A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Raj, Hasumati A.
- First Derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Argatroban in Synthetic Mixture
Authors
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 5, No 1 (2015), Pagination: 18-26Abstract
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using first order derivative zero-crossing method. Edaravone showed zero crossing point at 351.00 nm while Argatroban showed zero crossing point at 280.47 nm. The dA/dλ was measured at 280.47 nm for Edaravone and 351.00nm for Argatroban and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.998) in the range of 10-35μg/ml for Edaravone at 280.47 nm. The linear correlation was obtained (r2>0.999) in the range of 10-35 μg/ml for Argatroban at 351.0 nm. The limit of determination was 1.59μg/ml and 1.87μg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 4.83 μg/ml and 5.68 μg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.Keywords
Edaravone, Argatroban , First Derivative Method, Spectroscopic Method.- Simultaneous Determination of Pravastatin and Valsartan in Synthetic Mixture Using Spectrophotometric Technique (Simultaneous Equation Method)
Authors
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 5, No 1 (2015), Pagination: 27-35Abstract
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using first order derivative zero-crossing method. Edaravone showed zero crossing point at 351.00 nm while Argatroban showed zero crossing point at 280.47 nm. The dA/dλ was measured at 280.47 nm for Edaravone and 351.00nm for Argatroban and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.998) in the range of 10-35μg/ml for Edaravone at 280.47 nm. The linear correlation was obtained (r2>0.999) in the range of 10-35 μg/ml for Argatroban at 351.0 nm. The limit of determination was 1.59μg/ml and 1.87μg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 4.83 μg/ml and 5.68 μg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.- Absorbance Correction Method for Simultaneous Estimation of Edaravone and Argatroban in Synthetic Mixture
Authors
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 5, No 1 (2015), Pagination: 41-48Abstract
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Argatroban in synthetic mixture using Absorbance correction method. At 333 nm (λmax of Argatroban) Edaravone has zero absorbance so Argatroban is directly estimate at 333 nm. At 242 nm (λmax of Edaravone) both drugs have some absorbance so Edaravone is estimate at 242 nm using absorbance correction method.
The method was found to be linear (r2>0.992) in the range of 10-35μg/ml for Edaravone at 242 nm. The linear correlation was obtained (r2>0.998) in the range of 10-35 μg/ml for Argatroban at 333 nm. The limit of determination was 0.060 μg/ml and 0.208μg/ml for Edaravone and Argatroban, respectively. The limit of quantification was 0.184μg/ml and 0.631μg/ml for Edaravone and Argatroban, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99%. The method was successfully applied for simultaneous determination of Edaravone and Argatroban in binary mixture.
Keywords
Edaravone, Argatroban, Absorbance Correction Method, Spectroscopic Method.- Q-Absorbance Ratio Spectrophotometric Method for Simultaneous Determination of Atenolol and Ivabradine HCl in Synthetic Mixture
Authors
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN
2 Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 6, No 1 (2016), Pagination: 27-33Abstract
It describes Simultaneous estimation of simple, accurate, precise, robust and economical Q-Absorbance ratio spectrophotometric method for Atenolol and Ivabradine HCl in synthetic mixture.
Objective: To Delivered information related to Ivabradine HCl and Atenolol combination's analytical method. Both drugs in combination have not any analytical method done by other or nor published anywhere. So we developed and validated it which is novel method for drugs.
Method: Absorbance ratio method for the ratio of absorbance at two selected wavelengths, one which is an isoabsorbtive point and other being the λ-max of one of the two component. Atenolol and Ivabradine HCl show an iso-absorbtive point at 286.40nm in methanol. The second wavelength used is 276nm which is λ-max of Atenolol in methanol. So it is economic in nature. The linearity was obtained in the concentration range of 20-100μg/ml for Atenolol and 2-10μg/ml for Ivabradine HCl. The concentration of the drugs was determined by using ratio of absorbance at iso-absorbtive point and at the λ-max of Atenolol.
Result: This method is linear for both drugs; in range 2-10μg/ml for Ivabradine HCl and 20-100μg/ml for Atenolol found at λmax of Atenolol 276nm (R2=0.9990) and at Iso-absorptive point 286.40nm (R2=0.9998). % recovery for Ivabradine HCl found 100.47% and Atenolol 100.32%. And all validation parameter (Robustness, Ruggedness, Interday, Intraday) show %RSD >2%. And Limit of detection for Ivabradine HCl and Atenolol at λ1(maximum wavelength) and λ2(Iso-absorptive point) was found 0.309 and 0.181 respectively. % Assay for Ivabradine HCl and Atenolol found to be 100.58% and 100.13% respectively.
Conclusion: The method was successfully applied to pharmaceutical synthetic mixture which is considered in approved patent which show no interference. The result of analysis has been validated statistically and by recovery studies. So this method accurate, precise, robust, rugged and economic in nature.
Keywords
Atenolol, Ivabradine HCl, Absorbance Ratio Method, Iso-Absorbtivepoint.- Development and Validation of Stability Indicating High Performance Liquid Chromatographic Method for Olmesartan Medoxomil and Indapamide in Tablet Dosage Form
Authors
1 Department of Quality Assurance, Shree Dhanvantry Pharmacy College, Kim, Surat, Gujarat, IN
2 Dharmaj Degree of Pharmacy, Dharmaj, Anand, Gujarat, IN