Himachal Journal of Agricultural Research

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Brain and Brain Stem Harvesting Technique in Prenatal Gaddi Sheep Fetuses


Affiliations
1 Department of Veterinary Anatomy, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India
 

A modified approach to harvest fetal brain along with brainstem in small ruminants was designed. A total of 54 number of Gaddi sheep fetuses were used in the present study. With no marked abnormality, the fetuses were aged between 30 days of gestation until full term. The brain and brainstem was harvested by removal of cranial vault via circular incision around it and cruciform incision along the sutures on its dorsal aspect. The squamous part of occipital bones was removed by wedge shaped incision. The resultant sample can be used for autopsy, neurosurgical, developmental and anatomical studies.

Keywords

Brain, Removal, Brainstem, Fetus, Sheep, Craniotomy.
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  • Brain and Brain Stem Harvesting Technique in Prenatal Gaddi Sheep Fetuses

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Authors

Brij Vanita
Department of Veterinary Anatomy, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India
Rajesh Rajput
Department of Veterinary Anatomy, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India
Virender Pathak
Department of Veterinary Anatomy, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India

Abstract


A modified approach to harvest fetal brain along with brainstem in small ruminants was designed. A total of 54 number of Gaddi sheep fetuses were used in the present study. With no marked abnormality, the fetuses were aged between 30 days of gestation until full term. The brain and brainstem was harvested by removal of cranial vault via circular incision around it and cruciform incision along the sutures on its dorsal aspect. The squamous part of occipital bones was removed by wedge shaped incision. The resultant sample can be used for autopsy, neurosurgical, developmental and anatomical studies.

Keywords


Brain, Removal, Brainstem, Fetus, Sheep, Craniotomy.

References