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Prevalence and Antibiogram of Uropathogens in a Tertiary Care Hospital in Manipur, India
Introduction: Due to high variability of resistance patterns which vary even over short periods of time, periodic evaluation of such activity is essential. Aim: To document the prevalence, antibiotic susceptibility and resistance patterns of uropathogens in the area to ensure appropriate therapy. Methods: This is a cross sectional study on 5108 urine samples from June 2014 to May 2015. Antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion method and compared. Gram positive cocci and Gram negative bacilli detected were further subjected to detection of Methicillin resistant Staphyloccocussureus (MRSA) by Cefoxitin disc diffusion tests and detection of Extended Spectrum Beta lactamases (ESBL) by ESBL screening test and confirmed by combined disc diffusion test(CDT). Results: Out of 5108 samples subjected to bacterial culture, 2940(57.56%) showed positive growth out of which Escherichia coli, 1300(44.2%) was the most common organism isolated followed by Staphylococcus aureus 914(31%), Klebsiella pneumonia 362(12.3%), Enterococcus 172(5.85%), Acinetobacter 80(2.72%), Pseudomonas 72(2.44%), Proteus 40(0.81%), and Staphylococcus saprophyticus 16(0.54%). On further evaluation, 1026(56.06%) isolates of Gram negative bacilli(GNB) family showed presence of ESBL and 722(78.99%) isolates of Staphylococcus aureus were positive for (MRSA) tests. Conclusion: Continual surveillance is required to detect changes in prevalence rates of different uropathogens. Increased prevalence of Acinetobacter was detected in our study. Monitoring of MRSA, ESBL production and antimicrobial susceptibility testing (AST) is necessary to avoid treatment failure and development of further resistance in patients with UTI.
Urinary Tract Infection, Acinetobacter.
- Stamm WE, Norrby SR. Urinary tract infections: disease panorama and challenges. J Infect Dis 2001;183: S1-S4.
- Daoud Z, Sokhn ES, Mastri K, Cheaito K, Haidar-Ahmad N, Matar GM, et al. Escherichia coli isolated from urinary tract infections of lebanese patients between 2005 and 2012: Epidemiology and Profiles of Resistance. Front Med (Lausanne) 2015;2(66).
- Shaaban MT, Ghozlan HA, El Maghraby MM. Susceptibility of bacteria infecting urinary tract to some antibiotics and essential oils. Journal of Applied Pharmaceutical Science 2012;02(04):90-98.
- Gupta V, Yadav A, Joshi R M. Antibiotic resistance pattern in uropathogens. Indian J Med Microbiol 2002;20;96-8.
- Colle JG, Dugoid JP, Fraser AG. Laboratory strategy in the diagnosis of infective syndrome. In: Colle JG, Dugoid JP, Fraser AG, Editors. Mackie and Mc-Cartney Practical Medical Microbiology 14thed. London:Churchill Livingstone; 1996.p.53-94.
- Hanna-Wakim RH, Ghanem ST, El Helou MW, Khafaja SA, Shaker RA, Hassan SA, et al. Epidemiology and characteristics of urinary tract infections in children and adolescents. Front Cell Infect Microbiol 2015;5:45.
- Koneman EW, Allen SD, Janda WM, Schreckenberger PC. The Enterobacteriaceae. In: Koneman’s color atlas and textbook of diagnostic microbiology. 6thed. Philadelphia PA: Lippincott Williams and Wilkins; 2006.p. 53-94.
- Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J ClinPathol 1966;45:493-6.
- Clinical Laboratory Standards Institute. Performance Standard for Antimicrobial Susceptibility Testing. NCCLS Document 2013;23 Suppl:M100-S23.
- Jain A, Agarwal A,Verma RK. Cefoxitin disc diffusion test for detection of methicillin-resistant staphylococci. Journal of Medical Microbiology 2008;57;957-61.
- Singh RM, Singh HL. Comparative evaluation of six phenotypic methods for detecting extended-spectrum beta-lactamase-producing enterobacteriaceae. J Infect Dev Ctries 2014;8(04):408-15.
- Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MA. Antibiotic resistance and extended-spectrum beta-lactamases: types, epidemiology and treatment. Saudi J Biol Sci 2015;22:90–101.
- Deshpande KD, Pichare AP, Suryawanshi NM, Davane MS. Antibiogram of gram negative uropathogens in hospitalized patients. Int J Recent Trends SciTechnol 2011;1(2):56–60.
- Urvashi C, Chitra Y, Lokhendro H. Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern of patients attending jnims hospital, Imphal. J Evolution Med Dent Sci 2013;2(50):9769-74.
- Benachinmardi K, Padmavathy M, Malini J, Navaneet BV. Microbiological profile and antibiogram of uropathogens in paediatric age group. Int J of Health Allied Sci 2015;4(1):61-4.
- Madhavi VN, Subbulu P. Prevalence and antibacterial susceptibility testing pattern of bacterial pathogens causing urinary tract infections in community. J Evolution Med Dent Sci 2016;5(30):1528-31.
- Haque R, Akter ML, Salam MA. Prevalence and susceptibility of uropathogens: a recent report from a teaching hospital in bangladesh. BMC Res Notes 2015;8:416.
- Kothari A, Sagar V. Antibiotic resistance in pathogens causing community-acquired urinary tract infections in India: a multicenter study. J Infect Dev Ctries 2008;2:354–8.
- Gade ND, Qazi MS. Fluoroquinolone therapy in staphylococcus aureus infections: where do we stand? J Lab Phys 2013;5:109–12.
- Gales AC, Jones RN, Forward KR, Inares JL, Sader HS, Verhoef J. Emerging importance of multidrug-resistant acinetobacter species and stenotrophomonasmaltophilia as pathogens in seriously ill patients: geographic patterns, epidemiological features, and trends in the sentry antimicrobial surveillance program (1997-1999). Clinical Infectious Diseases 2001;32(Suppl 2);104-13.
- Rekha B, Pai R, Scaria B, Shetty A, Pinto H. Microbiological profile of the uropathogens isolated from pediatric patients from a tertiary care centre. Karnataka Paediatr J 2010;24:42-5.
- Saha A, Majumdar T, Dasgupta A, Saumandal P. Prevalence of extended spectrum beta-lactamases (ESBLs) among uropathogens at a tertiary care hospital in Tripura. The health Agenda 2015;3(2):55-62.
- Taneja N, Rao P, Arora J, Dogra A. Occurrence of ESBL and Amp-C beta-lactamases and susceptibility to newer antimicrobial agents in complicated UTI. Indian J Med Res 2008;127:85-8.
- Joshi S, Ray P, Manchanda V, Bajaj J, Chitnis DS, Gautam V. et al. Methicillin resistant staphylococcus aureus (MRSA) in India: prevalence & susceptibility pattern. Indian J Med Res 2013;137(2);363-69.
- Broekema NM, Van Tam T, Monson TA, Marshall SA, Warshauer DM. Comparison of cefoxitin and oxacillin disk diffusion methods for detection of meca-mediated resistance in staphylococcus aureus in a large-scale study. Journal of Clinical Microbiology 2009;47(1):217–9.
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