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Selokar, Naresh L.
- CRISPR-Cas:The Molecular Scissors for Genetic Disease Surgeries?
Abstract Views :270 |
PDF Views:78
Authors
Affiliations
1 Department of Biotechnology, Friedrich-Loeffler-Institute, Mariensee, 31535 Neustadt, DE
1 Department of Biotechnology, Friedrich-Loeffler-Institute, Mariensee, 31535 Neustadt, DE
Source
Current Science, Vol 115, No 3 (2018), Pagination: 383-385Abstract
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is an emerging biomedical tool, which allows researchers to manipulate DNA sequence(s) for desired gene(s) function. Genetic disorders, namely sickle-cell anaemia and β-thalassemia, had corrected in the stem cells using CRISPR. Therefore, researchers are much optimistic that CRISPR can prevent and cure human diseases in the coming future. However, it is a debatable question that whether this tool is safe and effective for use in human.Full Text
References
- Cong, L. et al., Science, 2013, 339, 819–823.
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- Liang, P. et al., Protein Cell, 2017, 8, 811.
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- National Guidelines for Stem Cell Research, Indian Council of Medical Research, Delhi, 2017; icmr.nic.in/guidelines/Guidelines_for_stem_cell_research_2017.pdf
- Simple Somatic Cell Assay to Screen Mammalian Embryo Toxicity Caused by Mineral Oil
Abstract Views :259 |
PDF Views:71
Authors
Rasika Rajendran
1,
Monika Saini
1,
Seema Dua
1,
Diksha Saini
1,
Dharmendra Kumar
1,
Prem Singh Yadav
1,
Naresh L. Selokar
1
Affiliations
1 Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, IN
1 Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, IN
Source
Current Science, Vol 117, No 8 (2019), Pagination: 1270-1271Abstract
The success of in vitro fertilization (IVF), mammalian embryo culture and somatic cell nuclear transfer (SCNT) depend on stringent laboratory conditions and quality of chemicals, plastic ware, and glassware used to culture generated embryos. Therefore, all components and conditions need to be regularly monitored to avoid any kind of contamination and toxicity. Mineral oil (MO), a petroleum product, is extensively used to overlay the embryo cultures to maintain pH and osmolality of the culture medium. In human and farm animals, embryos are cultured with MO for 5 to 7 days to develop fertilized embryos to blastocyst stage1. Commercial suppliers claimed that MO is embryo safe by testing it with mouse embryo culture; however, MO quality can varied by a lot number and may deteriorate by improper storage and transport conditions. In addition, MO is an oil product that makes it susceptible to peroxidation and free radical formation2,3. Therefore, embryologist( s) should be more attentive to avoid MO-based embryo contamination and toxicity.Full Text
References
- Tae, J. C. et al., J. Assist. Reprod. Genet., 2006, 23, 121–127.
- Otsuki, J., Nagai, Y. and Chiba, K., Fertil. Steril., 2007, 88(3), 741–743.
- Morbeck, D. E. and Leonard, P. H., Methods Mol. Biol., 2012, 912, 325– 331.
- Hughes, P. M. et al., J. Assist. Reprod. Genet., 2010, 27(2–3), 87–92.
- Morbeck, D. E., Semin. Reprod. Med., 2012, 30(3), 182–190.
- Selokar, N. L. et al., Theriogenology, 2012, 78(4), 930–936.
- Sadeesh, E. M. et al., Cytotechnology, 2016, 68(5), 1973–1986.
- Selokar, N. L. et al., Cell Reprogram., 2018, 20(3), 157–163.