Journal of Horticultural Sciences

C. Kavitha ¹, E. Vadivel ², R. Sivasamy ³, K. Rajamani ¹

Affiliations
1 Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, IN
2 TNAU, Coimbatore-641003, IN
3 Department of Biotechnology, Bharathiyar University, Coimbatore-641046, IN

Abstract

Coleus forskohlii Briq. is an indigenous medicinal plant with high traditional use in India. Genetic analysis of 37 diverse C. forskohlii genotypes was performed using 25 RAPD primers, which yielded 117 bands, of which 60 (51.28%) were polymorphic providing an average of 3.75 bands per primer. There were no genotype-specific products. The number of bands per primer varied from 1 (OPZ 8&16) to 7 (OPZ 11). Similarity matrix was constructed using Jaccard's Coefficient and the data matrix of coefficient of similarity was subjected to cluster analysis using unweighted pair group methodology with arithmetic average (UPGMA). Cluster analysis resulted in grouping of 37 genotypes into two major clusters. The results indicated that RAPD could be used for genetic diversity analysis in C. forskohlii using higher number of primers as it is reliable, easy, rapid and cost-effective.

Keywords

Coleus forskohlii, Genetic Diversity, RAPD.

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C. Kavitha ¹, E. Vadivel ², K. Rajamani ¹, C. Thangamani ¹

Affiliations
1 Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore-641 003, IN
2 Directorate of Extension Education, TNAU, Coimbatore – 641 003, IN

Abstract

Thirty seven Coleus forskohlii genotypes collected from different regions of Tamil Nadu and Karnataka were subjected to diversity analysis based on NBPGR descriptors. Eleven qualitative and fourteen quantitative traits of C. forskohlii were evaluated to assess the morphological variations available among the collected genotypes. For qualitative traits, a large number of genotypes out of 37 clustered together at 74 % similarity in four different groups. The dendrogram contract based on fourteen quantitative traits for the same set of genotypes did not reveal a clear pattern in grouping and the genotypes were grouped into ten different clusters. Cluster analysis of various sets of data revealed different groups of genotypes for each of the data set. A poor congruence observed among data sets of qualitative and quantitative traits in the comparison indicated that the morphological traits are not suitable for precise discrimination of closely related genotypes in C. forskohlii.

Keywords

Coleus forskohlii, Morphological Traits, Cluster Analysis.

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R. Chitra ¹, E. Vadivel ¹, K. Rajamani ¹

Affiliations
1 Horticulture College and Research Institute, Tamil Nadu Agricultural University, Coimbatore 641 003, IN

Abstract

Phyllanthus amarus Schum. and Thonn (Euphorbiaceae) is recognized commonly as 'Bhumyamlaki' in the Indian system of medicine and has been traditionally used for treating a variety of ailments, including hepatic disorders. Anti-hepatic viral compounds such as phyllanthin and hypophyllanthin were evaluated in different types of in vitro cultures of Phyllanthus amarus by High Performance Liquid Chromatography (HPLC). Among the cultures, in vitro plantlets regenerating from the nodal segment recorded higher amounts of phyllanthin and hypophyllanthin.

Keywords

Phyllanthus amarus, HPLC, Phyllanthin and Hypophyllanthin.

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