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A Simple Method for the Separation and Detection of Trace Levels of Buprofezin, Flubendiamide and Imidacloprid by NP-HPTLC and RP-HPTLC


Affiliations
1 Department of Biochemistry, Gulbarga University, Kalaburagi - 585106, India
2 Regional Forensic Science Laboratory, Kalaburagi - 585102, India
3 Regional Forensic Science Laboratory, Mysuru - 570010, India
 

A study was undertaken to evaluate the retention (RF and RM) and separation (ΔRF, RαF , α and RS) of buprofezin (B), flubendiamide (F) and imidacloprid (I) using n-hexane-acetone (6.5 : 3.5 v/v) in the case of NPHPTLC and methanol-water (8 : 2 v/v) for RP-HPTLC as mobile phase. The study revealed that increasing the acetone content in NP-HPTLC and decreasing the water content in RP-HPTLC resulted in high resolution with increase in RF values for B, F and I. ΔRF > 0.04 and RS > 1.5 were achieved for all pairs of compounds (ΔRF(B–F) = 0.35, ΔRF(F–I) = 0.19, ΔRF(B–I) = 0.54, RS(B–F) = 4.12, RS(F–I) = 7.34, RS(B–I) = 2.02 using NP-HPTLC; ΔRF(F–B) = 0.23, ΔRF(I–F) = 0.26, ΔRF(I–B) = 0.49, RS(F–B) = 2.63, RS(I–F) = 2.97, RS(I–B) = 5.92 using RP-HPTLC). Imidacloprid was adsorbed strongly on NP-HPTLC layer and buprofezin on RP-HPTLC layer, as indicated by their high RM values. The maximum absorption of UV for B, F and I was found to be 252, 242 and 276 nm respectively. Stability analysis indicated that these compounds were stable up to 6 h in methanol and on the plates (NP-HPTLC and RPHPTLC layers). This protocol is useful for toxicologists to detect a mixture of these insecticides in forensic as well as environmental samples.

Keywords

Detection and Separation, Human Toxicity, Insecticides, Thin-Layer Chromatography.
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  • A Simple Method for the Separation and Detection of Trace Levels of Buprofezin, Flubendiamide and Imidacloprid by NP-HPTLC and RP-HPTLC

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Authors

B. Nareshkumar
Department of Biochemistry, Gulbarga University, Kalaburagi - 585106, India
Praveen U. Sangnalmath
Regional Forensic Science Laboratory, Kalaburagi - 585102, India
S. Gayatridevi
Regional Forensic Science Laboratory, Mysuru - 570010, India
K. Sreeramulu
Department of Biochemistry, Gulbarga University, Kalaburagi - 585106, India

Abstract


A study was undertaken to evaluate the retention (RF and RM) and separation (ΔRF, RαF , α and RS) of buprofezin (B), flubendiamide (F) and imidacloprid (I) using n-hexane-acetone (6.5 : 3.5 v/v) in the case of NPHPTLC and methanol-water (8 : 2 v/v) for RP-HPTLC as mobile phase. The study revealed that increasing the acetone content in NP-HPTLC and decreasing the water content in RP-HPTLC resulted in high resolution with increase in RF values for B, F and I. ΔRF > 0.04 and RS > 1.5 were achieved for all pairs of compounds (ΔRF(B–F) = 0.35, ΔRF(F–I) = 0.19, ΔRF(B–I) = 0.54, RS(B–F) = 4.12, RS(F–I) = 7.34, RS(B–I) = 2.02 using NP-HPTLC; ΔRF(F–B) = 0.23, ΔRF(I–F) = 0.26, ΔRF(I–B) = 0.49, RS(F–B) = 2.63, RS(I–F) = 2.97, RS(I–B) = 5.92 using RP-HPTLC). Imidacloprid was adsorbed strongly on NP-HPTLC layer and buprofezin on RP-HPTLC layer, as indicated by their high RM values. The maximum absorption of UV for B, F and I was found to be 252, 242 and 276 nm respectively. Stability analysis indicated that these compounds were stable up to 6 h in methanol and on the plates (NP-HPTLC and RPHPTLC layers). This protocol is useful for toxicologists to detect a mixture of these insecticides in forensic as well as environmental samples.

Keywords


Detection and Separation, Human Toxicity, Insecticides, Thin-Layer Chromatography.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi5%2F895-903