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Garg, Sandeep
- Phosphate Solubilization Mechanisms in Alkaliphilic Bacterium Bacillus marisflavi FA7
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1 Department of Microbiology, Goa University, Panjim 403 206, IN
2 Department of Microbiology, P.E.S’s Shri Ravi S. Naik College of Arts and Science, Ponda 403 401, IN
1 Department of Microbiology, Goa University, Panjim 403 206, IN
2 Department of Microbiology, P.E.S’s Shri Ravi S. Naik College of Arts and Science, Ponda 403 401, IN
Source
Current Science, Vol 114, No 04 (2018), Pagination: 845-853Abstract
This study reports the mechanisms of phosphate solubilization present in alkaliphilic Bacillus marisflavi FA7. The strain obtained from sediment samples of mangrove ecosystem exhibited different mechanisms to solubilize inorganic phosphate and mineralize organic phosphate under alkaline conditions. It reduced the pH of the medium that showed near perfect correlation with tri-calcium phosphate solubilization. Organic acids produced by the strain were detected in broth. Maximum decrease in pH of the medium was observed with NH4Cl as an inorganic nitrogen source. This indicated involvement of proton extrusion mechanism toward phosphate solubilization during ammonium uptake. It produced exopolysaccharide, but failed to produce siderophore. Bacillus marisflavi FA7 produced extracellular alkaline phosphatase having molecular weight of 175–200 kDa. The pH optimum for maximum enzyme activity was 10.1 and Km of 1.13 μM p-nitrophenolphosphate. This is the first study to report the highest tri-calcium phosphate solubilization by an alkaliphilic bacterium.Keywords
Alkaliphilic Bacterium, Bacillus, Mechanism, Phosphate Solubilization, Phosphatase.Full Text
References
- Illmer, S. and Schinner, F., Solubilization of inorganic calcium phosphates. Soil. Biol. Biochem., 1995, 27, 257–263.
- Zhu, Y., Wu, F., Feng, W., Liu, S. and Giesy, J. P., Interaction of alkaline phosphatase with minerals and sediments: activities, kinetics and hydrolysis of organic phosphorus. Colloids Surf. A Physicochem. Eng. Asp., 2016, 495, 46–53.
- Spohn, M., Treichel, N. S., Cormann, M., Schloter, M. and Fischer, D., Distribution of phosphatase activity and various bacterial phyla in the rhizosphere of Hordeum vulgare L. depending on P availability. Soil. Biol. Biochem., 2015, 89, 44–51.
- Illmer, P. and Schinner, F., Solubilization of inorganic phosphates by microorganisms isolated from forest soils. Soil Biol. Biochem., 1992, 24(4), 389–395.
- Paul, D. and Sinha, N., Isolation and characterization of a phosphate solubilizing heavy metal tolerant bacterium from River Ganga, West Bengal, India. Songklanakarin J. Sci. Technol., 2015, 37(6), 651–657.
- Khan, M. S. and Zaidi, A., Synergistic effects of the inoculation with plant growth promoting rhizobacteria and an arbuscular mycorrhizal fungus on the performance of wheat. Turk. J. Agric. For., 2007, 31, 355–362.
- Roos, W. and Luckner, M., Relationships between proton extrusion and fluxes of ammonium ions and organic acids in Penicillium cyclopium. J. Gen. Microbiol., 1984, 130, 1007–1014.
- Park, K. H., Lee, C. Y. and Son, H. J., Mechanism of insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 isolated from ginseng rhizosphere and its plant growth-promoting activities. Lett. Appl. Microbiol., 2009, 49, 222–228.
- Aspiras, R. B., Allen, O. N., Harris, R. F. and Chesters, G., The role of microorganisms in the stabilization of soil aggregates. Soil. Biol. Biochem., 1971, 3, 347–353.
- Ionescu, M. and Belkin, S., Overproduction of exopolysaccharides by an Escherichia coli K-12 rpoS mutant in response to osmotic stress. Appl. Environ. Microbiol., 2009, 75, 483–492.
- Vassilev, N., Vassileva, M. and Niklaeval, I., Simultaneous Psolubilizing and biocontrol activity of microorganisms potential and future trends. Appl. Microbiol. Biotechnol., 2006, 71, 137–144.
- Caballero-Mellado, J., Onofre-Lemus, J., Estrada-de los, S. and Martinez-Aguilar, L., The tomato rhizosphere, an environment rich in nitrogenfixing Burkholderia species with capabilities of interest for agriculture and biorememdiation. Appl. Environ. Microbiol., 2007, 73, 5308–5319.
- Hamdali, H., Bouizgame, B., Hafidi, M., Lebrihi, A., Virolle, M. J. and Ouhdouch, Y., Screening for rock phosphate solubilizing Actinomycetes from Moroccon phosphate mines. Appl. Soil. Ecol., 2008, 38, 12–19.
- Ehrlich, H. L., Newman, D. K. and Kappler, A., Geomicrobiology, Taylor & Francis Group, Boca Raton, 2016.
- Nannipieri, P., Giagnoni, L., Landi, L. and Renella, G., Role of phosphatase enzymes in soil. In Phosphorus in Action: Biological Processes in Soil Phosphorus Cycling, Springer, Heidelberg, 2011.
- Eivazi, F. and Tabatabai, M. A., Phosphatases in soils. Soil. Biol. Biochem., 1977, 9, 167–172.
- Jorquera, M. A., Hernandez, M. T., Rengel, Z., Marschner, P. and Mora, M. D., Isolation of culturable phosphobacteria with both phytate-mineralization and phosphate-solubilization activity from the rhizosphere of plants grown in a volcanic soil. Biol. Fertil. Soils., 2008, 44, 1025–1034.
- Wanner, B. L., Phosphorus assimilation and control of the phosphate regulon. Escherichia coli and Salmonella: cellular and molecular biology. ASM Press, Washington, DC, 1996, vol. 41, pp. 1357–1381.
- Patel, B. B., Patel, B. B. and Dave, R. S., Studies on infiltration of saline-alkali soils of several parts of Mehsana and Patan districts of north Gujrat. J. Appl. Technol. Environ. Sanitation, 2011, 1(1), 87–92.
- Yamaguchi, T. and Blumwald, E., Developing salt tolerant crop plants: challenges and opportunities. Trends Plant Sci., 2005, 10(12), 615–620.
- Shahbaz, M. and Ashraf, M., Improving salinity tolerance in cereals. Crit. Rev. Plant Sci., 2013, 32, 237–249.
- Munns, R., James, R. A. and Lauchli, A., Approaches to increasing the salt tolerance of wheat and other cereals. J. Exp. Bot., 2006, 57(5), 1025–1043.
- Johri, J. K., Surange, S. and Nautiyal, C. S., Occurrence of salt, pH, and temperature-tolerant, phosphate solubilizing bacteria in alkaline soils. Curr. Microbiol., 1999, 39, 89–93.
- Nautiyal, C. S., Bhadauria, S., Kumar, P., Lal, H., Mondal, R. and Verma, D., Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiol. Lett., 2000, 182, 291–296.
- Gee, J. M., Lund, B. M., Metcalf, G. and Peel, J. L., Properties of a new group of alkalophilic bacteria. J. Gen. Microbiol., 1980, 117, 9–17.
- Pikovskaya, R. I., Mobilization of phosphorus in soil in connection with vital capacity of source microbial species. Microbiologia, 1948, 17, 362-370.
- Lin, T.-F., Huang, H.-I., Shen, F.-T. and Young, C.-C., The protons of gluconic acid are the major factor responsible for the dissolution of tri-calcium phosphate by Burkholderia cepacia CC-A174. Bioresour. Technol., 2006, 97, 957–960.
- Schwyn, B. and Neilands, J. B., Universal chemical assay for the detection and determination of siderophore. Anal. Biochem., 1987, 160, 47–56.
- Ferreira, A. A., Tette, P. A., Mendonca, R. C. S., Soares, A. D. S. and Carvalho, M. M. D., Detection of exopolysaccharide production and biofilm related genes in Staphylococcus spp. isolated from a poultry processing plant. Food Sci. Technol. (Campinas), 2014, 34(4), 710–716.
- Helianti, I., Okubo, T., Morita, Y. and Tamiya, E., Characterization of thermostable native alkaline phosphatase from an hyperthermophilic archeon Aeropyrum pernix K1. Appl. Microbiol. Biotechnol., 2007, 74, 107–112.
- Laemmli, U. K., Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 1970, 227, 680–685.
- Wray, W., Boulikas, T., Wray, V. P. and Hancock, R., Silver staining of proteins in polyacrylamide gels. Anal. Biochem., 1981, 118(1), 197–203.
- Fling, S. P. and Gregerson, D. S., Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Anal. Biochem., 1986, 155(1), 83–88.
- Mirhadi, B., Mehdikhani, B. and Askari, N., Synthesis of nano-sized beta-tricalcium phosphate via wet precipitation. Proc. Appl. Ceram., 2011, 5(4), 193–198.
- Dean, R. L., Kinetic studies with alkaline phosphatase in the presence and absence of inhibitors and divalent cations. Biochem. Mol. Biol. Educ., 2002, 30, 401–407.
- Lowry, O. H., Rosebrough, M. J., Farr, A. L. and Randall, R. J., Protein measurement with the Folin-phenol reagent. J. Biol. Chem., 1951, 265–275.
- Audipudi, A. V., Pradeep, K. N. and Sudhir, A., Phosphate solubilizing microorganisms associated with Chollangi mangrove soil in east coast of India. Int. J. Sci. Eng. Res., 2012, 3, 2229–5518.
- Pramod, K. C. and Dhevendaran, K., Studies on phosphorus bacteria in Cochin Backwater. J. Mar. Biol. Ass. India, 1987, 29, 297–305.
- Vazquez, P., Holguin, G., Puente, M. E., Lopez-Cortes, A. and Bashan, Y., Phosphate solubilizing microorganisms associated with the rhizosphere of mangrove in a semiarid coastal lagoon. Biol. Fertil. Soils, 2000, 30, 460–468.
- Fankem, H., Ngo, L., Deubel, A., Quinn, J., Mebach, W., Etova, X. F. and Nwaga, D., Solubilization of phosphates and growth promotion by Pseudomonas fluroscens isolated from acidic soils of Cameroon. Afr. J. Microbiol. Res., 2008, 2, 171–178.
- Ok-Ryul, S., Seung-Jin, L., Yong-Seok, L., Sang-Cheol, L., Keun-Ki, K. and Yong-Lark, C., Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil. Braz. J. Microbiol., 2008, 39, 151–156.
- Vyas , P. and Gulati, A., Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomas. BMC Microbiol., 2009, 9, 1–15.
- Hwangbo, H., Park, R., Kim, Y., Rim, Y., Park, K., Kim, T., Suh, J. and Kim, K., 2-Ketogluconic acid production and phosphate solubilization by Enterobacter intermedium. Curr. Microbiol., 2003, 47, 87–92.
- Tripura, C., Sashidhar, B. and Podile, A. R., Ethyl methanesulfonate mutagenesis-enhanced mineral phosphate solubiization by groundnut-associated Serratia marcescens GPS-5. Curr. Microbiol., 2007, 54, 79–84.
- Behera, B. C., Yadav, H., Singh, S. K., Mishra, R. R., Sethi, B. K., Dutta, S. K. and Thatoi, H. N., Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha, India. J. Genet. Eng. Biotechnol., 2017, 15, 1–10.
- Goldstein, A. H., Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by Gram-negative bacteria. Biol. Agric. Hort., 1995, 12, 185–193.
- Bajpai, P. D. and Rao, S., Phosphate solubilizing bacteria. Soil Sci. Plant Nutr., 1971, 17, 46–53.
- Hsu, P.-C. L., Condron, L., O’Callaghan, M. and Hurst, M. R. H., hemX is required for production of 2-kelogluconate the predominant organic anion required for inorganic phosphate solubilization by Burkholderia sp. Ha185. Environ. Microbiol. Rep., 2015, 7(6), 918–928.
- Sharan, A., Shikha and Darmwal, N. S., Efficient phosphorus solubilization by mutant strain of Xanthomonas campestris using different carbon, nitrogen and phosphorus sources. World J. Microbiol. Biotechnol., 2008, 24, 3087–3090.
- Ochoa-Loza, F. J., Artiola, J. F. and Maier, R. M., Stability constants for the complexation of various metals with a rhamnolipid biosurfactant. J. Environ. Q., 2001, 30, 479–485.
- Yi, Y., Huang, W. and Ge, Y., Exopolysaccharide: a novel important factor in the microbial dissolution of tricalcium phosphate. World J. Microbiol. Biotechnol., 2008, 24, 1059–1065.
- Reid, R. K., Reid, C. P. P. and Szaniszlo, P. J., Effects of synthetic and microbially producted chelates on the diffusion of iron and phosphorus to a simulated ischolar_main in soil. Biol. Fertil. Soils, 1985, 1, 42–45.
- Luo, L., Meng, H. and Gu, J.-D., Microbial extracellular enzymes in biogeochemical cycling of ecosystems. J. Environ. Manage, 2017, 539–549.
- Wood, D. A. and Tristram, H., Localization in the cell and extraction of alkaline phosphatase from Bacillus subtilis. J. Bacteriol., 1970, 104, 1045–1054.
- Priest, F. G., Extracellular enzyme sunthesis in the genus Bacillus. Bacteriol. Rev., 1977, 41(3), 711–753.
- Liang, X., Kierans, M., Ceci, A., Hillier, S. and Gadd, G. M., Phosphatase mediated bioprecipitation of lead by soil fungi. Environ. Microbiol., 2016, 18, 219–231.
- Tsekova, K., Galabova, D. and Todorova, K., Copper accumulation and phosphatase activities of Aspergillus and Rhizopus. Z. Naturforsch., 2000, 55, 708–712.
- Hauksson, J. B., Andresson, O. S. and Asgeirsson, B., Heat-labile bacterial alkaline phosphatase from a marine Vibrio sp. Enzyme Microb. Technol., 2000, 27, 66–73.
- Halford, S. E., Escherichia coli alkaline phosphatase. Biochem. J., 1971, 125, 319–327.
- Aziz, I., Rashid, N., Ashraf, R., Bashir, Q., Imanaka, T. and Akhtar, M., Pcal_0111, a highly thermostable bifunctional fructose-1,6-biphosphate aldolase/phosphatase from Pyrobaculum calidifontis. Extremophiles, 2017, 21, 513–521.
- Hou, E., Chen, C., Wen, D. and Liu, X., Phosphatase activity in relation to key litter and soil properties in mature subtropical forests in China. Sci. Total Environ., 2015, 515, 83–91.
- Dimroth, P., Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria. Microbiol. Rev., 1987, 51, 320–340.