Journal of Horticultural Sciences

Open Access Open Access  Restricted Access Subscription Access

Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota


Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, India
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, India
 

A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.

Keywords

Solanum melongena, Kanamycin, Cefotaxime, Gelling Agents, Transformation.
User
Notifications
Font Size

Abstract Views: 224

PDF Views: 112




  • Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota

Abstract Views: 224  |  PDF Views: 112

Authors

D. P. Prakash
Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, India
B. S. Deepali
Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, India
R. Asokan
Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, India
Y. L. Ramachandra
Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, India
Lalitha Anand
Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, India
Vageeshbabu S. Hanur
Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, India

Abstract


A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.

Keywords


Solanum melongena, Kanamycin, Cefotaxime, Gelling Agents, Transformation.