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Dna Barcoding of the Protected Horned Helmet, Cassis cornuta (Linnaeus 1758)†


Affiliations
1 Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India
 

The horned helmet Cassis cornuta (Linnaeus 1758) is the largest of all helmet shells belonging to the family Cassidae. In India, C. cornuta is protected under the Wildlife Protection Act, 1972 (Schedule- I, Part IV-B) due to its conservation importance. Also, it is one of the most sought after tropical marine molluscs in illegal trading. In the present study, we have performed DNA barcoding for this species using the mitochondrial marker gene, cytochrome c oxidase subunit 1 (COI), and deposited the data at GenBank (accession no. MK878541). The evolutionary history was inferred adopting the maximum likelihood method and Kimura 2-parameter model by encompassing representative organisms belonging to the genus Cassis. As C. cornuta is in great demand among shell collectors and is illegally traded across countries, the DNA barcode data available in the public database would provide an additional checkpoint in reducing the chance of unlawful trade of this shell. Further, it strengthens conservation management practices, particularly at the port of entries where portable DNA barcoding facilities are in practice.

Keywords

Cassis cornuta, COI Gene, DNA Barcoding, Protected Species.
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  • Dna Barcoding of the Protected Horned Helmet, Cassis cornuta (Linnaeus 1758)†

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Authors

B. Vignesh Kumar
Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India
J. V. Anisha Shafni
Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India
V. Deepak Samuel
Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India
K. R. Abhilash
Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India
R. Purvaja
Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India
R. Ramesh
Conservation of Coastal and Marine Resources Division, National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change, Government of India, Anna University Campus, Chennai 600 025, India

Abstract


The horned helmet Cassis cornuta (Linnaeus 1758) is the largest of all helmet shells belonging to the family Cassidae. In India, C. cornuta is protected under the Wildlife Protection Act, 1972 (Schedule- I, Part IV-B) due to its conservation importance. Also, it is one of the most sought after tropical marine molluscs in illegal trading. In the present study, we have performed DNA barcoding for this species using the mitochondrial marker gene, cytochrome c oxidase subunit 1 (COI), and deposited the data at GenBank (accession no. MK878541). The evolutionary history was inferred adopting the maximum likelihood method and Kimura 2-parameter model by encompassing representative organisms belonging to the genus Cassis. As C. cornuta is in great demand among shell collectors and is illegally traded across countries, the DNA barcode data available in the public database would provide an additional checkpoint in reducing the chance of unlawful trade of this shell. Further, it strengthens conservation management practices, particularly at the port of entries where portable DNA barcoding facilities are in practice.

Keywords


Cassis cornuta, COI Gene, DNA Barcoding, Protected Species.



DOI: https://doi.org/10.18520/cs%2Fv119%2Fi12%2F2014-2019