakiris, Stylianos, Reclos, George J, Parthimos, Theodore, Tsakiris, Theodore, Parthimos, Nickolaos, & Schulpis, Kleopatra H. (2006). α-Tocopherol supplementation restores the reduction of erythrocyte glucose-6-phosphate dehydrogenase activity induced by forced training. Pharmacological research, 54(5), 373-379.

  • Tsakiris, Stylianos, Schulpis, Kleopatra H, Marinou, Kyriakoula, & Behrakis, Panagiotis. (2004). Protective effect of Lcysteine and glutathione on the modulated suckling rat brain Na+, K+,-ATPase and Mg2+-ATPase activities induced by the in vitro galactosaemia. Pharmacological research: the official journal of the Italian Pharmacological Society, 49(5), 475-479.

  • Urso, Maria L, & Clarkson, Priscilla M. (2003). Oxidative stress, exercise, and antioxidant supplementation. Toxicology, 189(1), 41-54.

  • Vesovic, D, Borjanovic, S, Markovic, S, & Vidakovic, A. (2001). Strenuous exercise and action of antioxidant enzymes. La Medicina del lavoro, 93(6), 540-550.

  • Vina, Jose, Borras, Consuelo, Gomez-Cabrera, Mari-Carmen, & Orr, William C. (2006). Part of the Series: From Dietary Antioxidants to Regulators in Cellular Signalling and Gene ExpressionRole of reactive oxygen species and (phyto) oestrogens in the modulation of adaptive response to stress: Review. Free radical research, 40(2), 111-119.

  • Wamelink, MMC, Struys, EA, & Jakobs, C. (2008). The biochemistry, metabolism and inherited defects of the pentose phosphate pathway: a review. Journal of inherited metabolic disease, 31(6), 703-717.


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  • The Effect of Progressive Aerobic Exercise on G6PD Activity among Active and Sedentary Men

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Authors

Amin Allah Dashtiyan
Department of Sport Physiology, University of Mohaghegh Ardebili Ardebil, Iran, Islamic Republic of
Marefat Siahkouhian
Department of Sport Physiology, University of Mohaghegh Ardebili Ardebil, Iran, Islamic Republic of
Masoud Ganj
Departments of Mathematics and Statistics, University of Mohaghegh Ardebili Ardebil, Iran, Islamic Republic of
Aidin Valizadeh Oranj
Department of Sport Physiology, University of Mohaghegh Ardebili Ardebil, Iran, Islamic Republic of
Hadi Bashafaat
Department of Sport Physiology, General Department of Fars Province Education Fars, Iran, Islamic Republic of

Abstract


Background: Erythrocyte glucose-6-phosphate dehydrogenase (G6PD) activity is highly associated with free radical production. G6PD deficiency can increase the sensitivity of erythrocytes to oxidative stress resulting in hemolytic anemia. Aim: to study the main effect of progressive aerobic exercise on G6PD activity in active and sedentary men. Material and Methods: the study comprised 10 active men and 10 sedentary men. The protocol, started with running at approximately %75 of their maximal oxygen uptake for 30 min × times a week for y weeks. Venous blood samples (5ml) were collected prior to, immediately after, 2 hours and 24 hours after exercise. G6PD activity was evaluated with auto-Analyzer Method. Result: G6PD was not significantly higher in the active men in comparison with the sedentary men at baseline (10.5 ± 1.2 (IU/gHb) VS 9.5 ± 1.0 (IU/gHb), P ≤ 0.05). G6PD activity was increased significantly in both groups immediately after exercise but was not considerably different between the groups (11.6 ± 2.7 (IU/gHb) VS 9.9 ± 1.1 (IU/gHb), for active and sedentary men, respectively; P ≤ 0.05). G6PD returned to the baseline levels 2 hours after exercise in active men but remained high in sedentary men (10.5 ± 1.4 (IU/gHb) VS 10.1 ± 1.1 (IU/gHb, P ≤ 0.05). Also, G6PD levels showed a significant increase 24 hours after exercise in the active men in comparison with the sedentary men (11.8 ± 2.5 (IU/gHb) VS 9.5 ± 1.5 (IU/gHb), P ≤ 0.05). Conclusion: In this regard, it can be concluded that, progressive aerobic exercise may be an effective factor affecting the levels of G6PD significantly, and as a home message it is useful for controlling the hemolytic anemia among sedentary population.

Keywords


G6PD Activity, Progressive Aerobic Exercise, Hemolytic Anemia.

References