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Isolation and Structural Elucidation of an Isothiocyanate Compound from Indigofera tinctoria Linn. Extract


Affiliations
1 Department of Bioscience and Biotechnology, Banasthali University, Niwai 304 022, India
 

Indigofera tinctoria is a well-known medicinal plant that possesses several therapeutic activities. Isothiocyanate derivative from hydroethanolic extract of Indigoferatinctoria (HEIT) was isolated by means of chromatographic techniques, i.e. adsorption chromatography,thin layer chromatography and high-pressure liquid chromatography. Structural characterization of isolated compound was done using various spectroscopic techniques (liquid chromatograph–mass spectrometry,1H nuclear magnetic resonance and Fourier transform-infrared spectroscopy) and the possible structure was identified as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulphanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5;m/z 446.70; ITC-1). Maximum yield of ITC-1 was obtained as 22 mg/5 g HEIT with 97% purity.

Keywords

Column Chromatography, HPLC, Indigofera tinctoria, Isothiocynate Compounds, LC–MS.
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  • Isolation and Structural Elucidation of an Isothiocyanate Compound from Indigofera tinctoria Linn. Extract

Abstract Views: 218  |  PDF Views: 72

Authors

Veena Sharma
Department of Bioscience and Biotechnology, Banasthali University, Niwai 304 022, India
Rashmi Singh
Department of Bioscience and Biotechnology, Banasthali University, Niwai 304 022, India

Abstract


Indigofera tinctoria is a well-known medicinal plant that possesses several therapeutic activities. Isothiocyanate derivative from hydroethanolic extract of Indigoferatinctoria (HEIT) was isolated by means of chromatographic techniques, i.e. adsorption chromatography,thin layer chromatography and high-pressure liquid chromatography. Structural characterization of isolated compound was done using various spectroscopic techniques (liquid chromatograph–mass spectrometry,1H nuclear magnetic resonance and Fourier transform-infrared spectroscopy) and the possible structure was identified as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulphanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5;m/z 446.70; ITC-1). Maximum yield of ITC-1 was obtained as 22 mg/5 g HEIT with 97% purity.

Keywords


Column Chromatography, HPLC, Indigofera tinctoria, Isothiocynate Compounds, LC–MS.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi05%2F941-946