Journal of Horticultural Sciences

Reena Rosy Thomas ¹, M. K. Chandra Prakash ¹, M. Krishna Reddy ², Sukhada Mohandas ³, Riaz Mahmood ⁴

Affiliations
1 Section of Economics and Statistics, Indian Institute of Horticultural Research, Hessaraghatta, Bangalore -560089, IN
2 Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore - 560 089, IN
3 Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore - 560 089, IN
4 Dept. of Biotechnology, Kuvempu University, Shimoga - 577 451, IN

Abstract

Abiotic stress often causes a series of morphological, physiological, biochemical and molecular changes that affect plant growth, development and productivity. To cope with abiotic stresses, it is necessary to understand plant responses to stresses that disturb homeostatic equilibrium at the cellular and molecular level. Genomic information on Capsicum annuum has been explored to identify microsatellite markers associated with abiotic stress tolerance and assign them to cognate functional groups related to specific stress responses. Several in silico methods have been used to identify simple sequence repeats associated with stress responsive gene candidates in Capsicum annuum. In this study, a microsatellite marker has been identified in Capsicum annuum associated with Nucleoside Diphosphate Kinase (NDK) having multiple environmental stress tolerance (oxidative, high temperature and salt stress) and which is also highly conserved in crops of Solanaceae. These are house-keeping enzymes that maintain intracellular levels of all nucleoside triphosphates (NTP) with the exception of adenosine triphosphate (ATP). These are also involved in phytochrome A response, UV-B signaling, auxin responses and oxidative stress signaling.

Keywords

Nucleoside Diphosphate Kinase (NDK), Microsatellite, Abiotic Stress, Solanaceae.

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Vageeshbabu S. Hanur ¹, D. P. Prakash ¹, B. S. Deepali ¹, R. Asokan ¹, Y. L. Ramachandra ², Riaz Mahmood ², Lalitha Anand ¹

Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN

Abstract

Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.

Keywords

Solanum melongena, Transformation, Hypocotyl, BAP, Preconditioning.

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