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Molecular Characterization of Ladybird Predators (Coleoptera: Coccinellidae) of Aphid Pests (Homoptera:Aphididae) in North East India


Affiliations
1 Ecology and Biodiversity Laboratories, Department of Zoology, Tripura University, Suryamaninagar 799 022, India
2 Division of Crop Protection, ICAR Research Complex for NEH Region, Umroi Road, Umiam (Barapani) 793 103, India
 

Ladybird beetles are potential and promising biological control agents for the management of insect pests. These insects show variations in biological fitness in diverse habitats and subsequently in term of genotypes. We used cytochrome oxidase I (COI) gene sequences to study within-species genetic variation in four species of ladybird predators, viz. Coccinella transversalis (Fab.), Cheilomenes sexmaculata (Fab.), Micraspis discolor (Fab.) and Anisolemnia dilatata (Fab.) collected from different cultivated habitats of Tripura, North East India. Results of multiple sequence alignments of partial COI gene (553 bp) of mitochondrial origin showed 100% homology among different populations (within species) of three ladybird species. The molecular identity of M. discolor could not be established due to the absence of matching nucleotide sequence for this region of COI gene in the NCBI database. Three of the four populations of Micraspis species showed 100% homology in partial COI gene sequencing, but one representative population showed 52 nucleotide mutations, of which 1 mutation was found to result in the alteration of the codon from valine to isoleucine, and seemed to represent a different Micraspis species previously not known from NE India. This study shows that the three most common species of ladybird predators of aphid pests in NE India are fairly homogenous with respect to the COI gene, but species of Micraspis are genetically diverse and need further studies to address this issue.

Keywords

Aphid Pests, Genetic Variation, Ladybird Beetles, Molecular Characterization.
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  • Blackman, R. L. and Eastop, V. F., Aphids on the World’s Crops: An Identification and Information Guide, John Wiley, UK, 1984.

  • Ganguli, R. N. and Agarwala, B. K., Aphid association of agricultural crops in Tripura, North East India. Indian Agric., 1985, 29(4), 281–287.

  • Hodek, I. and Evans, E. W., Food relationships. In Ecology and Behaviour of the Ladybird Beetles (Coccinellidae) (eds Hodek, I., van Emden, H. F. and Honek, A.), Blackwell Publishing Ltd, London, 2012, pp. 141–274.

  • Agarwala, B. K., Bardhanroy, P., Yasuda, H. and Takizawa, T., Prey consumption and oviposition of the aphidophagous predator Menochilus sexmaculatus (Coleoptera: Coccinellidae) in relation to prey density and adult size. Environ. Entomol., 2001, 30(6), 1182–1187.

  • Dixon, A. F. G., Insect Predator – Prey Dynamics, Ladybird Beetles and Biological Control, Cambridge University Press, Cambridge, UK, 2000.

  • Agarwala, B. K., Das, S. and Senchowdhuri, M., Biology and food relation of Micraspis discolor (F.), an aphidophagous coccinellid in India. J. Aphidol., 1988, 2(1–2), 7–17.

  • Margo, A., Lecompte, E., Magne, F., Hemptinne, J.-L. and Crouau-Roy, B., Phylogeny of ladybirds (Coleoptera: Coccinellidae): are the subfamilies monophyletic? Mol. Phylogenet. Evol., 2010, 54, 833–848.

  • Aruggoda, A. G. B., Shunxiabg, R. and Baoli, Q., Molecular phylogeny of ladybird beetles (Coccinellidae: Coleoptera) inferred from mitochondrial 16S rDNA sequences. Trop. Agric. Res., 2010, 21, 209–217.

  • Fu, J. and Zhang, Y. C., Sequence analysis of mtDNA – COI gene and molecular phylogeny on twenty seven species of coccinellids (Coleoptera: Coccinellidae). Entomotaxonomia, 2006, 28, 179–185.

  • Hebert, P. D. N., Penton, E. H., Burns, J. M., Janzen, D. H. and Hallwachs, W., Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc. Natl. Acad. Sci. USA, 2004, 101, 14812–14817.

  • Behere, G. T., Tay, W. T., Russell, D. A., Heckel, D. G., Appleton, B. R., Kranthi, K. R. and Batterham, P., Mitochondrial DNA analysis of field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) and of its relationship to H. zea. BMC Evol. Biol., 2007, 7, 117.

  • Majerus, M. E. N., Ladybirds, New Naturalist Series No. 81, Harper Collins, London, UK, 1994.

  • Staden, R., Beal, K. F. and Bonfield, J. K., The Staden Package. Methods Mol. Biol., 2000, 132, 115–130.

  • Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G., The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 1997, 24, 4876–4882.

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S., MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol., 2013, 30, 2725–2729.

  • Jukes, T. H. and Cantor, C. R., Evolution of protein molecules. In Mammalian Protein Metabolism (ed. Munro, H. N.), Academic Press, New York, USA, 1969, pp. 21–132.

  • Poorani, J., An annotated checklist of the Coccinellidae (Coleoptera) (excluding Epilachninae) of the Indian region. Orient. Insects, 2002, 36, 307–383.


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  • Molecular Characterization of Ladybird Predators (Coleoptera: Coccinellidae) of Aphid Pests (Homoptera:Aphididae) in North East India

Abstract Views: 245  |  PDF Views: 76

Authors

Santa Ghosh
Ecology and Biodiversity Laboratories, Department of Zoology, Tripura University, Suryamaninagar 799 022, India
G. T. Behere
Division of Crop Protection, ICAR Research Complex for NEH Region, Umroi Road, Umiam (Barapani) 793 103, India
B. K. Agarwala
Ecology and Biodiversity Laboratories, Department of Zoology, Tripura University, Suryamaninagar 799 022, India

Abstract


Ladybird beetles are potential and promising biological control agents for the management of insect pests. These insects show variations in biological fitness in diverse habitats and subsequently in term of genotypes. We used cytochrome oxidase I (COI) gene sequences to study within-species genetic variation in four species of ladybird predators, viz. Coccinella transversalis (Fab.), Cheilomenes sexmaculata (Fab.), Micraspis discolor (Fab.) and Anisolemnia dilatata (Fab.) collected from different cultivated habitats of Tripura, North East India. Results of multiple sequence alignments of partial COI gene (553 bp) of mitochondrial origin showed 100% homology among different populations (within species) of three ladybird species. The molecular identity of M. discolor could not be established due to the absence of matching nucleotide sequence for this region of COI gene in the NCBI database. Three of the four populations of Micraspis species showed 100% homology in partial COI gene sequencing, but one representative population showed 52 nucleotide mutations, of which 1 mutation was found to result in the alteration of the codon from valine to isoleucine, and seemed to represent a different Micraspis species previously not known from NE India. This study shows that the three most common species of ladybird predators of aphid pests in NE India are fairly homogenous with respect to the COI gene, but species of Micraspis are genetically diverse and need further studies to address this issue.

Keywords


Aphid Pests, Genetic Variation, Ladybird Beetles, Molecular Characterization.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi09%2F1755-1759