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Hoi, Pham Xuan
- Identification and Characterization of a Stress-Inducible Gene OsNLI-IF Enhancing Drought Tolerance in Transgenic Tobacco
Abstract Views :201 |
PDF Views:79
Authors
Affiliations
1 Institute of Agricultural Genetics, Pham Van Dong Road, Tu Liem District, Hanoi, VN
2 International Centre for Genetic Engineering and Biotechnology, ICGEB Campus, Aruna Asaf Ali Marg, P.O. Box 10504, New Delhi 110 067, IN
3 VNU University of Science, No. 334 Nguyen Trai Road, Thanh Xuan District, Hanoi, VN
1 Institute of Agricultural Genetics, Pham Van Dong Road, Tu Liem District, Hanoi, VN
2 International Centre for Genetic Engineering and Biotechnology, ICGEB Campus, Aruna Asaf Ali Marg, P.O. Box 10504, New Delhi 110 067, IN
3 VNU University of Science, No. 334 Nguyen Trai Road, Thanh Xuan District, Hanoi, VN
Source
Current Science, Vol 109, No 3 (2015), Pagination: 541-551Abstract
Plants respond to the adverse environment by activat-ing a series of stress-inducible genes, including genes encoding transcription factors. The expression of these genes is regulated by a core DNA sequence con-tained in their promoter region called cis-acting element. The promoter region of several stress-responsive genes contains several stress-regulated cis-acting elements such as dehydration-responsive element, C-repeat, low-temperature-responsive element, NAC recognition sequence and ZFHD recognition sequence. In this study we isolated a cDNA for a transcription factor named nuclear LIM interactor-interacting fac-tor from rice cDNA library by yeast one-hybrid screening using two target sequences of 50 nucleotides derived from two stress-inducible promoters, JRC0528 and JRC0332, of cold-inducible genes OsZF1 and OsNAC6 respectively, as baits. The NLI-IF protein showed both DNA-binding and transcriptional activities in yeast experiments. Expression of OsNLI-IF was found to be induced by cold, heat, salt and drought stresses. The OsNLI-IF gene overexpressing transgenic tobacco plants showed improvement in drought tolerance. The present study emphasizes that OsNLI-IF could be useful for development of drought-tolerant transgenic crop plants.Keywords
Rice, Stress-Inducible Gene, Transcription Factor, Transgenic Tobacco.Full Text
- Isolation and Characterization of a OsRap2.4A Transcription Factor and its Expression in Arabidopsis for Enhancing High Salt and Drought Tolerance
Abstract Views :298 |
PDF Views:100
Authors
Affiliations
1 Department of Plant Molecular Pathology and Abiotic Stress, Institute of Agricultural Genetics, Pham Van Dong Road, Tu Liem District, Hanoi, VN
1 Department of Plant Molecular Pathology and Abiotic Stress, Institute of Agricultural Genetics, Pham Van Dong Road, Tu Liem District, Hanoi, VN
Source
Current Science, Vol 108, No 1 (2015), Pagination: 51-62Abstract
Dehydration responsive element/C-repeat (DRE/CRT) is a cis-acting element involved in the regulation of abiotic stress-responsive gene expression in higher plants. Using a 50-nucleotide bait containing DRE cis-acting element localized on the downstream mini TATA box of glutamate dehydrogenase-like protein (JRC2606) promoter in yeast one-hybrid screening, we have identified two transcription factors belonging to A6 subgroup of DREB subfamily - OsRap2.4A and OsRap2.4B. Expression of OsRap2.4A was induced by drought, high salt and temperature stress conditions, and also by abscisic acid treatment. Binding assay showed that OsRap2.4A bound specifically to DRE sequence in both in vivo and in vitro experiments. Transient expression of OsRap2.4A in protoplasts revealed that OsRap2.4A functioned as a transcriptional activator and upregulated expression of the GUS re-porter gene. Transgenic Arabidopsis plants expressing OsRap2.4A at low level showed no significant growth re-tardation under normal condition, whereas at high level expression of OsRap2.4A caused an obvious retardation. Expression of OsRap2.4A showed the tolerance against drought and salt stresses in comparison with control. These results suggested that OsRap2.4A gene may be involved in a new regulatory pathway in plant responses to abiotic stresses and it is potentially useful for the transformation into crop plants to improve tolerance against drought and high salt stresses.Keywords
Abiotic Stress, AP2 Domain, Arabidopsis Plants, Dre Element, Transcriptional Repressor.Full Text
- Biological Control of Potato Tuber Soft Rot using N-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria
Abstract Views :225 |
PDF Views:75
Authors
Nguyen Thanh Ha
1,
Tran Quang Minh
2,
Pham Xuan Hoi
1,
Nguyen Thi Thanh Thuy
3,
Naruto Furuya
4,
Hoang Hoa Long
1
Affiliations
1 Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, VN
2 Soils and Fertilizers Research Institute, Dong Ngac, Tu Liem, Hanoi, VN
3 Ministry of Agriculture and Rural Development, Ngoc Ha, Ba Dinh, Hanoi, VN
4 Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, JP
1 Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, VN
2 Soils and Fertilizers Research Institute, Dong Ngac, Tu Liem, Hanoi, VN
3 Ministry of Agriculture and Rural Development, Ngoc Ha, Ba Dinh, Hanoi, VN
4 Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, JP
Source
Current Science, Vol 115, No 10 (2018), Pagination: 1921-1927Abstract
Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103–104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.Keywords
AHL, Pectobacterium carotovorum, Potato Tuber Soft Rot, Quorum Sensing, Quorum Quenching.Full Text
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