Asian Journal of Research in Pharmaceutical Sciences

Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

Two spectrophotometric methods are described for determination of Clarithromycin and Clindamycin in bulk and pharmaceutical dosage forms using two acidic dyes, bromocresol purple and Bromocresol green to form ion pair extractable complexes with clarithromycin and clindamycin HCl then measuring absorbances at (390nm for clarithromycin or 397 nm for clindamycin) and 413 nm respectively. The effect,s of acidity, buffer volume and dye concentration, on the absorption were studied. Calibration curves were linear over ranges of 12-28 μg.ml-1 for Clarithromycin and 8- 40 μg.ml-1 for Clindamycin in case of bromocresol purple and of 4-20 μg.ml-1 for Clarithromycin and 16-32μg.ml-1 for Clindamycin in case of Bromocresol green. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Clarithromycin, Clindamycin, Bromocresol Purple and Bromocresol Green.

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Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ²

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG
2 Department of Medicinal Chemistry, Zagazige University, Zagazig, IN

Abstract

An quantitative spectrophotometric method for determination of cefotaxime Na and cefixime. The developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with DTNB as a chromogenic reagent which could be used for their analysis in pure forms and in pharmaceutical formulations then measuring absorbances at (417nm for cefotaxime and cefixime at 411 nm). The concentration of NaOH, DTNB concentration and hydrolysis time were studied. Calibration curves were linear over ranges of 0.8-4 μg.ml^-1 for Cefotaxime and 0.4- 2 μg.ml^-1 for Cefixime. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Cefotaxime, Cefixime, NaOH, DTNB.

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