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Asokan, R.
- Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
Abstract Views :208 |
PDF Views:108
Authors
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Lalitha Anand
1,
Vageeshbabu S. Hanur
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 2, No 2 (2007), Pagination: 94-98Abstract
Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.Keywords
Growth Regulators, Explant, Brinjal, Transformation.- Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
Abstract Views :225 |
PDF Views:112
Authors
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Lalitha Anand
1,
Vageeshbabu S. Hanur
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 2, No 1 (2007), Pagination: 19-25Abstract
A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.Keywords
Solanum melongena, Kanamycin, Cefotaxime, Gelling Agents, Transformation.- Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
Abstract Views :214 |
PDF Views:112
Authors
Vageeshbabu S. Hanur
1,
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Riaz Mahmood
2,
Lalitha Anand
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 1, No 2 (2006), Pagination: 116-119Abstract
Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.Keywords
Solanum melongena, Transformation, Hypocotyl, BAP, Preconditioning.- Comparative Toxicity of Two Isolates of Bacillus thuringiensis Berliner from Plutella xylostella L. and Papilio demoleus L. to some Important Lepidopteran Pests of Horticultural Crops
Abstract Views :175 |
PDF Views:94
Authors
R. Asokan
1,
Puttaswamy
2
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake (PO), Bangalore – 560 089, IN
2 Department of Entomology, University of Agricultural Sciences, G. K. V. K., Bangalore – 560 065, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake (PO), Bangalore – 560 089, IN
2 Department of Entomology, University of Agricultural Sciences, G. K. V. K., Bangalore – 560 065, IN