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Infusing Microbial Consortia for Enhancing Seed Germination and Vigour in Pigeonpea (Cajanus cajan (L.) Millsp.)


Affiliations
1 Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
2 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
3 Agricultural College and Research Institute, Tamil Nadu Agricultural University, Kudumiyanmalai 622 104, India
 

Use of plant growth promoting bacteria for seed treatment is in trend nowadays as it is beneficial to the plants and environment. But, carrier-based inoculants have short shelf life and difficult to use for large quantities of seed. Therefore, in the present study we used liquid microbial cultures for seed infusion in pigeonpea and assessed their compatibility with seed treating chemicals. The results showed that the soaking of pigeonpea seeds in liquid cultures like pink pigmented facultative methylotroph (PPFM) @ 1 : 100 dilution for 3 h or Rhizobium or phosphobacteria @ 1 : 50 dilution for 4 h have showed increased germination and vigour. In the microbial infused seeds, Rhizobium (13 × 104 cfu g–1 of seed) and phosphobacteria (20 × 104 cfu g–1 of seed) populations observed, were slightly reduced during three months storage. Nevertheless, the population was drastically reduced in PPFM (11 × 104 to 2 × 104 cfu g–1 of seed). Conversely, PPFM has performed better in seed quality enhancement amongst cultures. Also, consortia of Rhizobium @ 1 : 50 dilution + PPFM @ 1 : 100 dilution (1 : 1) for 3 h increased seed vigour with better microbial populations (14 × 104 and 2 × 104 cfu g–1 of seed). Also, seed infusion with PPFM liquid culture @ 1 : 100 dilution for 3 h followed by polymer coating @ 5 ml kg–1 + carbendazim treatment @ 2 g kg–1 of seed recorded increased germination and vigour with the PPFM population of 1 × 104 cfu g–1 of seed.

Keywords

Pigeonpea, PPFM, Phosphobacteria, Rhizobium, Seed Germination, Vigour.
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  • Infusing Microbial Consortia for Enhancing Seed Germination and Vigour in Pigeonpea (Cajanus cajan (L.) Millsp.)

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Authors

K. Raja
Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
R. Anandham
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
K. Sivasubramaniam
Agricultural College and Research Institute, Tamil Nadu Agricultural University, Kudumiyanmalai 622 104, India

Abstract


Use of plant growth promoting bacteria for seed treatment is in trend nowadays as it is beneficial to the plants and environment. But, carrier-based inoculants have short shelf life and difficult to use for large quantities of seed. Therefore, in the present study we used liquid microbial cultures for seed infusion in pigeonpea and assessed their compatibility with seed treating chemicals. The results showed that the soaking of pigeonpea seeds in liquid cultures like pink pigmented facultative methylotroph (PPFM) @ 1 : 100 dilution for 3 h or Rhizobium or phosphobacteria @ 1 : 50 dilution for 4 h have showed increased germination and vigour. In the microbial infused seeds, Rhizobium (13 × 104 cfu g–1 of seed) and phosphobacteria (20 × 104 cfu g–1 of seed) populations observed, were slightly reduced during three months storage. Nevertheless, the population was drastically reduced in PPFM (11 × 104 to 2 × 104 cfu g–1 of seed). Conversely, PPFM has performed better in seed quality enhancement amongst cultures. Also, consortia of Rhizobium @ 1 : 50 dilution + PPFM @ 1 : 100 dilution (1 : 1) for 3 h increased seed vigour with better microbial populations (14 × 104 and 2 × 104 cfu g–1 of seed). Also, seed infusion with PPFM liquid culture @ 1 : 100 dilution for 3 h followed by polymer coating @ 5 ml kg–1 + carbendazim treatment @ 2 g kg–1 of seed recorded increased germination and vigour with the PPFM population of 1 × 104 cfu g–1 of seed.

Keywords


Pigeonpea, PPFM, Phosphobacteria, Rhizobium, Seed Germination, Vigour.

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DOI: https://doi.org/10.18520/cs%2Fv117%2Fi12%2F2052-2058