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Kirubagaran, R.

Detection of IHHN Virus in a Specific Pathogen Free Bonafide Penaeus vannamei (Boone, 1931) Broodstock Consignment Imported to India

Abstract Views :322 | PDF Views:77

Authors

M. C. Remany ¹, R. Kirubagaran ², Daly Cyriac ¹, O. C. Sruthi Prem ¹, P. Krishnakanth Varadaraju ¹, A. K. Panda ¹, Jaideep Kumar ³, Y. C. Thampi Samraj ³

Affiliations
1 Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, IN
2 National Institute of Ocean Technology, Palli Karanai, Chennai 600 100, IN
3 Rajiv Gandhi Centre for Aquaculture, Technology Transfer Training and Administrative Complex, MPEDA (Ministry of Commerce and Industry, Govt of India), 3/197, Poompuhar Road, Karaimedu, Sattanathapuram 609 109, IN

Source

Current Science, Vol 114, No 01 (2018), Pagination: 196-200

Abstract

The World Organization for Animal Health (formerly Office International des Epizooties OIE) listed pathogenic shrimp virus, infectious hypodermal haematopoetic necrosis virus (IHHNV), has been detected in a specific pathogen free (SPF) declared consignment imported from an approved SPF vannamei broodstock supplier to India. The Aquatic Quarantine Facility (AQF), Chennai the only Government-approved quarantine premise for ensuring the SPF status of imported Penaeus vannamei broodstock detected this pathogen during its routine screening process. The AQF, which has so far screened about 1068 SPF batches of vannamei consignment, recorded that one particular batch imported to India was not SPF, though the exporting country declared the stock as SPF. The presence of the viral pathogen was confirmed by both nested PCR and quantitative PCR (qPCR). Sequencing data of the target viral genome revealed 100% similarity with the already published decapod penstyldensovirus-1 isolate IHHNV partial genome (GenBank accession no. KJ862253). The test results declared by AQF were further validated by the Central Institute of Brackishwater Aquaculture, Chennai, which is the referral laboratory of AQF. As the import of non-SPF stock into the country is a source of biosecurity threat, the entire batch was incinerated according to the standard operating procedure of AQF. Thus the stringent biosecurity measures and disease diagnostic strategies followed at AQF served to safeguard the industry from this virus, which would have otherwise gained entry into the country through the falsely claimed SPF stock. This communication highlights the importance of SPF stock and detection of IHHNV.

Keywords

Bonafide, Shrimp Aquaculture, Broodstock, Infectious Hypodermal Haematopoetic Necrosis Virus, Specific Pathogen Free.

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References

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Production and Characterization of Antimicrobial Peptides from Bacillus subtilis Isolated from Deep-Sea Core Samples

Abstract Views :235 | PDF Views:82

Authors

A. Ganesh Kumar ¹, G. Dharani ¹, R. Kirubagaran ¹, M. A. Atmanand ¹

Affiliations
1 Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, IN

Source

Current Science, Vol 118, No 11 (2020), Pagination: 1725-1730

Abstract

A new strain of Bacillus subtilis isolated from deep-sea core sediment sample (1400 m depth) produced antimicrobial peptides (AMPs) when cultured at 50 and 100 bar pressure conditions. The minimum inhibitory concentrations (MIC) showed that the AMPs had potent activity against V. cholerae and K. pneumoniae. AMPs extracted from cells grown at ambient and elevated pressure conditions exhibited distinct antifungal and antibacterial activities. Analysis of genes encoding AMPs revealed the presence of srfAA, sbo and bmyB biosynthetic genes. GC–MS analysis confirmed substantial accumulation of unsaturated fatty acids in membrane lipids of the cells in response to elevated pressure.

Keywords

Antimicrobial Peptides, Bacillus subtilis, Biosynthetic Genes, Deep-Sea Bacteria, Piezotolerance.

Full Text

References

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Mondol, M. A., Shin, H. J. and Islam, M. T., Diversity of secondary metabolites from marine Bacillus species: chemistry and biological activity. Mar. Drugs, 2013, 11, 2846–2872.

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Mass Culture of Marine Microalgae Chlorella vulgaris (NIOT-74) and Production of Biodiesel

Abstract Views :224 | PDF Views:92

Authors

G. Dharani ¹, D. Magesh Peter ¹, J. T. Mary Leema ¹, T. S. Kumar ¹, K. Thirupathi ¹, A. Josephine ¹, R. Kirubagaran ¹, M. A. Atmanand ¹

Affiliations
1 National Institute of Ocean Technology, Ministry of Earth Sciences, Chennai 600 100, IN

Source

Current Science, Vol 118, No 11 (2020), Pagination: 1731-1738

Abstract

Biodiesel production using marine microalgae as an alternate fuel source is receiving international attention in view of its economic and environmental advantages. The present study evaluated the feasibility of biodiesel production from the marine microalgae; Chlorella vulgaris (NIOT-74). Outdoor mass cultures of marine microalgae were done in different photobioreactors and raceways with marine C. vulgaris (NIOT- 74) as a model organism. The study demonstrated the feasibility of producing biodiesel and provided an evaluation of the physico-chemical properties of biodiesel (B100) and blend (B10) according to ASTM standards. A cost-effective electroflocculation method with 90.12% harvesting efficiency was developed and tested. The biodiesel produced from C. vulgaris (NIOT-74) was tested in two-stroke and four-stroke engines and was also used to test drive a vehicle.

Keywords

Biodiesel, Chlorella vulgaris, Fuel Properties, Photobioreactors.

Full Text

References

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Optimization of Algal Culture Medium for Zeaxanthin Production by Dunaliella tertiolecta: An RSM based Approach

Abstract Views :270 | PDF Views:85

Authors

S. Priyanka ¹, R. Kirubagaran ¹, J. T. Mary Leema ¹

Affiliations
1 Department of Marine Biotechnology, National Institute of Ocean Technology, Chennai 600 100, IN

Source

Current Science, Vol 119, No 12 (2020), Pagination: 1997-2005

Abstract

The Chlorophycean microalgae Dunaliella have gained commercial interest because of the synthesis of highly valuable products. One among them is zeaxanthin, a xanthophyll carotenoid valued for its nutraceutical potential related to prevention of age-related macular degeneration and cataract, which is the primary cause for blindness. To improve zeaxanthin production by the microalgae Dunaliella tertiolecta (NIOT-141), De Walne’s medium was optimized to its most favourable nutrient level using response surface methodology (RSM) approach. Plackett–Burman method was employed to screen the most significant nutrients influencing zeaxanthin accumulation which revealed sodium nitrate, trace metals and sodium dihydrogen phosphate as the crucial medium components for increasing zeaxanthin production (P < 0.05). Further, RSM was employed to study the interaction between these factors and identify optimum concentration of the significant ingredients for higher zeaxanthin production. The highest zeaxanthin production reached 20.2 ± 1.29 mg l^–1 under the optimal conditions of 910 mg l^–1 NaNO₃, 40.5 mg l^–1 NaHM₂PO₄ and 0.605 mg l^–1 trace metals solution. Moreover, validation of optimized medium resulted in a three-fold increase in zeaxanthin production compared to unoptimized Walne’s medium (6.72 ± 0.22 mg l^–1). The results thus obtained were more analogous to the predicted values. Hence the model is effective for enhancing zeaxanthin production in D. tertiolecta.

Keywords

Dunaliella tertiolecta, De Walne’s Medium, Nutraceutical, Response Surface Methodology, Zeaxanthin.

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