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Diversity in a Widely Distributed Dioecious Medicinal Plant, Tinospora cordifolia (Willd.) Miers. ex. Hook F. and Thomas


Affiliations
1 Molecular Biology and Genetics Laboratory, Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
2 Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
3 B2/M91 SBI Colony, Sector B, Janakipuram, Lucknow 226 021, India
 

The nature and extent of diversity in Tinospora cordifolia, a dioecious climber, widely distributed in India, and one of the most important medicinal plants has remained underexplored. The present study reveals wide range of tinosporaside content (0.016–4.523 mg/g), berberine content (0.27–76.9 mg/g) and jaccard distances (0.0341–0.559). The neighbour joining tree, structure simulation and principal coordinate analysis resolved all the accessions into six sub-clusters, four of which were congruent in the analyses. Sub-cluster I uniquely included all male accessions, with above average leaf areas and below average tinosporaside contents with hairy and fibrous leaves. Further, analysis of molecular variance considering three populations showed that maximum variance (87%) was within the population. The result of this preliminary study revealed genetic diversity, population structure in T. cordifolia.

Keywords

AMOVA, Berberine, ISSR, NJ Tree, RAPD, Tinospora, Tinosporaside.
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  • Diversity in a Widely Distributed Dioecious Medicinal Plant, Tinospora cordifolia (Willd.) Miers. ex. Hook F. and Thomas

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Authors

Suchita Lade
Molecular Biology and Genetics Laboratory, Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
Pooja Singh Sikarwar
Molecular Biology and Genetics Laboratory, Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
Md. Akram Ansari
Molecular Biology and Genetics Laboratory, Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
Sayyada Khatoon
Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
Nikhil Kumar
B2/M91 SBI Colony, Sector B, Janakipuram, Lucknow 226 021, India
Hemant Kumar Yadav
Molecular Biology and Genetics Laboratory, Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India
Shirish A. Ranade
Molecular Biology and Genetics Laboratory, Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India

Abstract


The nature and extent of diversity in Tinospora cordifolia, a dioecious climber, widely distributed in India, and one of the most important medicinal plants has remained underexplored. The present study reveals wide range of tinosporaside content (0.016–4.523 mg/g), berberine content (0.27–76.9 mg/g) and jaccard distances (0.0341–0.559). The neighbour joining tree, structure simulation and principal coordinate analysis resolved all the accessions into six sub-clusters, four of which were congruent in the analyses. Sub-cluster I uniquely included all male accessions, with above average leaf areas and below average tinosporaside contents with hairy and fibrous leaves. Further, analysis of molecular variance considering three populations showed that maximum variance (87%) was within the population. The result of this preliminary study revealed genetic diversity, population structure in T. cordifolia.

Keywords


AMOVA, Berberine, ISSR, NJ Tree, RAPD, Tinospora, Tinosporaside.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi07%2F1520-1526