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Anticandidal Efficiency of Cinnamomum zeylanicum Extracts against Vulvovaginal Candidiasis
The high incidence of Candidal vulvovaginitis (CVV) among pregnant women and its treatment during pregnancy is a challenge as the antifungal therapy is associated with foetal abnormalities. Additionally, various Candida species exhibit resistance to commonly used antifungal agents. Hence, there is a need to develop new therapeutic strategies against CVV. In the present study, we have evaluated the antifungal activity of cinnamon extracted by four solvents with different degrees of polarity against common Candida pathogens. The ethyl acetate extract of cinnamon was the most effective solvent extract and exhibited the highest antifungal activity against Candida albicans, Candid atropicalis and Candida glabrata, with an inhibition zone diameter of 32.47, 32.1 and 16.7 mm respectively. GC-MS revealed that cinnamon ethyl acetate extract comprised of 1-phenylpropene-3.3-diol diacetate (68.5%), eugenol (11.6%), cinnamic acid (8.2%), cinnamaldehyde (6.1%) and 6-ethyl-3,4- dimethylphenol (5.4%). Minimum inhibitory concentration of cinnamon ethyl acetate extract against C. albicans and C. tropicalis was 0.5 mg/disc, while that against C. glabrata was 1 mg/disc. Minimum fungicidal concentration of cinnamon ethyl acetate extract against C. tropicalis was 1 mg/disc, while that against C. albicans and C. glabrata was 2 mg/disc respectively.
Antifungal Activity, Candida, Cinnamomum zeylanicum, Pregnant Women, Vulvovaginitis.
- Sobel, J. D., Recurrent vulvovaginal candidiasis. Am. J. Obstet. Gynecol., 2016, 214(1), 15–21.
- Lopez, A. S., Vaginal Discharge Handbook of Outpatient Medicine, Springer, 2018, pp. 421–436.
- Paulitsch, A., Weger, W., Ginter‐Hanselmayer, G., Marth, E. and Buzina, W. A., 5‐Year (2000–2004) epidemiological survey of Candida and non‐Candida yeast species causing vulvovaginal candidiasis in Graz, Austria. Mycoses, 2006, 49, 471–475.
- Boatto, H. F., Moraes, M. S. D., Machado, A. P., Girão, M. and Fischman, O., Correlação entre os resultados laboratoriais e os sinais e sintomas clínicos das pacientes com candidíase vulvovaginal e relevância dos parceiros sexuais na manutenção da infecção em São Paulo, Brasil. Rev. Bras. Ginecol. Obstet., 2007, 29(2), 80–84.
- Rad, M. M., Zafarghandi, S., Abbasabadi, B. and Tavallaee, M., The epidemiology of Candida species associated with vulvovaginal candidiasis in an Iranian patient population. Eur. J. Obstet. Gynecol. Reprod. Biol., 2011, 155, 199–203.
- Achkar, J. M. and Fries, B. C., Candida infections of the genitourinary tract. Clin. Microbiol. Rev., 2010, 23, 253–273.
- Harriott, M., Lilly, E., Rodriguez, T., Fidel Jr, P. and Noverr, M., Candida albicans forms biofilms on the vaginal mucosa. Microbiology, 2010, 156, 3635–3644.
- Silva, S., Negri, M., Henriques, M., Oliveira, R., Williams, D. W. and Azeredo, J., Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiol. Rev., 2012, 36, 288–305.
- Wächtler, B. et al., Candida albicans–epithelial interactions: dissecting the roles of active penetration, induced endocytosis and host factors on the infection process. PLoS ONE, 2012, 7, e36952.
- Zordan, R. and Cormack, B., Adhesins in opportunistic fungal pathogens (Chapter, 16). In Candida and Candidiasis (eds Calderone, R. A. and Clancy, C. J.), American Society of Microbiology, Washington, USA, 2012, 2nd edn, pp. 243–259.
- Gonçalves, B., Ferreira, C., Alves, C. T., Henriques, M., Azeredo, J. and Silva, S., Vulvovaginal candidiasis: epidemiology, microbiology and risk factors. Crit. Rev. Microbiol., 2016, 42, 905–927.
- Guzel, A. B., Ilkit, M., Akar, T., Burgut, R. and Demir, S. C., Evaluation of risk factors in patients with vulvovaginal candidiasis and the value of chromID Candida agar versus CHROMagar Candida for recovery and presumptive identification of vaginal yeast species. Med. Mycol., 2011, 49, 16–25.
- Nelson, M., Wanjiru, W. and Margaret, M. W., Prevalence of vaginal candidiasis and determination of the occurrence of Candida species in pregnant women attending the antenatal clinic of Thika District Hospital, Kenya. Open J. Med. Microbiol., 2013, 3, 264.
- Johal, H. S., Garg, T., Rath, G. and Goyal, A. K., Advanced topical drug delivery system for the management of vaginal candidiasis. Drug Deliv., 2016, 23, 550–563.
- Dadar, M., Tiwari, R., Karthik, K., Chakraborty, S., Shahali, Y. and Dhama, K., Candida albicans – biology, molecular characterization, pathogenicity, and advances in diagnosis and control – an update. Microb. Pathogenesis, 2018, 117, 128–138.
- Ahmad, A. and Khan, A. U., Prevalence of Candida species and potential risk factors for vulvovaginal candidiasis in Aligarh, India. Eur. J. Obstet. Gynecol. Reprod. Biol., 2009, 144, 68–71.
- Amouri, I. et al., Epidemiological survey of vulvovaginal candidiasis in Sfax, Tunisia. Mycoses, 2011, 54, e499–e505.
- Nurat, A. A., Babalola, G., Shittu, M., Tijani, M. and Adekola, S., Detection and epidemiology of vulvovaginal candidiasis among asymptomatic pregnant women attending a tertiary hospital in Ogbomoso, Nigeria. Int. J. Biomed. Res., 2015, 6, 518–523.
- Grigoriou, O., Baka, S., Makrakis, E., Hassiakos, D., Kapparos, G. and Kouskouni, E., Prevalence of clinical vaginal candidiasis in a university hospital and possible risk factors. Eur. J. Obstet. Gynecol. Reprod. Biol., 2006, 126, 121–125.
- Neerja, J., Aruna, A. and Paramjeet, G., Significance of Candida culture in women with vulvovaginal symptoms. J. Obstet. Gynecol. India, 2006, 56, 139–141.
- Bauters, T. G., Dhont, M. A., Temmerman, M. I. and Nelis, H. J., Prevalence of vulvovaginal candidiasis and susceptibility to fluconazole in women. Am. J. Obstet. Gynecol., 2002, 187, 569– 574.
- Dennerstein, G. J. and Ellis, D. H., Oestrogen, glycogen and vaginal candidiasis. Aust. N. Z. J. Obstet. Gynaecol., 2001, 41, 326–328.
- Špaček, J., Buchta, V., Jílek, P. and Förstl, M., Clinical aspects and luteal phase assessment in patients with recurrent vulvovaginal candidiasis. Eur. J. Obstet. Gynecol. Reprod. Biol., 2007, 131, 198–202.
- Pilmis, B., Jullien, V., Sobel, J., Lecuit, M., Lortholary, O. and Charlier, C., Antifungal drugs during pregnancy: an updated review. J. Antimicrob. Chemother., 2014, 70, 14–22.
- Arendrup, M. C. and Patterson, T. F., Multidrug-resistant Candida: epidemiology, molecular mechanisms, and treatment. J. Infect. Dis., 2017, 216, S445–S451.
- Colombo, A. L., Júnior, J. N. and Guinea, J., Emerging multidrugresistant Candida species. Curr. Opin. Infect. Dis., 2017, 30, 528– 538.
- Serra, E., Hidalgo-Bastida, L., Verran, J., Williams, D. and Malic, S., Antifungal activity of commercial essential oils and biocides against Candida albicans. Pathogens, 2018, 7, 15.
- Chouhan, S., Sharma, K. and Guleria, S., Antimicrobial activity of some essential oils – present status and future perspectives. Medicines (Basel), 2017, 4, 58.
- Li, Y., Nie, Y., Zhou, L., Li, S., Tang, X., Ding, Y. and Li, S., The possible mechanism of antifungal activity of cinnamon oil against Rhizopus nigricans. J. Chem. Pharm. Res., 2014, 6, 12–20.
- Wang, S. Y., Chen, P. F. and Chang, S. T., Antifungal activities of essential oils and their constituents from indigenous cinnamon (Cinnamomum osmophloeum) leaves against wood decay fungi. Bioresour. Technol., 2005, 96, 813–818.
- Cheng, S. S., Liu, J. Y., Chang, E. H. and Chang, S. T., Antifungal activity of cinnamaldehyde and eugenol congeners against woodrot fungi. Bioresour. Technol., 2008, 99, 5145–5149.
- Xie, Y., Yang, Z., Cao, D., Rong, F., Ding, H. and Zhang, D., Antitermitic and antifungal activities of eugenol and its congeners from the flower buds of Syzgium aromaticum (clove). Ind. Crops Prod., 2015, 77, 780–786.
- Zhang, Z., Yang, T., Mi, N., Wang, Y., Li, G., Wang, L. and Xie, Y., Antifungal activity of monoterpenes against wood white-rot fungi. Int. Biodeterior. Biodegrad., 2016, 106, 157–160.
- Cheng, S. S., Chung, M. J., Lin, C. Y., Wang, Y. N. and Chang, S. T., Phytochemicals from Cunninghamia konishii Hayata act as antifungal agents. J. Agric. Food. Chem., 2011, 60, 124–128.
- Gill, A. O. and Holley, R. A., Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei. Appl. Environ. Microbiol., 2004, 70, 5750–5755.
- de Almeida, R. R., Souto, R. N., Bastos, C. N., da Silva, M. H. and Maia, J. G., Chemical variation in Piper aduncum and biological properties of its dillapiole‐rich essential oil. Chem. Biodivers., 2009, 6, 1427–1434.
- Bang, K. H., Lee, D. W., Park, H. M. and Rhee, Y. H., Inhibition of fungal cell wall synthesizing enzymes by trans-cinnamaldehyde. Biosci. Biotechnol. Biochem., 2000, 64, 1061–1063.
- Wang, C., Zhang, J., Chen, H., Fan, Y. and Shi, Z., Antifungal activity of eugenol against Botrytis cinerea. Trop. Plant Pathol., 2010, 35, 137–143.
- Asdadi, A. et al., Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnuscastus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections. J. Mycol. Med., 2015, 25, e118–e127.
- Pereira, F. G., Marquete, R., Domingos, L. T., Rocha, M. E., FerreiraPereira, A., Mansur, E. and Moreira, D. L., Antifungal activities of the essential oil and its fractions rich in sesquiterpenes from leaves of Casearia sylvestris Sw. An. Acad. Bras. Ciênc., 2017, 89, 2817–2824.
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