Asian Journal of Research in Pharmaceutical Sciences

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Free Radical Scavenging and Antioxidant Activity of Punica Granatum Linn


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1 KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, University of Pune, Maharashtra, India
     

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Free radical scavenging activity of Punica granatum Linn. was carried out by DPPH (1, 1-diphenyl-2-picryl hydrazyl) method, Reducing power assay, Scavenging activity against hydrogen peroxide, Total antioxidant activity, Estimation of total flavonoid content in vitro methods. Punica granatum Linn (PG) is rich source of two types of polyphenolic compounds: anthocynins such as delphidin, cyanidin, and pelargonidin which give the fruit and the juice its red colour; and hydrolysable tannins such as punicalin, pedunculagin, gallic and ellagic acid esters of glucose which accounts for 92% of the antioxidant activity of the whole fruit. Oxidative stress (ost) depicts the existence of products called free radicals (molecules possessing an unpaired electron) and reactive oxygen species (ros), which are formed, in normal physiology but become deleterious when not being quenched by a cascade of antioxidants systems (aos). Antioxidant plays an important role in inhibiting and scavenging free radicals thus providing protection to human against infections and degenerative diseases. In the present study Punica granatum Linn found the excellent antioxidant activity as measured by various methods such as DPPH radical scavenging activity, reducing power assay, H2O2 scavenging activity and total antioxidant activity.

Keywords

Antioxidant, DPPH, Flavonoids, Oxidative Stress.
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  • Wiernsperger NF. 2003. Oxidative stress as a therapeutic target in diabetes, revisiting the controversy. Diabetes Metab 29, 579-85.

  • Duke AJ, Ayensu SE. Medicinal plants of China. Algonac, MI, Reference Publications; 1985.

  • Boulos L. Medicinal plants of North Africa. Algonac, MI, Reference Publications; 1983.

  • Ayensu SE. Medicinal plants of West Indies. Algonac, MI, Reference Publications; 1981.

  • Bianchini F, Corbetta F. Health plants of the world. New York, Newsweek; 1979.

  • Fengehun H, Liu X. Chinese patent 1145793A, 1997.

  • Molyneux P. 2004. Use of DPPH to testing antioxidant activity. J. Sci. Technol 26(2), 212-219.

  • Keto K, Terao S, Shimamoto N, Hirata M. 1988. Studies on scavengers of active oxygen species. J. Med. Chem 31, 793-798.

  • Siddhuraju P, Mohan PS, Becker K. 2002. Studies on the antioxidant activit of Indian Laburnum (Cassia fistula L.), A preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem 79, 61–67.

  • Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. 2002. Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NKI. J Agricult Food Chem 50, 2454–2458.

  • Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen 10, 1003–1008.

  • Mistuda H, Yuasumoto K, Iwami K. 1996. Antioxidant action of indole compounds during the autooxidation of linolinic acid. Nihon Eiyo Shokuryo Gakkai-Shi 19, 210-4.

  • Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoids content in Propolis by two complementary colorimetric methods. J Food Drug Anal 10,178-182.

  • Slinkard K, Singleton VL. 1977. Total phenol analyses, Automation and comparison with manual methods. Am J Enol Viticulture 28, 49-55.

  • Polizio AH, Gironacci MM, Tomaro ML, Pena C. 2007. Angiotensin- (1–7) blocks the angiotensin II-stimulated superoxide production. Pharmacol Res 56, 86–90.

  • Cai H, Griendling KK, Harrison DG. 2003. The vascular NAD(P)H oxidases as therapeutic targets in cardiovascular diseases. Trends Pharmacol Sci 24,471–478.

  • McIntyre M, Bohr DF, Dominiczak AF. 1999. Endothelial function in hypertension, the role of superoxide anion. Hypertension 34, 539–545.

  • Kitiyakara C, Wilcox CS. 1998. Antioxidants for hypertension. Curr Opi Nephrol Hypertens 7, 531–538.

  • Duffy SL, Gocke N, Holbrook M, Huang A, Frei B, Keaney JF Jr., et al., 1999. Treatment of hypertension with ascorbic acid. Lancet 354, 2048–2049.

  • Napoli C, Ignarro LJ. 2001. Nitric oxide and atherosclerosis. Nitric Oxide 5, 88-97


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  • Free Radical Scavenging and Antioxidant Activity of Punica Granatum Linn

Abstract Views: 246  |  PDF Views: 2

Authors

Ghanshyam B. Jadhav
KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, University of Pune, Maharashtra, India
Ravindranath B. Saudagar
KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, University of Pune, Maharashtra, India

Abstract


Free radical scavenging activity of Punica granatum Linn. was carried out by DPPH (1, 1-diphenyl-2-picryl hydrazyl) method, Reducing power assay, Scavenging activity against hydrogen peroxide, Total antioxidant activity, Estimation of total flavonoid content in vitro methods. Punica granatum Linn (PG) is rich source of two types of polyphenolic compounds: anthocynins such as delphidin, cyanidin, and pelargonidin which give the fruit and the juice its red colour; and hydrolysable tannins such as punicalin, pedunculagin, gallic and ellagic acid esters of glucose which accounts for 92% of the antioxidant activity of the whole fruit. Oxidative stress (ost) depicts the existence of products called free radicals (molecules possessing an unpaired electron) and reactive oxygen species (ros), which are formed, in normal physiology but become deleterious when not being quenched by a cascade of antioxidants systems (aos). Antioxidant plays an important role in inhibiting and scavenging free radicals thus providing protection to human against infections and degenerative diseases. In the present study Punica granatum Linn found the excellent antioxidant activity as measured by various methods such as DPPH radical scavenging activity, reducing power assay, H2O2 scavenging activity and total antioxidant activity.

Keywords


Antioxidant, DPPH, Flavonoids, Oxidative Stress.

References