Journal of Horticultural Sciences

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Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)


Affiliations
1 Department of Floriculture, Medicinal and Aromatic Plants, Uttar Banga Krishi Viswavidyalaya, Cooch Behar, West Bengal, India
 

An experiment was undertaken to develop an improved in vitro regeneration protocol in gerbera. Murashige and Skoog (MS) medium was supplemented with various growth regulators at different concentrations for callus induction and organogenesis. Newly emerging leaves of Gerbera cv. Rosalin were used as explants. Experimental results showed that maximum rate (74.07%) of formation of callus with good growth was recorded on MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1. Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium. Maximum (66.7 %) and earliest (12.3 days) ischolar_main formation in shoots was recorded on IBA 3.0mgL-1 is 1/2MS media. Survival rate of regenerated plantlets was maximum (73.33 %) in the potting mixture containing garden soil, sand and vermicompost (1:1:1).

Keywords

Gerbera, Gerbera jamesonii, Leaf Explants, Callus, in Vitro Regeneration.
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  • Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)

Abstract Views: 337  |  PDF Views: 125

Authors

Koushik Dutta
Department of Floriculture, Medicinal and Aromatic Plants, Uttar Banga Krishi Viswavidyalaya, Cooch Behar, West Bengal, India
Subhendu S. Gantait
Department of Floriculture, Medicinal and Aromatic Plants, Uttar Banga Krishi Viswavidyalaya, Cooch Behar, West Bengal, India

Abstract


An experiment was undertaken to develop an improved in vitro regeneration protocol in gerbera. Murashige and Skoog (MS) medium was supplemented with various growth regulators at different concentrations for callus induction and organogenesis. Newly emerging leaves of Gerbera cv. Rosalin were used as explants. Experimental results showed that maximum rate (74.07%) of formation of callus with good growth was recorded on MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1. Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium. Maximum (66.7 %) and earliest (12.3 days) ischolar_main formation in shoots was recorded on IBA 3.0mgL-1 is 1/2MS media. Survival rate of regenerated plantlets was maximum (73.33 %) in the potting mixture containing garden soil, sand and vermicompost (1:1:1).

Keywords


Gerbera, Gerbera jamesonii, Leaf Explants, Callus, in Vitro Regeneration.

References