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Genetic Lineage of Zeugodacus caudatus (Diptera:Tephritidae) Detected with mtCOI Gene Analysis from India


Affiliations
1 Department of Entomology, Veer Kunwar Singh College of Agriculture, Dumraon (Bihar Agricultural University, Sabour), Buxar 802 136, India
2 Department of Entomology, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India
3 ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi 834 010, India
4 Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India
5 Department of Entomology, Dr Kalam Agriculture College, Kishanganj 855 107 (Bihar Agricultural University, Sabour),, India
6 Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 094, India
 

Zeugodacus caudatus (Fabricius) is a pest of cucurbit plants. The present study was conducted to draw the relationship among Indian Z. caudatus populations with the other defined genetic lineage of the species. A total of 18 individuals’ mtCOI gene sequences from 3 populations of India were analysed along with 34 individuals’ mtCOI gene sequences from Malaysia, Indonesia, Thailand and China and generated 14 haplotypes. Phylogenetic study revealed the presence of distinct genetic lineage in Z. caudatus populations collected from India. The genetic distance between three distinct lineages of Z. caudatus was 0.057, 0.055 and 0.018 between Indonesia and Malaysia, India and Indonesia, and India and Malaysia, respectively and also evident from phylogenetic analysis. Further, the mitochondrial cytochrome oxidase I (COI) gene sequences developed in this study will help detection and geographical distribution of new haplotypes and lineages of the species in future.

Keywords

Dacinae, Fruit Fly, Haplotypes, Population Genetics.
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  • Genetic Lineage of Zeugodacus caudatus (Diptera:Tephritidae) Detected with mtCOI Gene Analysis from India

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Authors

Chandra S. Prabhakar
Department of Entomology, Veer Kunwar Singh College of Agriculture, Dumraon (Bihar Agricultural University, Sabour), Buxar 802 136, India
Anil
Department of Entomology, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India
Jaipal S. Choudhary
ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi 834 010, India
Ravi S. Singh
Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India
S. N. Ray
Department of Entomology, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India
Kalmesh Managanvi
Department of Entomology, Dr Kalam Agriculture College, Kishanganj 855 107 (Bihar Agricultural University, Sabour),, India
Mona Kumari
Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur 813 210, India
Ashok B. Hadapad
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 094, India
Ramesh S. Hire
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 094, India

Abstract


Zeugodacus caudatus (Fabricius) is a pest of cucurbit plants. The present study was conducted to draw the relationship among Indian Z. caudatus populations with the other defined genetic lineage of the species. A total of 18 individuals’ mtCOI gene sequences from 3 populations of India were analysed along with 34 individuals’ mtCOI gene sequences from Malaysia, Indonesia, Thailand and China and generated 14 haplotypes. Phylogenetic study revealed the presence of distinct genetic lineage in Z. caudatus populations collected from India. The genetic distance between three distinct lineages of Z. caudatus was 0.057, 0.055 and 0.018 between Indonesia and Malaysia, India and Indonesia, and India and Malaysia, respectively and also evident from phylogenetic analysis. Further, the mitochondrial cytochrome oxidase I (COI) gene sequences developed in this study will help detection and geographical distribution of new haplotypes and lineages of the species in future.

Keywords


Dacinae, Fruit Fly, Haplotypes, Population Genetics.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi8%2F1368-1375