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Determination of LC50 Values of Extracts of Euphorbia hirta Linn and Euphorbia neriifolia Linn Using Brine Shrimp Lethality Assay


Affiliations
1 Appasaheb Birnale college of Pharmacy, South Shivaji nagar, Sangli 416 416, India
2 Rajarambapu College of Pharmacy, Kasegaon, Sangli, India
     

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Brine shrimp lethality assay is an important tool for preliminary assessment of cytotoxicity of herbal drugs. This method is used for the determination of LC50 values of herbal extracts. In this study, extract of Euphorbia hirta Linn and Euphorbia nerifolia Linn, which are reported in traditional literature for their anti-tumour activity, had been selected to test for brine shrimp lethality activity. This experimentation shows that the LC50 of ethyl acetate and acetone extract of Euphorbia hirta Linn and methanolic extract Euphorbia neriifolia Linn are found to be 71.15, 92.15 and 49.55ug/ml respectively.

Keywords

Brine shrimp lethality assay, Euphorbia hirta Linn, Euphorbia neriifolia Linn.
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  • Determination of LC50 Values of Extracts of Euphorbia hirta Linn and Euphorbia neriifolia Linn Using Brine Shrimp Lethality Assay

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Authors

Sandeep B. Patil
Appasaheb Birnale college of Pharmacy, South Shivaji nagar, Sangli 416 416, India
Chandrakant S. Magdum
Rajarambapu College of Pharmacy, Kasegaon, Sangli, India

Abstract


Brine shrimp lethality assay is an important tool for preliminary assessment of cytotoxicity of herbal drugs. This method is used for the determination of LC50 values of herbal extracts. In this study, extract of Euphorbia hirta Linn and Euphorbia nerifolia Linn, which are reported in traditional literature for their anti-tumour activity, had been selected to test for brine shrimp lethality activity. This experimentation shows that the LC50 of ethyl acetate and acetone extract of Euphorbia hirta Linn and methanolic extract Euphorbia neriifolia Linn are found to be 71.15, 92.15 and 49.55ug/ml respectively.

Keywords


Brine shrimp lethality assay, Euphorbia hirta Linn, Euphorbia neriifolia Linn.