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A Kind of Plasma Membrane Intrinsic Protein in Astragalus membranaceus
Astragalus membranaceus is a kind of commonly used herb in traditional Chinese medicine. Plasma membrane intrinsic protein is one of its active ingredients which have important function. In this paper, several characters of Plasma membrane intrinsic protein are studied from the perspective of bioinformatics. First, the composition, the isoelectric point and the hydrophobicity characteristics of the Plasma membrane intrinsic protein are studied by bioinformatics tools. The results show that the molecular weight of plasma membrane intrinsic protein in Astragalus membranaceus is 30244.19, the theoretical isoelectric point is 8.28, the instability index is 30, its structure is stable and there are six transmembrane regions in the Plasma membrane intrinsic protein. Second, there are nine helixes and four strands in the secondary structure of Plasma membrane intrinsic protein. Finally, phosphorylation sites of Plasma membrane intrinsic protein have also been studied via netphos tool, and the result show that it has 23 phosphorylation sites (potential) in Plasma membrane intrinsic protein. This paper summarizes the status and functional usage of Astragalus membranaceus in the development of traditional Chinese medicine.
Plasma Membrane Intrinsic Protein, Astragalus membranaceus, Bioinformatics Analysis.
- Arnaud Besserer, Emeline Burnotte, Gerd Patrick Bienert, et al. Selective Regulation of Maize Plasma Membrane Aquaporin Trafficking and Activity by the SNARE SYP121. Plant Cell. 2012, 24(8): 3463-3481
- Francis J. Alenghat, David E. Golan. Membrane Protein Dynamics and Functional Implications in Mammalian Cells. Curr Top Membr, 2013, 72: 89-120.
- Tanmay Sanjeev Chavan, Serena Muratcioglu, Richard Marszalek, et al. Plasma membrane regulates Ras signaling networks. Cell Logist. 2015, 5(4): e1136374.
- Gene A Morrill, Adele B Kostellow, Richard D Moore, et al. Plasma membrane events associated with the meiotic divisions in the amphibian oocyte: insights into the evolution of insulin transduction systems and cell signaling. BMC Developmental Biology, 2013, 13(1): 3.
- Katja Bernfur, Olaf Larsson, Christer Larsson, et al. Relative Abundance of Integral Plasma Membrane Proteins in Arabidopsis Leaf and Root Tissue Determined by Metabolic Labeling and Mass Spectrometry. PLoS One. 2013, 8(8): e71206.
- Fubito Nakatsu, Jeremy M. Baskin, Jeeyun Chung, et al. PtdIns4P synthesis by PI4KIIIα at the plasma membrane and its impact on plasma membrane identity. J Cell Biol. 2012, 199(6): 1003-1016.
- Enrico Klotzsch, Gerhard J. Schütz. A critical survey of methods to detect plasma membrane rafts. Philos Trans R Soc Lond B Biol Sci. 2013, 368(1611): 20120033.
- Irina D Pogozheva, Henry I Mosberg, Andrei L Lomize. Life at the border: Adaptation of proteins to anisotropic membrane environment. Protein Sci. 2014, 23(9): 1165-1196.
- Markus Babst. Quality control at the plasma membrane: One mechanism does not fit all. J Cell Biol. 2014, 205(1): 11-20.
- Katerina C. Nastou, Georgios N. Tsaousis, Kimon E. Kremizas, et al. The Human Plasma Membrane Peripherome: Visualization and Analysis of Interactions. Biomed Res Int. 2014; 2014: 397145.
- Ning Yang, Tai Wang. Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice. BMC Plant Biol. 2017; 17(1): 2.
- Pablo Mateos-Gil, Sebastian Letschert, Sören Doose, et al. Super-Resolution Imaging of Plasma Membrane Proteins with Click Chemistry. Front Cell Dev Biol. 2016, 4: 98.
- Ren Li, Jinfang Wang, Shuangtao Li, et al. Plasma Membrane Intrinsic Proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 Conferring Enhanced Drought Stress Tolerance in Tomato. Sci Rep. 2016, 6: 31814.
- Yuqi Wang, Ruihong Li, Demou Li, et al. NIP1;2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis. Proc Natl Acad Sci U S A. 2017, 114(19): 5047–5052.
- Stefania Averaimo, Ahlem Assali, Oriol Ros, et al. A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors. Nat Commun. 2016; 7: 12896.
- Robert Berkey, Yi Zhang, Xianfeng Ma, et al. Homologues of the RPW8 Resistance Protein Are Localized to the Extrahaustorial Membrane that Is Likely Synthesized De Novo. Plant Physiol. 2017, 173(1): 600–613.
- Gasteiger E, Hoogland C, Gattiker A, et al. Protein Identification and Analysis Tools on the ExPASy Server, Proteomic Protocols Handbook, 2005, 112: 571-607
- Xie Yong, Hong Xiaokun,Yan Renxiang, et al. Bioinformatics analysis of the recombinant rAgaN3 gene of agarase, Chinese Journal of Bioinformatics, 2017, 15(1):16-26
- Petersen T N, Brunak S, Heijne G, et al. SignalP 4.0: discriminating signal peptides from transmembrane regions. Nature Methods, 2011, 8 (10): 785-786
- David T. Jones, Daniel W. A. Buchan, Domenico Cozzetto, et al. PSICOV: precise structural contact prediction using sparse inverse covariance estimation on large multiple sequence alignments. Bioinformatics, 2012, 28(2):184-90
- Elisabeth Gasteiger, Alexandre Gattiker, Christine Hoogland, et al. ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Research, 2003, 31(13):3784-3788
- Michel Schneider, Michael Tognolli, Amos Bairoch.The Swiss-Prot protein knowledgebase and ExPASy: providing the plant community with high quality proteomic data and tools. Plant Physiology and Biochemistry, 2004, 42(12):1013-1021
- Wafa Tombari, Abdeljelil Ghram. Production of a truncated recombinant HA1 for influenza A H9 subtype screening. Biologicals, 2016, 44(6): 546-555
- Naoya Honda, Takashi Tsukamoto, Yuki Sudo. Comparative evaluation of the stability of seven-transmembrane microbial rhodopsins to various physicochemical stimuli. Chemical Physics Letters, 2017, 682: 6-14.
- Sebastian Stolzenberg, Mayako Michino, Michael V. LeVine, et al. Computational approaches to detect allosteric pathways in transmembrane molecular machines. Biochimica et Biophysica Acta (BBA) - Biomembranes, 2016, 1858(7): 1652-1662.
- Yihua Ma, Congyan Liu, Ding Qu, et al. Antibacterial evaluation of sliver nanoparticles synthesized by polysaccharides from Astragalus membranaceus roots. Biomedicine and Pharmacotherapy, 2017, 89: 351-357
- Huatao Li, Xiaoqiu Zhou, Min Wu, et al. The cytotoxicity and protective effects of Astragalus membranaceus extracts and butylated hydroxyanisole on hydroxyl radical-induced apoptosis in fish erythrocytes. Animal Nutrition, 2016, 2(4): 376-382.
- Rui-Zhan Chen, Li Tan, Chen-Guang Jin, et al. Extraction, isolation, characterization and antioxidant activity of polysaccharides from Astragalus membranaceus. Industrial Crops and Products, 2015, 77: 434-443
- Bin Yang, Bing Xiao, Taoyu Sun. Antitumor and immunomodulatory activity of Astragalus membranaceus polysaccharides in H22 tumor-bearing mice. International Journal of Biological Macromolecules, 2013, 62: 287-290.
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