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Production Optimization of Alkali-Thermo Tolerant Crude Endoglucanase from Funalia leonina by Response Surface Methodology


Affiliations
1 Forest Pathology Division, Forest Research Institute, Dehradun 248 006, India
2 Cellulose and Paper Division, Forest Research Institute, Dehradun 248 006, India
3 Central Pulp and Paper Research Institute, Saharanpur 247 001, India
4 Ch Kaliram Degree College, Nagal, Saharanpur 247 001, India
 

A remarkable yield of an alkali-thermo-tolerant endoglucanase (2.93 IU/ml) was obtained from a white rot fungus, Funalia leonina through fermentation process in solid state, optimized by response surface methodology. The three test variables, viz. pH of medium, incubation temperature and incubation time were optimized as they have significant effect on enzyme production. After solving the model equation, the optimum values of medium pH, incubation temperature and incubation period were found to be 5.8, 34°C and 10 days respectively, for maximum endoglucanase production. The consequence of culture preservation medium on enzyme production capacity was also studied and wheat bran agar medium was found the most suitable medium for culture preservation.

Keywords

Endoglucanase, Funalia leonina, Preservation Medium, Response Surface Methodology.
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  • Production Optimization of Alkali-Thermo Tolerant Crude Endoglucanase from Funalia leonina by Response Surface Methodology

Abstract Views: 216  |  PDF Views: 78

Authors

Varun Kumar
Forest Pathology Division, Forest Research Institute, Dehradun 248 006, India
Nirmal Sudhir Kumar Harsh
Forest Pathology Division, Forest Research Institute, Dehradun 248 006, India
Sanjay Naithani
Cellulose and Paper Division, Forest Research Institute, Dehradun 248 006, India
Bipin Prakash Thapliyal
Central Pulp and Paper Research Institute, Saharanpur 247 001, India
Shrikant Sharma
Ch Kaliram Degree College, Nagal, Saharanpur 247 001, India

Abstract


A remarkable yield of an alkali-thermo-tolerant endoglucanase (2.93 IU/ml) was obtained from a white rot fungus, Funalia leonina through fermentation process in solid state, optimized by response surface methodology. The three test variables, viz. pH of medium, incubation temperature and incubation time were optimized as they have significant effect on enzyme production. After solving the model equation, the optimum values of medium pH, incubation temperature and incubation period were found to be 5.8, 34°C and 10 days respectively, for maximum endoglucanase production. The consequence of culture preservation medium on enzyme production capacity was also studied and wheat bran agar medium was found the most suitable medium for culture preservation.

Keywords


Endoglucanase, Funalia leonina, Preservation Medium, Response Surface Methodology.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi09%2F1750-1754