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Simple Somatic Cell Assay to Screen Mammalian Embryo Toxicity Caused by Mineral Oil


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1 Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
 

The success of in vitro fertilization (IVF), mammalian embryo culture and somatic cell nuclear transfer (SCNT) depend on stringent laboratory conditions and quality of chemicals, plastic ware, and glassware used to culture generated embryos. Therefore, all components and conditions need to be regularly monitored to avoid any kind of contamination and toxicity. Mineral oil (MO), a petroleum product, is extensively used to overlay the embryo cultures to maintain pH and osmolality of the culture medium. In human and farm animals, embryos are cultured with MO for 5 to 7 days to develop fertilized embryos to blastocyst stage1. Commercial suppliers claimed that MO is embryo safe by testing it with mouse embryo culture; however, MO quality can varied by a lot number and may deteriorate by improper storage and transport conditions. In addition, MO is an oil product that makes it susceptible to peroxidation and free radical formation2,3. Therefore, embryologist( s) should be more attentive to avoid MO-based embryo contamination and toxicity.
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  • Simple Somatic Cell Assay to Screen Mammalian Embryo Toxicity Caused by Mineral Oil

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Authors

Rasika Rajendran
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
Monika Saini
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
Seema Dua
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
Diksha Saini
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
Dharmendra Kumar
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
Prem Singh Yadav
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India
Naresh L. Selokar
Division of Animal Physiology and Reproduction, ICAR-Central Institute for Research on Buffaloes, Hisar 125 001, India

Abstract


The success of in vitro fertilization (IVF), mammalian embryo culture and somatic cell nuclear transfer (SCNT) depend on stringent laboratory conditions and quality of chemicals, plastic ware, and glassware used to culture generated embryos. Therefore, all components and conditions need to be regularly monitored to avoid any kind of contamination and toxicity. Mineral oil (MO), a petroleum product, is extensively used to overlay the embryo cultures to maintain pH and osmolality of the culture medium. In human and farm animals, embryos are cultured with MO for 5 to 7 days to develop fertilized embryos to blastocyst stage1. Commercial suppliers claimed that MO is embryo safe by testing it with mouse embryo culture; however, MO quality can varied by a lot number and may deteriorate by improper storage and transport conditions. In addition, MO is an oil product that makes it susceptible to peroxidation and free radical formation2,3. Therefore, embryologist( s) should be more attentive to avoid MO-based embryo contamination and toxicity.

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DOI: https://doi.org/10.18520/cs%2Fv117%2Fi8%2F1270-1271