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Spectrophotometric Investigations on Cephalosporines by Ellman’s Reagent in Bulk and Dosage Forms


Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
2 Department of Medicinal Chemistry, Zagazige University, Zagazig, India
     

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An quantitative spectrophotometric method for determination of cefotaxime Na and cefixime. The developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with DTNB as a chromogenic reagent which could be used for their analysis in pure forms and in pharmaceutical formulations then measuring absorbances at (417nm for cefotaxime and cefixime at 411 nm). The concentration of NaOH, DTNB concentration and hydrolysis time were studied. Calibration curves were linear over ranges of 0.8-4 μg.ml-1 for Cefotaxime and 0.4- 2 μg.ml-1 for Cefixime. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Cefotaxime, Cefixime, NaOH, DTNB.
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  • Spectrophotometric Investigations on Cephalosporines by Ellman’s Reagent in Bulk and Dosage Forms

Abstract Views: 46  |  PDF Views: 1

Authors

Sobhy M. El-Adl
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Mohamed El. Hossinny El. Sadek
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Marwa Hamdy Hassan
Department of Medicinal Chemistry, Zagazige University, Zagazig, India

Abstract


An quantitative spectrophotometric method for determination of cefotaxime Na and cefixime. The developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with DTNB as a chromogenic reagent which could be used for their analysis in pure forms and in pharmaceutical formulations then measuring absorbances at (417nm for cefotaxime and cefixime at 411 nm). The concentration of NaOH, DTNB concentration and hydrolysis time were studied. Calibration curves were linear over ranges of 0.8-4 μg.ml-1 for Cefotaxime and 0.4- 2 μg.ml-1 for Cefixime. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords


Cefotaxime, Cefixime, NaOH, DTNB.