Gastroprotective Influence of Vitamin C and its Combination with Rabeprazole in Pylorus Ligation Induced Ulcers
Aim: To assess the influence of Vitamin C (antioxidant) and its combination with rabeprazole in the treatment of peptic ulcer in pylorus ligated rats.
Methodology: The study was divided into 2 dose regimen groups - single and multiple doses. Each regimen group consists of Wistar albino rats of either sex (180-220g) that were divided into 4 groups of 6 animals in each. In single dose regimen group, the animals were fasted for 48 h, after which the drugs and their combination were administered. After 1 h of drug administration the pylorus ligation was performed. However, in multiple dose regimens, the drug and their combination were administered for 07 consecutive days. On 7th day following drug administration the animals were fasted for 48 h and pylorus ligation was performed under ether anesthesia. In both dose regimen groups, the following biochemical parameters were evaluated - volume of gastric juice (ml), pH, free acidity (mEq/L), total acidity (mEq/L) and ulcer index. Histopathology was performed on the stomachs treated with multiple dose regimens.
Results: The interpretation of the results was done statistically using one way ANOVA by Dunnett's test. It was found that the group treated with combination of rabeprazole and vitamin C exhibited significant enhancement of antiulcer activity in both regimen groups on comparison with groups treated with rabeprazole and vitamin C individually.
Conclusion: The combination of vitamin C and rabeprazole was found to be synergistic in nature and more effective in treating peptic ulcer disease upon comparison with standard rabeprazole alone.
- Marshall BJ, Warron JR. Unidentified curved bacilli in stomach of patients with gastritis and peptic ulceration. Lancet. 1984: 1311-15.
- Ronald Fernandez. Ph.D. Dissertation work submitted to the Mangalore University. Gastroprotective and antisecretory effect of selenomethionine in albino rats 2008: 1.
- Dandagi PM, Patil MB, Mastiholimatu VS, Gadad AP, Kulkarni AR. Antiulcer activity of extracts of calotropis gigantean root extracts in pylorus ligated rat model. Int. J Pharmacol. Biol. Sci. 2008; 2(1): 127-32.
- Sharma HL and Sharma KK. Principles of Pharmacology. 1st Edition, Paras publications, 2007: 368.
- Goodman and Gilman. The pharmacological basis of therapeutics. 10th Edition. New York, McGraw Hill: 2001: 1005-6.
- Barar FSK. Essentials of Pharmacotherapeutics. 3rd Edition. 2003: 538-9.
- Langseth L. 1993. Oxidants, antioxidants and disease prevention. International Life Sciences institute, 1-26.
- Dr Dhrubo Jyoti Sen. Herbal Antioxidants: A Great Hope for future. Pharma Times. 2008; 40(12): 22-36.
- Goodman and Gilman. The pharmacological basis of therapeutics. 10th Edition. New York, McGraw Hill: 2001: 1007.
- Shay M, Kemerovo SA, Fels D, Meranze D, Gruenstein H and Siplet: A simple method for uniform production of gastric ulceration in rat. Gastroenterology 5: (1945); 43-61.
- Kulkarni SK. Hand book of experimental pharmacology 3rd edition, Vallabh Prakashan, New Delhi. 2005:148-50.
- Fathihah B, Mahmood AA, Sidik K and Salmah I. The antiulcer and cytoprotective effect of ageratum conyzoides–honey combination in rats, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, JUMMEC 2003-2005; 8: 28-32.
- Deshpande SS, Shah GB, Parmar NS. Antiulcer activity of Tephrosia purpurea in rat. Ind. J of pharmacology. 2003; 35:168-72.
Abstract Views: 107
PDF Views: 2