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Antimicrobial Activity of Indian Meal Moth Silk, Plodia interpunctella


Affiliations
1 University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia
 

In this study, inhibitory effects of crude silk produced by 5th larval instar of moth Plodia interpunctella, as well as ethanol, acetone, ethyl acetate, hexane and dichloromethane silk extracts were evaluated against 18 bacterial strains (ATCC strains/isolates) and 6 fun-gal ATCC strains in vitro. Among the tested extracts, the acetone silk extract had the highest activity against Gram-negative bacteria,while all the extracts showed moderate to high activity against Gram-positive bacteria and fungi. The dilution of 1/2, 1/4 of stock samples showed higher microbial activity. The crude silk was not bioactive. This is the first report on antimicrobial activity of different extracts of silk of P. interpunctella larvae. These results open new ave-nues for future research in using this major storage insect pest in biomedical applications.

Keywords

Antimicrobial Activity, Bacteria, Fungi, Insect Pests And Silken Web.
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  • World Health Organization (WHO), Antimicrobial resistance, 2018; https://www.who.int/antimicrobial-resistance/en/

  • Badiee, P. and Hashemiyadeh, Z., Opportunistic invasive fungal infection: diagnosis and clinical management. Indian J. Med. Res., 2014, 139, 195–204.

  • Ullah, H. and Ali, S., Classification of anti‐bacterial agents and their functions. In Antibacterial Agents (ed. Kumavath, R. N.), IntechOpen, London, 2017, pp. 1–16.

  • Kosanić, М. and Ranković, B., Lichen secondary metabolites as potential antibiotic agents. In Lichen Secondary Metabolites Bio-active Properties and Pharmaceutical Potential (ed. Ranković, B.), Springer International Cham Heidelberg, New York, Dor-drecht, London, 2015, pp. 81–104.

  • Stefanović, O., Radojević, I., Vasić, S. and Čomić, Lj., Antibacterial activity of naturally occurring compounds from selected plants. In Antimicrobial Agents (ed. Bobbarala, V.), IntechOpen, Croatia, 2012, pp. 1–24.

  • Venturini, M. E., Rivera, C. S., Gonzalez, C. and Blanco, D., Antimicrobial activity of extracts of edible wild and cultivated mushrooms against foodborne bacterial strains. J. Food Protect., 2008, 71, 1701–1706.

  • Rahnamaeian, M. et al., Insect antimicrobial peptides show potentiating functional interactions against Gram-negative bacteria. Proc. Biol. Sci., 2015, 282, 1–10.

  • Korayem, A. M. et al., A Drosophila salivary gland mucin is also expressed in immune tissues: evidence for a function in coagulation and the entrapment of bacteria. Insect Biochem. Mol. Biol., 2004, 34, 1297–1304.

  • Korayem, A. et al., Evidence for an immune function of lepidopte-ran silk proteins. Biochem. Biophys. Res. Commun., 2007, 352, 317–322.

  • Shaik, A. H. and Sehnal, F., Hemolin expression in the silk glands of Galleria mellonella in response to bacterial challenge and prior to cell disintegration. J. Insect Physiol., 2009, 55, 781–787.

  • Sutherland, D. T., Young, J. H., Weisman, S., Hayashi, C. Y. and Merritt, D. J., Insect silk: one name, many materials. Annu. Rev.Entomol., 2010, 55, 171–188.

  • Fedič, R., Žurovec, M. and Sehnal, F., The silk of Lepidoptera. J.Insect Biotechnol. Sericol., 2002, 71, 1–15.

  • Fedič, R., Žurovec, M. and Sehnal, F., Correlation between fibroin amino acid sequence and physical silk properties. J. Biol. Chem., 2003, 278, 35255–35264.

  • Dossey, A. T., Insects and their chemical weaponry: new potential for drug discovery. Nat. Prod. Rep., 2010, 27, 1737–1757.

  • Cherniack, P. E., Bugs as drugs, Part 1: Insects. The ‘new’ alternative medicine for the 21st century? Altern. Med. Rev., 2010, 15, 124–135.

  • Ratcliffe, N., Azambuja, P. and Brasileiro, M. C., Recent advances in developing insect natural products as potential modern day medicines. Evid-Based Compl. Alt. Med., 2014, p. 21; Article ID 904958.

  • Nwekwo, W. C., Antimicrobial activity of silk fibroin microparticles. Ph D thesis, Graduate School of Applied Sciences, Near East University, Nicosia, 2015.

  • Kwon, M. G. et al., Isolation and analysis of natural compounds from silkworm pupae and effects of its extracts on alcohol detoxification. Entomol. Res., 2012, 42, 55–62.

  • Predojević, D. Z., Vukajlović, F. N., Tanasković, S. T., Gvozdenac, S. M. and Pešić, S. B., Influence of maize kernel state and type on life history of Plodia interpunctella (Lepidoptera: Pyralidae). J. Stored Prod. Res., 2017, 72, 121–127.

  • Gvozdenac, S. M. et al., Life history of Plodia interpunctella Hübner on sunflower seeds: Effects of seed qualitative traits and the initial seed damage. J. Stored Prod. Res., 2018, 79, 89–97.

  • Vukajlović, F. N. et al., Life history of Plodia interpunctella (Lepidoptera: Pyralidae) on dried fruits and nuts: effects of macronutrients and secondary metabolites on immature stages.J. Stored Prod. Res., 2019, 83, 243–253.

  • Mohandass, S., Arthur, F. H., Zhu, K. Y. and Throne, J. E., Biology and management of Plodia interpunctella (Lepidoptera: Pyralidae) in stored products. J. Stored Prod. Res., 2007, 43, 302–311.

  • Vasić, S., Vukajlović, F., Radojević, I., Stefanović, O., Pešić, S. and Čomić, Lj., Antimicrobial effects of a silken web produced by the larvae of Plodia interpunctella. International conference on antimicrobial research, Book of abstracts, Madrid, Spain, 2014.

  • Milutinović, M., Čurović, D., Nikodijević, D., Vukajlović, F., Predojević, D., Marković, S. and Pešić, S., The silk of Plodia interpunctella as a potential biomaterial and its cytotoxic effect on cancer cells. Anim. Biotechnol., 2019; doi:10.1080/10495398. 2019.1575848.

  • Silhacek, D. L. and Miller, G. L., Growth and development of the Indian meal moth, Plodia interpunctella (Lepidoptera: Phycitidae) under laboratory mass-rearing conditions. Ann. Entomol. Soc. Am., 1972, 65, 1084–1087.

  • Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard – Ninth Edition. CLSI document M07-A9, Wayne, Pennsylvania, USA, 2012.

  • National Committee for Clinical Laboratory Standards (NCCLS). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard – Second Edition. NCCLS document M27-A2. NCCLS, Wayne, Pennsylvania, USA, 2002.

  • Wang, J., Liu, H., Zhao, J., Gao, H., Zhou, L., Liu, Z., Chen, Y. and Sui, P., Antimicrobial and antioxidant activities of the ischolar_main bark essential oil of Periploca sepium and its main component 2-hydroxy-4-methoxybenzaldehyde. Molecules, 2010, 15, 5807– 5817.

  • Nikaido, H., Molecular basis of bacterial outer membrane permea-bility revisited. Microbiol. Mol. Biol. Rev., 2003, 67, 593–656.


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  • Antimicrobial Activity of Indian Meal Moth Silk, Plodia interpunctella

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Authors

Olgica Stefanovic
University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia
Filip Vukajlovic
University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia
Tamara Mladenovic
University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia
Dragana Predojevic
University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia
Ljiljana Comic
University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia
Snežana B. Pesic
University of Kragujevac, Institute of Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac, Serbia

Abstract


In this study, inhibitory effects of crude silk produced by 5th larval instar of moth Plodia interpunctella, as well as ethanol, acetone, ethyl acetate, hexane and dichloromethane silk extracts were evaluated against 18 bacterial strains (ATCC strains/isolates) and 6 fun-gal ATCC strains in vitro. Among the tested extracts, the acetone silk extract had the highest activity against Gram-negative bacteria,while all the extracts showed moderate to high activity against Gram-positive bacteria and fungi. The dilution of 1/2, 1/4 of stock samples showed higher microbial activity. The crude silk was not bioactive. This is the first report on antimicrobial activity of different extracts of silk of P. interpunctella larvae. These results open new ave-nues for future research in using this major storage insect pest in biomedical applications.

Keywords


Antimicrobial Activity, Bacteria, Fungi, Insect Pests And Silken Web.

References





DOI: https://doi.org/10.18520/cs%2Fv118%2Fi10%2F1609-1614