Himachal Journal of Agricultural Research

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Screening of Potential Wheat Genotypes for Drought Tolerance using Polyethylene Glycol


Affiliations
1 Molecular Cytogenetics and Tissue Culture Lab (MCTL), Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
2 Vice-Chancellor, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
 

Screening for early drought tolerance by using three treatments of PEG-6000, control (T1), 15% (T2 ) and 25% (T3) was employed in six wheat genotypes (DH 114, DH 40, DH 65, C 306, DH 52 and DH 5). The genotypes were 3 evaluated for shoot length, coleoptile length, ischolar_main length and ischolar_main to shoot ratio. From the results, shoot, coleoptile and ischolar_main length decreased with increased PEG concentrations. DH 40 expressed highest shoot length in T and T with least reduction and C 306 witnessed longer coleoptile with least reduction over control. DH 114 2 3 expressed highest ischolar_main length in all treatments, while DH 40 had least reduction (3.31 and 7.96% in T2 and T3, respectively). C 306 in T3 and DH 114 in T2 were superior for ischolar_main to shoot ratio. DH 40, DH 114 and C 306 3 2 possessed early drought tolerance behaviour and might be productive in wheat improvement programmes.

Keywords

Coleoptile Length, DH 40, Drought Tolerance, PEG.
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  • Screening of Potential Wheat Genotypes for Drought Tolerance using Polyethylene Glycol

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Authors

N. V. Manoj
Molecular Cytogenetics and Tissue Culture Lab (MCTL), Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
H. K. Chaudhary
Vice-Chancellor, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India
Kritika Singh
Molecular Cytogenetics and Tissue Culture Lab (MCTL), Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, India

Abstract


Screening for early drought tolerance by using three treatments of PEG-6000, control (T1), 15% (T2 ) and 25% (T3) was employed in six wheat genotypes (DH 114, DH 40, DH 65, C 306, DH 52 and DH 5). The genotypes were 3 evaluated for shoot length, coleoptile length, ischolar_main length and ischolar_main to shoot ratio. From the results, shoot, coleoptile and ischolar_main length decreased with increased PEG concentrations. DH 40 expressed highest shoot length in T and T with least reduction and C 306 witnessed longer coleoptile with least reduction over control. DH 114 2 3 expressed highest ischolar_main length in all treatments, while DH 40 had least reduction (3.31 and 7.96% in T2 and T3, respectively). C 306 in T3 and DH 114 in T2 were superior for ischolar_main to shoot ratio. DH 40, DH 114 and C 306 3 2 possessed early drought tolerance behaviour and might be productive in wheat improvement programmes.

Keywords


Coleoptile Length, DH 40, Drought Tolerance, PEG.

References