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Assessment of Genetic Divergence in Long Day Onion (Allium cepa L.) through Principal Component and Single Linkage Cluster Analysis


Affiliations
1 ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, India
 

To assess the nature and magnitude of genetic diversity in long day onion germplasm by using the principal component analysis and single linkage cluster analysis an experiment was carried out with 34 onion genotypes. High coefficient of variation with wide range in traits indicated an appreciable variability in germplasm. Genotypes were classified into seven principal components having Eigen value > 1, cumulatively accounted for 83.87% of total variability. Principal Component - I contributed for 24.73% of total variation for followed by principal component-II (15.27%). PC-I had high positive loading for bulb weight (0.401), marketable yield (0.338), total bulb yield (0.401) and PC-II had high positive loading for plant height (0.412), PC-III for high T.S.S. (0.276) PC -IV for A grade bulbs (0.436), PC-V for polar diameter of bulbs (0.514), PC-IV negatively loaded with purple blotch (-0.461) and PC-VII for narrow neck thickness (-0.515). Plotting PC-I aganist PC-II differenciated CITH-O-13, CITH-O-4, CITH-O-22, CITH-O-19, CITH-O-9, CITH-O-6 and CITH-O2 as most divergent genotype.On the basis of single linkage cluster means cluster-I was most importent for average bulb weight, minimum bolters, high marketble bulb percentage high marketable and total bulb yield whereas cluster -II was important for maximum nuber of leaves/plant and minimum neck thicknes. Highest inter-cluster distance was observed between cluter II and Cluster-I(873.5%).Most divergent genotypes with high inter cluter distance could be the most appropriate parents for crop impovement in onion.

Keywords

Genetic Diversity, Onion, Principal Component Analysis, Single Linkage Cluster Analysis.
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  • Ario, O. J. and Odulaza, A. 1991. Numerical analysis of variation among accessions of okra. (A. esculentus L. Moench) Malvaceae. Ann. Bot. 67:527-531
  • Arya, J. S., Singh, N. Arya, P. and Kant A.2017. Morphological variation and relationship among the onion germplasm for quantitative and qualitative traits at trans Himalayas Ladakh India. Australian J. Crop Sci.11 (3)229-37.
  • Chattopadhay, A. Sarangi, A.B., Dutta, S., Das,S. and Deney, M. 2015. Genetic relatedness between qualitative and qualitative parameters in onion (Allium cepa L). Vegetose. An International Journal of Plant Research. 26 (1):151-157.
  • Denton, O. A. and Nwangburuka, C. C. 2011.Genetic Variability in eighteen cultivars of wolanum anguiviam L. using principal component analysis (PCA) and single linkage lluster Analysis (SLCA). Ann. Biol. Res. 2 : 62-67
  • Kandil, A.A., Leila, A.A., Mostafa A.K., Fathalla, F.H. (2010) Study on internal bulb quality of some new Egyptian onion cultivars under different irrigation regime. International J. Plant Production. 1(2) : 205-212
  • Kendall, M.A.1980.Multivariate Analysis (2ndEdn.) Charls Griffin and Co. Lonon.
  • Meena, O.P. and Bahadur, V. 2015. Breeding potential of indeterminate tomato (Solanum lycopersicum L) accessions using D2 analysis. SARRAO J. Bree. Gen. 47(1) 49-59.
  • Peter, J. P. and Martinelli, J.A.1989. Hierarchical cluster analysis as a tool to manage the variation in germplasm collection. Theor. Appl. Genet. 78:42-48
  • Ravindra, D., Amirender, K. and Anil, K. 2018.Genetic variability, heritability and diversity analysis in short day tropical onion (Allium cepa L):
  • Santara, P. Manna, D., Sarkar, H. K. and Maithy, T.K. 2017. Genetic variability, heritability genetic advance in kharif onion (Allium cepa L). Journal of Crop and Weed. 13(1):103-106
  • SAS Institute, 2011. SAS Institute Inc., SAS 9.1.3 Help and Documentation, Cary, NC: SAS Institute Inc., 2002-2004.
  • Shalini, M., Sharma., S. Gupta, M.M. and Sushi K. 2003: Evaluation of an Indian germplasm collection of the medicinal plant Bacopa monnieri (L.) Pennell by use of Multivariate approaches. Euphytica 133 : 255-265.
  • Singh, R. K. and Choudhry, B. D. 1985. Biometrical Methods in Quantitative GeneticAnalysis. Kalyani Publishers, New Delhi, p.318.
  • Singh, S.R., Lal S., Ahmed N., Srivastava K.K., Kumar D., Jan N., Amin A., and Malik A.R. 2013. Determination of genetic diversity in onion Allium cepa L) using the multivariate analysis under long day conditions. Afri. J. Biot. 7 (20) 5599-606.
  • Singh, S.R., Ahmed N. , Srivastava K.K., Singh D.B., Achal, Singh, and Yousuf S. 2017. Genetic divergence assessment in European carrot type. Indian J. Hort. 74 (1):551-54
  • Steel, R.G, and Torrie, R.H. 1980. Principles and procedure of statistics. 2nd edition McGraw-Hill, Inc. New York.

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  • Assessment of Genetic Divergence in Long Day Onion (Allium cepa L.) through Principal Component and Single Linkage Cluster Analysis

Abstract Views: 462  |  PDF Views: 176

Authors

S. R. Singh
ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, India
N. Ahamed
ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, India
K. K. Srivastava
ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, India
D. Kumar
ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, India
S. Yousuf
ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, India

Abstract


To assess the nature and magnitude of genetic diversity in long day onion germplasm by using the principal component analysis and single linkage cluster analysis an experiment was carried out with 34 onion genotypes. High coefficient of variation with wide range in traits indicated an appreciable variability in germplasm. Genotypes were classified into seven principal components having Eigen value > 1, cumulatively accounted for 83.87% of total variability. Principal Component - I contributed for 24.73% of total variation for followed by principal component-II (15.27%). PC-I had high positive loading for bulb weight (0.401), marketable yield (0.338), total bulb yield (0.401) and PC-II had high positive loading for plant height (0.412), PC-III for high T.S.S. (0.276) PC -IV for A grade bulbs (0.436), PC-V for polar diameter of bulbs (0.514), PC-IV negatively loaded with purple blotch (-0.461) and PC-VII for narrow neck thickness (-0.515). Plotting PC-I aganist PC-II differenciated CITH-O-13, CITH-O-4, CITH-O-22, CITH-O-19, CITH-O-9, CITH-O-6 and CITH-O2 as most divergent genotype.On the basis of single linkage cluster means cluster-I was most importent for average bulb weight, minimum bolters, high marketble bulb percentage high marketable and total bulb yield whereas cluster -II was important for maximum nuber of leaves/plant and minimum neck thicknes. Highest inter-cluster distance was observed between cluter II and Cluster-I(873.5%).Most divergent genotypes with high inter cluter distance could be the most appropriate parents for crop impovement in onion.

Keywords


Genetic Diversity, Onion, Principal Component Analysis, Single Linkage Cluster Analysis.

References